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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00578
  • pan locus tag?: SAUPAN002372000
  • symbol: SAOUHSC_00578
  • pan gene symbol?: mvaD
  • synonym:
  • product: mevalonate diphosphate decarboxylase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00578
  • symbol: SAOUHSC_00578
  • product: mevalonate diphosphate decarboxylase
  • replicon: chromosome
  • strand: +
  • coordinates: 580410..581393
  • length: 984
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    TTGATTAAAAGTGGCAAAGCACGTGCACATACGAATATTGCACTTATAAAATATTGGGGT
    AAAAAAGATGAAGCACTAATCATTCCAATGAATAATAGCATATCTGTTACATTAGAAAAA
    TTTTACACTGAAACGAAAGTCACTTTTAACGACCAGTTAACACAGGATCAATTTTGGTTG
    AATGGTGAAAAGGTTAGTGGCAAAGAATTAGAGAAAATTTCAAAATATATGGATATTGTC
    AGAAATAGAGCTGGCATCGATTGGTATGCAGAAATTGAAAGCGACAATTTTGTACCAACA
    GCAGCAGGGTTGGCTTCATCGGCAAGCGCATATGCAGCTTTAGCAGCAGCTTGTAATCAA
    GCGCTAGACATGCAGCTGTCAGATAAGGATTTATCGAGATTGGCGCGAATTGGTTCGGGT
    TCTGCGTCGCGTAGTATTTATGGTGGATTTGCAGAATGGGAAAAAGGGTATAGTGATGAG
    ACGTCATATGCCGTTCCACTTGAATCGAATCATTTTGAAGATGACCTTGCCATGATATTT
    GTTGTGATTAATCAACATTCTAAAAAGGTACCTAGTCGATATGGTATGTCATTGACACGA
    AACACATCAAGGTTTTATCAATATTGGTTAGATCATATTGATGAAGATTTAGCTGAAGCA
    AAAGCAGCGATTCAAGACAAAGATTTTAAACGCCTTGGTGAAGTAATTGAAGAAAATGGT
    TTGCGTATGCATGCCACGAATCTAGGATCAACACCGCCGTTCACATATCTTGTGCAAGAA
    AGTTATGATGTCATGGCGCTTGTTCACGAATGCCGAGAAGCGGGGTATCCGTGTTATTTT
    ACAATGGATGCGGGACCTAATGTGAAAATACTTGTAGAAAAGAAAAACAAGCAACAGATT
    ATAGATAAATTATTAACACAGTTTGATAATAACCAAATTATTGATAGTGACATTATTGCC
    ACAGGAATTGAAATAATTGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00578
  • symbol: SAOUHSC_00578
  • description: mevalonate diphosphate decarboxylase
  • length: 327
  • theoretical pI: 4.83909
  • theoretical MW: 36822.3
  • GRAVY: -0.34893

Function[edit source | edit]

  • reaction:
    EC 4.1.1.33?  ExPASy
    Diphosphomevalonate decarboxylaseATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO2
  • TIGRFAM:
    MetabolismCentral intermediary metabolismOtherdiphosphomevalonate decarboxylase (TIGR01240; EC 4.1.1.33; HMM-score: 398.5)
    MetabolismCentral intermediary metabolismOthermevalonate kinase (TIGR00549; EC 2.7.1.36; HMM-score: 45.1)
    MetabolismAmino acid biosynthesisAromatic amino acid familyshikimate kinase (TIGR01920; EC 2.7.1.71; HMM-score: 25.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase (TIGR00154; EC 2.7.1.148; HMM-score: 16.9)
    MetabolismAmino acid biosynthesisAspartate familyhomoserine kinase (TIGR00191; EC 2.7.1.39; HMM-score: 16.2)
    MetabolismEnergy metabolismSugarsgalactokinase (TIGR00131; EC 2.7.1.6; HMM-score: 16)
  • TheSEED:  
    Fatty Acids, Lipids, and IsoprenoidsIsoprenoidsArchaeal lipids Diphosphomevalonate decarboxylase (EC 4.1.1.33) 
    IsoprenoidsIsoprenoid Biosynthesis Diphosphomevalonate decarboxylase (EC 4.1.1.33) 
  • PFAM:
    S5 (CL0329) GHMP_kinases_N; GHMP kinases N terminal domain (PF00288; HMM-score: 31.9)
    no clan definedGHMP_kinases_C; GHMP kinases C terminal (PF08544; HMM-score: 30.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.67
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.137
    • Ymax_pos: 11
    • Cmax: 0.117
    • Cmax_pos: 44
    • Smax: 0.277
    • Smax_pos: 1
    • Smean: 0.166
    • D: 0.149
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTETKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAAGLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDETSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLAEAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGYPCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SAOUHSC_02298 (SigB*) [4] other strains
    SigB* (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 4.0 4.1

Relevant publications[edit source | edit]