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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00490
  • pan locus tag?: SAUPAN002257000
  • symbol: SAOUHSC_00490
  • pan gene symbol?: folB
  • synonym:
  • product: dihydroneopterin aldolase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00490
  • symbol: SAOUHSC_00490
  • product: dihydroneopterin aldolase
  • replicon: chromosome
  • strand: +
  • coordinates: 488703..489068
  • length: 366
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    ATGCAAGACACAATCTTTCTTAAAGGTATGCGCTTTTATGGATATCATGGTGCTTTATCA
    GCTGAAAATGAAATAGGGCAAATTTTCAAAGTGGATGTAACTTTGAAAGTAGACTTAGCT
    GAAGCTGGGCGTACTGATAATGTTATTGATACAGTTCATTATGGTGAAGTGTTCGAAGAG
    GTTAAATCAATTATGGAAGGTAAGGCCGTTAATTTACTTGAGCATCTAGCTGAACGTATT
    GCAAATCGTATAAATTCACAATATAATCGTGTAATGGAAACGAAAGTGAGAATCACTAAA
    GAAAACCCACCGATTCCGGGTCATTATGATGGAGTAGGTATCGAAATAGTGAGGGAGAAT
    AAATGA
    60
    120
    180
    240
    300
    360
    366

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00490
  • symbol: SAOUHSC_00490
  • description: dihydroneopterin aldolase
  • length: 121
  • theoretical pI: 5.63716
  • theoretical MW: 13734.5
  • GRAVY: -0.367769

Function[edit source | edit]

  • reaction:
    EC 4.1.2.25?  ExPASy
    Dihydroneopterin aldolase7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersFolic aciddihydroneopterin aldolase (TIGR00525; EC 4.1.2.25; HMM-score: 126.6)
    FolB domain (TIGR00526; HMM-score: 104.8)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsFolate and pterinesFolate Biosynthesis Dihydroneopterin aldolase (EC 4.1.2.25) 
  • PFAM:
    THBO-biosyn (CL0334) FolB; Dihydroneopterin aldolase (PF02152; HMM-score: 111)
    no clan definedCactin_mid; Conserved mid region of cactin (PF10312; HMM-score: 12)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.106
    • Ymax_pos: 21
    • Cmax: 0.156
    • Cmax_pos: 21
    • Smax: 0.115
    • Smax_pos: 40
    • Smean: 0.08
    • D: 0.096
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGRTDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKENPPIPGHYDGVGIEIVRENK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]