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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00849
  • pan locus tag?: SAUPAN002897000
  • symbol: SAOUHSC_00849
  • pan gene symbol?: sufS
  • synonym:
  • product: aminotransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00849
  • symbol: SAOUHSC_00849
  • product: aminotransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 819904..821154
  • length: 1251
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    GTGAATGAAGTGGCCGAACACTCATTTGACGTTAATGAAGTAATCAAGGATTTTCCGATA
    TTAGATCAAAAAGTCAATGGCAAACGTTTAGCATATCTTGATTCAACAGCGACAAGTCAA
    ACGCCTGTGCAAGTGTTAAATGTTTTAGAAGATTACTACAAGCGTTATAATTCAAACGTT
    CATCGTGGTGTTCATACATTAGGATCATTGGCAACTGATGGTTATGAAAATGCCCGTGAA
    ACCGTTCGTCGTTTTATTAATGCGAAGTATTTTGAAGAAATCATTTTTACACGCGGAACA
    ACTGCGTCGATTAACCTTGTAGCACATAGCTATGGTGATGCAAATGTTGAAGAGGGCGAT
    GAAATTGTTGTCACTGAAATGGAACATCATGCCAATATTGTTCCTTGGCAACAGTTAGCA
    AAGCGTAAAAATGCGACATTGAAATTTATACCAATGACAGCTGACGGTGAATTAAACATC
    GAGGATATTAAGCAAACGATTAATGATAAAACAAAGATCGTTGCTATTGCACATATATCT
    AATGTGCTCGGTACAATTAATGATGTTAAAACCATTGCAGAAATAGCTCATCAACATGGT
    GCAATTATCAGTGTTGATGGGGCGCAAGCAGCACCACATATGAAACTTGATATGCAAGAA
    ATGAATGCTGATTTTTATAGTTTTAGTGGTCATAAAATGCTTGGACCAACAGGTATTGGC
    GTATTATTTGGTAAACGTGAGTTACTACAAAAAATGGAACCGATTGAGTTCGGTGGCGAC
    ATGATTGATTTTGTAAGTAAGTATGATGCAACATGGGCTGATTTACCTACTAAATTTGAG
    GCGGGTACTCCATTAATTGCTCAAGCAATTGGGCTTGCAGAAGCTATTCGCTATTTAGAA
    CGCATAGGTTTTGATGCAATTCATAAATATGAACAAGAATTAACGATATATGCTTATGAG
    CAAATGTCTGCAATTGAAGGAATTGAAATTTATGGCCCGCCAAAGGATCGTCGTGCAGGT
    GTAATAACGTTTAATTTACAAGATGTACATCCACACGATGTTGCTACAGCCGTAGATACA
    GAAGGTGTAGCGGTTAGAGCTGGGCATCATTGTGCGCAACCGTTAATGAAATGGTTAAAT
    GTGTCTTCAACAGCTAGAGCGAGTTTTTATATATACAACACGAAAGAAGACGTTGATCAG
    TTAATAAATGCCTTGAAACAAACGAAGGAGTTTTTCTCTTATGAATTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1251

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00849
  • symbol: SAOUHSC_00849
  • description: aminotransferase
  • length: 416
  • theoretical pI: 5.18484
  • theoretical MW: 46618.4
  • GRAVY: -0.257933

Function[edit source | edit]

