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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00309
  • pan locus tag?: SAUPAN001862000
  • symbol: SAOUHSC_00309
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00309
  • symbol: SAOUHSC_00309
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 323458..324483
  • length: 1026
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGAATAATAAAGTATTAGTAACCGGTGGTACAGGGTTTGTTGGCATGCGAATTATTTCA
    CGATTATTAGAACAAGGTTATGACGTACAAACGACGATACGTGATTTAAGTAAAGCTGAT
    AAAGTAATTAAAACAATGCAAGACAATGGCATTTCCACAGAGCGATTAATGTTTGTCGAA
    GCGGATTTATCACAAGATGAACATTGGGATGAAGCAATGAAAGATTGCAAGTATGTCTTG
    AGTGTAGCATCTCCGGTGTTTTTCGGTAAAACAGACGATGCAGAAGTGATGGCGAAGCCT
    GCAATTGAAGGTATACAACGTATTTTAAGAGCTGCAGAACATGCGGGTGTTAAACGTGTG
    GTAATGACTGCAAACTTTGGTGCAGTTGGTTTTAGTAATAAAGATAAAAATTCAATCACA
    AATGAAAGTCATTGGACAAATGAAGATGAACCAGGCTTATCAGTATATGAAAAATCAAAA
    TTGTTAGCTGAAAAGGCAGCGTGGGATTTTGTTGAGAATGAAAATACAACAGTAGAATTT
    GCCACAATCAATCCAGTTGCAATTTTTGGGCCATCATTAGATGCACACGTTTCAGGAAGC
    TTTCATTTATTAGAAAATTTATTGAATGGTTCAATGAAACGTGTACCGCAAATTCCGTTA
    AATGTTGTTGATGTGAGAGACGTAGCTGAACTGCACATTTTGGCAATGACAAATGAACAA
    GCTAATGGCAAGCGATTTATTGCGACGGCTGATGGACAAATTAATTTGTTGGAAATTGCA
    AAATTAATTAAAGAAAAGAGACCTGAAATAGCTCAAAAAGTTTCTACTAAAAAATTACCA
    GACTTTATTTTGAGTCTAGGTGCTAAATTTAATCATCAAGCTAAAGAAGGTAAACTTTTA
    TTAGATATGAACCGCAATGTAAGTAACGAACGTGCAAAAACATTACTTGGTTGGGAACCG
    ATTGCGACACAAGAAGAAGCAATTTTAGCAGCTGTCGATAGTATGGCTAAGTATCATTTA
    ATATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1026

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00309
  • symbol: SAOUHSC_00309
  • description: hypothetical protein
  • length: 341
  • theoretical pI: 5.90297
  • theoretical MW: 37776
  • GRAVY: -0.214956

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    hopanoid-associated sugar epimerase (TIGR03466; HMM-score: 119.8)
    NAD dependent epimerase/dehydratase, LLPSF_EDH_00030 family (TIGR04180; HMM-score: 49.5)
    thioester reductase domain (TIGR01746; HMM-score: 47.6)
    MetabolismEnergy metabolismSugarsUDP-glucose 4-epimerase GalE (TIGR01179; EC 5.1.3.2; HMM-score: 39.3)
    UDP-N-acetylglucosamine 4,6-dehydratase (inverting) (TIGR03589; EC 4.2.1.115; HMM-score: 31.6)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesdTDP-glucose 4,6-dehydratase (TIGR01181; EC 4.2.1.46; HMM-score: 26.9)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesADP-glyceromanno-heptose 6-epimerase (TIGR02197; EC 5.1.3.20; HMM-score: 25)
    acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 24.5)
    Hypothetical proteinsConservedTIGR01777 family protein (TIGR01777; HMM-score: 24.2)
    MetabolismFatty acid and phospholipid metabolismBiosynthesis3-oxoacyl-[acyl-carrier-protein] reductase (TIGR01830; EC 1.1.1.100; HMM-score: 21.5)
    MetabolismEnergy metabolismFermentationacetoin reductases (TIGR02415; EC 1.1.1.-; HMM-score: 21.3)
    UDP-N-acetylglucosamine 4,6-dehydratase/5-epimerase (TIGR04130; EC 4.2.1.-,5.1.3.-; HMM-score: 18.6)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesCDP-glucose 4,6-dehydratase (TIGR02622; EC 4.2.1.45; HMM-score: 18)
    3-hydroxybutyrate dehydrogenase (TIGR01963; HMM-score: 17.9)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesdTDP-4-dehydrorhamnose reductase (TIGR01214; EC 1.1.1.133; HMM-score: 16.9)
    2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (TIGR04316; EC 1.3.1.28; HMM-score: 14.6)
    MetabolismFatty acid and phospholipid metabolismBiosynthesisputative 3-oxoacyl-(acyl-carrier-protein) reductase (TIGR01831; HMM-score: 14.2)
    ergot alkaloid biosynthesis protein, AFUA_2G17970 family (TIGR03649; HMM-score: 13.1)
    2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (TIGR03206; EC 1.1.1.-; HMM-score: 12.9)
    Cellular processesCellular processesToxin production and resistanceputative thiazole-containing bacteriocin maturation protein (TIGR03693; HMM-score: 12.6)
    Unknown functionEnzymes of unknown specificityputative quinone oxidoreductase, YhdH/YhfP family (TIGR02823; HMM-score: 12.3)
  • TheSEED:  
    FIG01108282: hypothetical protein 
  • PFAM:
    NADP_Rossmann (CL0063) 3Beta_HSD; 3-beta hydroxysteroid dehydrogenase/isomerase family (PF01073; HMM-score: 89.7)
    Epimerase; NAD dependent epimerase/dehydratase family (PF01370; HMM-score: 68.8)
    NAD_binding_10; NAD(P)H-binding (PF13460; HMM-score: 60.9)
    GDP_Man_Dehyd; GDP-mannose 4,6 dehydratase (PF16363; HMM-score: 50)
    NmrA; NmrA-like family (PF05368; HMM-score: 45.1)
    NAD_binding_4; Male sterility protein (PF07993; HMM-score: 41.2)
    adh_short; short chain dehydrogenase (PF00106; HMM-score: 27.9)
    RmlD_sub_bind; RmlD substrate binding domain (PF04321; HMM-score: 25.4)
    Polysacc_synt_2; Polysaccharide biosynthesis protein (PF02719; HMM-score: 22.5)
    KR; KR domain (PF08659; HMM-score: 18.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.144
    • Ymax_pos: 11
    • Cmax: 0.11
    • Cmax_pos: 27
    • Smax: 0.273
    • Smax_pos: 21
    • Smean: 0.207
    • D: 0.169
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNNKVLVTGGTGFVGMRIISRLLEQGYDVQTTIRDLSKADKVIKTMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAIEGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNEDEPGLSVYEKSKLLAEKAAWDFVENENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQIPLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVSTKKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAVDSMAKYHLI

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SAOUHSC_02298 (SigB*) [3] [4] other strains
    SigB* (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 4.0 4.1

Relevant publications[edit source | edit]