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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00427
  • pan locus tag?: SAUPAN002179000
  • symbol: SAOUHSC_00427
  • pan gene symbol?: sle1
  • synonym:
  • product: autolysin

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00427
  • symbol: SAOUHSC_00427
  • product: autolysin
  • replicon: chromosome
  • strand: +
  • coordinates: 427609..428613
  • length: 1005
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    GTGCAAAAAAAAGTAATTGCAGCTATTATTGGGACAAGCGCGATTAGCGCTGTTGCGGCA
    ACTCAAGCAAATGCGGCTACAACTCACACAGTAAAACCGGGTGAATCAGTGTGGGCAATT
    TCAAATAAGTATGGGATTTCGATTGCTAAATTAAAGTCATTAAACAATTTAACATCTAAT
    CTAATTTTCCCAAACCAAGTACTAAAAGTATCTGGCTCAAGTAATTCTACGAGTAATAGT
    AGCCGTCCATCAACGAACTCAGGTGGCGGATCATACTACACAGTACAAGCAGGCGACTCA
    TTATCATTAATCGCATCAAAATATGGTACAACTTACCAAAACATTATGCGACTTAATGGT
    TTAAATAATTTCTTTATTTATCCAGGTCAAAAATTAAAAGTATCAGGTACTGCTAGCTCA
    AGTAACGCTGCGAGCAATAGTAGCCGTCCATCAACGAACTCAGGTGGCGGATCATACTAT
    ACAGTACAAGCAGGTGACTCATTGTCATTAATTGCATCAAAATATGGTACAACTTATCAA
    AAAATTATGAGCTTAAATGGCTTAAATAATTTCTTTATATATCCGGGTCAAAAATTGAAA
    GTAACTGGTAATGCATCTACGAACTCAGGATCTGCAACAACGACAAATAGAGGTTACAAT
    ACACCAGTATTCAGTCACCAAAACTTATATACATGGGGTCAATGTACATATCATGTATTT
    AATCGTCGTGCTGAAATTGGTAAAGGTATTAGTACTTATTGGTGGAATGCTAATAACTGG
    GATAACGCAGCGGCAGCAGATGGTTACACTATCGACAATAGACCTACTGTAGGTTCTATC
    GCTCAAACAGATGTAGGTTACTATGGTCATGTTATGTTTGTAGAACGTGTAAATAACGAT
    GGTAGTATTTTAGTTTCAGAAATGAACTATTCAGCTGCACCAGGTATTTTAACTTACAGA
    ACGGTACCAGCTTACCAAGTAAATAATTATAGATATATTCACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1005

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00427
  • symbol: SAOUHSC_00427
  • description: autolysin
  • length: 334
  • theoretical pI: 9.98873
  • theoretical MW: 35835.5
  • GRAVY: -0.326048

Function[edit source | edit]

  • reaction:
    EC 3.5.1.28?  ExPASy
    N-acetylmuramoyl-L-alanine amidaseHydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides
    EC 3.2.1.-?  ExPASy
  • TIGRFAM:
    Cellular processesCellular processesSporulation and germinationspore coat assembly protein SafA (TIGR02899; HMM-score: 73.8)
    Cellular processesCellular processesSporulation and germinationstage VI sporulation protein D (TIGR02907; HMM-score: 35.4)
  • TheSEED:  
    Autolysin precursor 
  • PFAM:
    LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 166.3)
    Peptidase_CA (CL0125) CHAP; CHAP domain (PF05257; HMM-score: 49.4)
    no clan definedSPOR; Sporulation related domain (PF05036; HMM-score: 13.3)
    Attacin_C; Attacin, C-terminal region (PF03769; HMM-score: 11.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cellwall
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 10
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: -0.17
    • Signal Peptide Possibility: 1
    • N-terminally Anchored Score: -2
    • Predicted Cleavage Site: TQANAATT
  • SignalP: Signal peptide length 26 aa
    • Ymax: 0.85
    • Ymax_pos: 26
    • Cmax: 0.844
    • Cmax_pos: 26
    • Smax: 0.953
    • Smax_pos: 19
    • Smean: 0.82
    • D: 0.838
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKYGISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLSLIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNAASNSSRPSTNSGGGSYYTVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNASTNSGSATTTNRGYNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRPTVGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYIH

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Junko Kajimura, Tamaki Fujiwara, Sakuo Yamada, Yoshika Suzawa, Tetsuya Nishida, Yoshihiro Oyamada, Ikue Hayashi, Jun-ichi Yamagishi, Hitoshi Komatsuzawa, Motoyuki Sugai
Identification and molecular characterization of an N-acetylmuramyl-L-alanine amidase Sle1 involved in cell separation of Staphylococcus aureus.
Mol. Microbiol.: 2005, 58(4);1087-101
[PubMed:16262792] [WorldCat.org] [DOI] (P p)
Mark J J B Sibbald, Theresa Winter, Magdalena M van der Kooi-Pol, G Buist, E Tsompanidou, Tjibbe Bosma, Tina Schäfer, Knut Ohlsen, Michael Hecker, Haike Antelmann, Susanne Engelmann, Jan Maarten van Dijl
Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus.
J. Bacteriol.: 2010, 192(14);3788-800
[PubMed:20472795] [WorldCat.org] [DOI] (I p)