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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00474
  • pan locus tag?: SAUPAN002242000
  • symbol: SAOUHSC_00474
  • pan gene symbol?: rplY
  • synonym:
  • product: 50S ribosomal protein L25/general stress protein Ctc

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00474
  • symbol: SAOUHSC_00474
  • product: 50S ribosomal protein L25/general stress protein Ctc
  • replicon: chromosome
  • strand: +
  • coordinates: 472189..472842
  • length: 654
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    ATGGCTTCATTAAAGTCAATCATCCGTCAAGGTAAACAAACACGTTCAGATCTTAAACAA
    TTAAGAAAATCTGGTAAAGTACCAGCAGTAGTATACGGTTACGGTACTAAAAACGTGTCA
    GTTAAAGTTGATGAAGTAGAATTCATCAAAGTTATCCGTGAAGTAGGTCGTAACGGTGTT
    ATCGAATTAGGCGTTGGTTCTAAAACTATCAAAGTTATGGTTGCAGACTACCAATTCGAT
    CCACTTAAAAACCAAATTACTCACATTGACTTCTTAGCAATCAATATGAGTGAAGAACGT
    ACTGTTGAAGTACCAGTTCAATTAGTTGGTGAAGCAGTAGGCGCTAAAGAAGGCGGCGTA
    GTTGAACAACCATTATTCAACTTAGAAGTAACTGCTACTCCAGACAATATTCCAGAAGCA
    ATCGAAGTAGACATTACTGAATTAAACATTAACGACAGCTTAACTGTTGCTGATGTTAAA
    GTAACTGGCGACTTCAAAATCGAAAACGATTCAGCTGAATCAGTAGTAACAGTAGTTGCT
    CCAACTGAAGAACCAACTGAAGAAGAAATCGAAGCTATGGAAGGCGAACAACAAACTGAA
    GAACCAGAAGTTGTTGGCGAAAGCAAAGAAGACGAAGAAAAAACTGAAGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    654

Protein[edit source | edit]

Protein Data Bank: 4WCE
Protein Data Bank: 4WFB
Protein Data Bank: 4WF9
Protein Data Bank: 4WFA

General[edit source | edit]

  • locus tag: SAOUHSC_00474
  • symbol: SAOUHSC_00474
  • description: 50S ribosomal protein L25/general stress protein Ctc
  • length: 217
  • theoretical pI: 4.07608
  • theoretical MW: 23787.5
  • GRAVY: -0.341014

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationribosomal protein bL25, Ctc-form (TIGR00731; HMM-score: 168.4)
  • TheSEED:  
    Protein MetabolismProtein biosynthesisRibosome LSU bacterial LSU ribosomal protein L25p 
  • PFAM:
    no clan definedRibosomal_TL5_C; Ribosomal protein TL5, C-terminal domain (PF14693; HMM-score: 86.3)
    Ribosomal_L25p; Ribosomal L25p family (PF01386; HMM-score: 79.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.146
    • Ymax_pos: 18
    • Cmax: 0.109
    • Cmax_pos: 44
    • Smax: 0.276
    • Smax_pos: 11
    • Smean: 0.197
    • D: 0.166
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVDEVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTVEVPVQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVKVTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]