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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00493
  • pan locus tag?: SAUPAN002260000
  • symbol: lysS
  • pan gene symbol?: lysS
  • synonym:
  • product: lysyl-tRNA synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00493
  • symbol: lysS
  • product: lysyl-tRNA synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 490080..491567
  • length: 1488
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGTCAGAAGAAATGAATGACCAAATGTTGGTTCGACGTCAAAAATTACAAGAATTATAT
    GATCTTGGTATAGACCCGTTTGGTTCTAAATTTGACCGTTCAGGTTTATCTAGTGATTTG
    AAAGAAGAGTGGGACCAGTATTCTAAAGAAGAATTGGTAGAAAAAGAAGCGGATAGTCAT
    GTCGCTATAGCTGGACGATTAATGACTAAGCGTGGTAAAGGTAAAGCAGGATTTGCACAC
    GTTCAGGACTTAGCTGGACAAATTCAAATTTACGTTCGTAAAGATCAAGTTGGCGATGAC
    GAATTTGATTTATGGAAAAATGCTGATTTAGGCGATATCGTTGGTGTTGAAGGTGTAATG
    TTCAAAACAAATACTGGCGAATTATCGGTTAAAGCGAAGAAATTCACGCTACTAACTAAA
    TCATTGCGACCATTACCGGATAAATTCCACGGTTTACAGGATATTGAACAGAGATATCGT
    CAAAGATATTTAGATTTAATTACGAACGAAGATAGCACTCGTACATTTATTAATCGTAGT
    AAAATCATTCAAGAAATGCGTAATTATTTAAATAATAAAGGTTTCTTGGAAGTAGAAACA
    CCTATGATGCACCAAATTGCTGGTGGAGCAGCTGCTAGACCATTTGTAACACATCATAAT
    GCATTAGATGCAACGTTATACATGCGTATTGCTATTGAGTTGCATTTAAAACGTTTAATT
    GTCGGTGGACTTGAAAAAGTATATGAAATTGGTAGAGTATTCCGTAATGAAGGTGTATCA
    ACTAGACATAACCCTGAATTCACAATGATTGAATTATATGAAGCATATGCAGATTATCAT
    GACATTATGGATTTAACAGAATCTATGGTGAGACATATTGCCAATGAAGTGTTAGGTTCT
    GCAAAAGTACAATACAATGGGGAAACGATTGATTTAGAATCTGCTTGGACTCGTTTGCAT
    ATTGTTGATGCTGTAAAAGAAGCTACTGGTGTAGATTTTTATGAAGTTAAAAGTGATGAA
    GAAGCTAAAGCTTTAGCTAAAGAACATGGTATTGAAATTAAAGATACAATGAAATATGGT
    CATATTTTAAATGAATTCTTTGAGCAAAAAGTTGAAGAAACACTTATTCAGCCAACGTTT
    ATCTATGGTCATCCGACTGAAATTTCACCTTTAGCGAAGAAAAATCCTGAAGATCCTAGA
    TTTACTGATCGTTTCGAATTGTTCATTGTAGGTAGAGAGCATGCAAATGCATTTACTGAA
    TTAAATGATCCTATTGATCAAAAAGGTCGTTTTGAAGCGCAACTTGTTGAAAAAGCGCAA
    GGTAATGATGAAGCGCATGAAATGGATGAAGATTACATTGAAGCGTTAGAATATGGTATG
    CCTCCGACAGGTGGTCTTGGTATCGGTATTGACAGATTGGTTATGTTATTAACTGACTCT
    CCATCAATCAGAGACGTATTATTATTCCCTTATATGAGACAAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1488

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00493
  • symbol: LysS
  • description: lysyl-tRNA synthetase
  • length: 495
  • theoretical pI: 4.89349
  • theoretical MW: 56718.8
  • GRAVY: -0.512929

Function[edit source | edit]

  • reaction:
    EC 6.1.1.6?  ExPASy
    Lysine--tRNA ligaseATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationlysine--tRNA ligase (TIGR00499; EC 6.1.1.6; HMM-score: 735)
    Unknown functionGeneralEF-P lysine aminoacylase GenX (TIGR00462; HMM-score: 246.3)
    Genetic information processingProtein synthesistRNA aminoacylationaspartate--tRNA ligase (TIGR00459; EC 6.1.1.-; HMM-score: 166.9)
    Genetic information processingProtein synthesistRNA aminoacylationaspartate--tRNA(Asn) ligase (TIGR00458; EC 6.1.1.23; HMM-score: 166.6)
    Genetic information processingProtein synthesistRNA aminoacylationasparagine--tRNA ligase (TIGR00457; EC 6.1.1.22; HMM-score: 124)
    Genetic information processingProtein synthesistRNA aminoacylationpyrrolysine--tRNA ligase, C-terminal region (TIGR02367; EC 6.1.1.26; HMM-score: 28.5)
    Genetic information processingProtein synthesistRNA aminoacylationphenylalanine--tRNA ligase, alpha subunit (TIGR00468; EC 6.1.1.20; HMM-score: 22.2)
  • TheSEED:  
    Lysyl-tRNA synthetase (class II) (EC 6.1.1.6) 
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2; tRNA synthetases class II (D, K and N) (PF00152; HMM-score: 283.3)
    OB (CL0021) tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 49.1)
    tRNA_synt_II (CL0040) tRNA-synt_2d; tRNA synthetases class II core domain (F) (PF01409; HMM-score: 24.7)
    NADP_Rossmann (CL0063) Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 10
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.095
    • Ymax_pos: 11
    • Cmax: 0.102
    • Cmax_pos: 45
    • Smax: 0.134
    • Smax_pos: 1
    • Smean: 0.081
    • D: 0.089
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEWDQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEFDLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYRQRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTHHNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAYADYHDIMDLTESMVRHIANEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFYEVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLAKKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDEDYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  5. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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