  • reaction:
    EC 2.8.1.7?  ExPASy
    Cysteine desulfuraseL-cysteine + acceptor = L-alanine + S-sulfanyl-acceptor
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase, SufS family (TIGR01979; HMM-score: 614.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase, catalytic subunit CsdA (TIGR03392; EC 2.8.1.7; HMM-score: 385.1)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01976; HMM-score: 260.8)
    Unknown functionEnzymes of unknown specificitycysteine desulfurase family protein (TIGR01977; HMM-score: 251)
    cysteine desulfurase NifS (TIGR03402; EC 2.8.1.7; HMM-score: 167.4)
    Genetic information processingDNA metabolismRestriction/modificationcysteine desulfurase DndA (TIGR03235; EC 2.8.1.7; HMM-score: 144.7)
    Genetic information processingProtein synthesistRNA and rRNA base modificationcysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 135.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthercysteine desulfurase IscS (TIGR02006; EC 2.8.1.7; HMM-score: 135.9)
    cysteine desulfurase, NifS family (TIGR03403; EC 2.8.1.7; HMM-score: 119.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsergothioneine biosynthesis PLP-dependent enzyme EgtE (TIGR04343; EC 4.4.-.-; HMM-score: 70.6)
    2-aminoethylphosphonate aminotransferase (TIGR03301; EC 2.6.1.-; HMM-score: 42.9)
    O-phospho-L-seryl-tRNA:Cys-tRNA synthase (TIGR02539; EC 2.5.1.73; HMM-score: 41.7)
    MetabolismEnergy metabolismAmino acids and amineskynureninase (TIGR01814; EC 3.7.1.3; HMM-score: 33.3)
    MetabolismAmino acid biosynthesisAspartate familyO-succinylhomoserine sulfhydrylase (TIGR01325; EC 4.2.99.-; HMM-score: 33.1)
    MetabolismEnergy metabolismAmino acids and aminesmethionine gamma-lyase (TIGR01328; EC 4.4.1.11; HMM-score: 33)
    MetabolismCentral intermediary metabolismPhosphorus compounds2-aminoethylphosphonate--pyruvate transaminase (TIGR02326; EC 2.6.1.37; HMM-score: 31.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOthertyrosine decarboxylase MnfA (TIGR03812; EC 4.1.1.25; HMM-score: 30.8)
    MetabolismAmino acid biosynthesisAspartate familyO-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 27.5)
    MetabolismAmino acid biosynthesisSerine familyO-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase (TIGR01326; HMM-score: 27.5)
    MetabolismAmino acid biosynthesisAspartate familyO-succinylhomoserine (thiol)-lyase (TIGR02080; EC 2.5.1.48; HMM-score: 27.2)
    Genetic information processingProtein synthesistRNA aminoacylationL-seryl-tRNA(Sec) selenium transferase (TIGR00474; EC 2.9.1.1; HMM-score: 25.6)
    Cellular processesCellular processesBiosynthesis of natural productscapreomycidine synthase (TIGR03947; HMM-score: 25.2)
    MetabolismAmino acid biosynthesisHistidine familyhistidinol-phosphate transaminase (TIGR01141; EC 2.6.1.9; HMM-score: 23)
    cystathionine beta-lyase (TIGR01329; EC 4.4.1.8; HMM-score: 22.5)
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 22)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesTDP-4-keto-6-deoxy-D-glucose transaminase (TIGR02379; HMM-score: 21)
    MetabolismAmino acid biosynthesisAspartate familycystathionine beta-lyase (TIGR01324; EC 4.4.1.8; HMM-score: 18)
    MetabolismEnergy metabolismAmino acids and aminestyrosine aminotransferase (TIGR01264; EC 2.6.1.5; HMM-score: 16.4)
    UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine transaminase (TIGR03588; EC 2.6.1.92; HMM-score: 16)
    MetabolismEnergy metabolismAmino acids and aminesglycine C-acetyltransferase (TIGR01822; EC 2.3.1.29; HMM-score: 15.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotin8-amino-7-oxononanoate synthase (TIGR00858; EC 2.3.1.47; HMM-score: 15.1)
    Unknown functionEnzymes of unknown specificitypyridoxal phosphate enzyme, MJ0158 family (TIGR03576; HMM-score: 15)
    tyrosine/nicotianamine family aminotransferase (TIGR01265; HMM-score: 14.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalamin5-aminolevulinic acid synthase (TIGR01821; EC 2.3.1.37; HMM-score: 14)
  • TheSEED:  
    Alanine biosynthesis Cysteine desulfurase (EC 2.8.1.7) => SufS 
    CBSS-393130.3.peg.794 Cysteine desulfurase (EC 2.8.1.7) => SufS 
    tRNA modification Archaea Cysteine desulfurase (EC 2.8.1.7) => SufS 
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_5; Aminotransferase class-V (PF00266; HMM-score: 537.3)
    Cys_Met_Meta_PP; Cys/Met metabolism PLP-dependent enzyme (PF01053; HMM-score: 44.4)
    DegT_DnrJ_EryC1; DegT/DnrJ/EryC1/StrS aminotransferase family (PF01041; HMM-score: 37.2)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 36.3)
    Beta_elim_lyase; Beta-eliminating lyase (PF01212; HMM-score: 32.5)
    SelA; L-seryl-tRNA selenium transferase (PF03841; HMM-score: 21.3)
    Pyridoxal_deC; Pyridoxal-dependent decarboxylase conserved domain (PF00282; HMM-score: 16.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.128
    • Ymax_pos: 51
    • Cmax: 0.11
    • Cmax_pos: 49
    • Smax: 0.258
    • Smax_pos: 45
    • Smean: 0.097
    • D: 0.116
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQVLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTASINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNIEDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDMQEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLPTKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPPKDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIYNTKEDVDQLINALKQTKEFFSYEF

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01997 (PerR*)
    PerR* (TF) important in Oxidative stress response

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]