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NCBI: 01-MAR-2011 (discontinued in NCBI 03-AUG-2016)

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00814
  • pan locus tag?: SAUPAN002791000
  • symbol: SAOUHSC_00814
  • pan gene symbol?: vwb
  • synonym:
  • product: truncated secreted von Willebrand factor-binding protein (coagulase) VWbp

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00814
  • symbol: SAOUHSC_00814
  • product: truncated secreted von Willebrand factor-binding protein (coagulase) VWbp
  • replicon: chromosome
  • strand: +
  • coordinates: 796163..797515
  • length: 1353
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    TTGGATGATTTAATATGGTCCTTTCCAAACTTAGATAATGAAAGATTTGATAATCCTGAA
    TATAAAGAAGCTATGAAAAAATATCAACAGAGATTTATGGCTGAAGATGAGGCTTTGAAG
    AAATTTTTTAGTGAAGAGAAAAAAATAAAAAATGGAAATACTGATAATTTAGATTATCTA
    GGATTATCTCATGAAAGATATGAAAGTGTATTTAATACTTTGAAAAAACAAAGTGAGGAG
    TTCTTAAAAGAAATTGAAGATATAAAAAAAGATAACCCTGAATTGAAAGACTTTAATGAA
    GAGGAGCAATTAAAGTGCGACTTAGAATTAAACAAATTAGAAAATCAGATATTAATGTTA
    GGTAAAACATTTTATCAAAACTATAGAGATGATGTTGAAAGTTTATATAGTAAGTTAGAT
    TTAATTATGGGATATAAAGATGAAGAAAGAGCAAATAAAAAAGCAGTTAACAAAAGGATG
    TTAGAAAATAAAAAAGAAGACTTAGAAACCATAATTGATGAATTTTTTAGTGATATAGAT
    AAAACAAGACCTAATAATATTCCTGTTTTAGAAGATGAAAAACAAGAAGAGAAAAATCAT
    AAAAATATGGCTCAATTAAAATCTGACACTGAAGCAGCAAAAAGTGATGAATCAAAAAGA
    AGCAAGAGAAGTAAAAGAAGTTTAAATACTCAAAATCACAAACCTGCATCTCAAGAAGTT
    TCTGAACAACAAAAAGCTGAATATGATAAAAGAGCAGAAGAAAGAAAAGCGAGATTTTTG
    GATAATCAAAAAATTAAGAAAACACCTGTAGTGTCATTAGAATATGATTTTGAGCATAAA
    CAACGTATTGACAACGAAAACGACAAGAAACTTGTGGTTTCTGCACCAACAAAGAAACCA
    ACATCACCGACTACATATACTGAAACAACGACACAGGTACCAATGCCTACAGTTGAGCGT
    CAAACTCAGCAACAAATTATTTATAATGCACCAAAACAATTGGCTGGATTAAATGGTGAA
    AGTCATGATTTCACAACAACGCATCAATCACCAACAACTTCAAATCACACGCATAATAAT
    GTTGTTGAATTTGAAGAAACGTCTGCTTTACCTGGTAGAAAATCAGGATCACTGGTTGGT
    ATAAGTCAAATTGATTCTTCTCATCTAACTGAACGTGAGAAGCGTGTAATTAAGCGTGAA
    CACGTTAGAGAAGCTCAAAAGTTAGTTGATAATTATAAAGATACACATAGTTATAAAGAC
    CGAATAAATGCACAACAAAAAGTAAATACTTTAAGTGAAGGTCATCAAAAACGTTTTAAT
    AAACAAATCAATAAAGTATATAATGGCAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1353

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00814
  • symbol: SAOUHSC_00814
  • description: truncated secreted von Willebrand factor-binding protein (coagulase) VWbp
  • length: 450
  • theoretical pI: 6.8153
  • theoretical MW: 52842.4
  • GRAVY: -1.27556

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Virulence, Disease and DefenseAdhesionAdhesins in Staphylococcus Secreted von Willebrand factor-binding protein VWbp 
  • PFAM:
    no clan definedCoagulase; Staphylococcus aureus coagulase (PF08764; HMM-score: 69)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0.1
    • Cytoplasmic Membrane Score: 0.14
    • Cellwall Score: 0.15
    • Extracellular Score: 9.6
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.11
    • Ymax_pos: 25
    • Cmax: 0.129
    • Cmax_pos: 25
    • Smax: 0.123
    • Smax_pos: 41
    • Smean: 0.074
    • D: 0.096
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MDDLIWSFPNLDNERFDNPEYKEAMKKYQQRFMAEDEALKKFFSEEKKIKNGNTDNLDYLGLSHERYESVFNTLKKQSEEFLKEIEDIKKDNPELKDFNEEEQLKCDLELNKLENQILMLGKTFYQNYRDDVESLYSKLDLIMGYKDEERANKKAVNKRMLENKKEDLETIIDEFFSDIDKTRPNNIPVLEDEKQEEKNHKNMAQLKSDTEAAKSDESKRSKRSKRSLNTQNHKPASQEVSEQQKAEYDKRAEERKARFLDNQKIKKTPVVSLEYDFEHKQRIDNENDKKLVVSAPTKKPTSPTTYTETTTQVPMPTVERQTQQQIIYNAPKQLAGLNGESHDFTTTHQSPTTSNHTHNNVVEFEETSALPGRKSGSLVGISQIDSSHLTEREKRVIKREHVREAQKLVDNYKDTHSYKDRINAQQKVNTLSEGHQKRFNKQINKVYNGK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]