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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00909
  • pan locus tag?: SAUPAN003108000
  • symbol: SAOUHSC_00909
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00909
  • symbol: SAOUHSC_00909
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 878826..879650
  • length: 825
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCAACCACATTTAATATGTCTAGACTTAGACGGAACATTATTAAACGATAACAAAGAA
    ATTTCATCATATACTAAACAAGTATTAAATGAATTACAACAACGTGGACACCAAATTATG
    ATTGCGACTGGCAGACCTTATCGTGCAAGTCAAATGTATTATCATGAATTAAATTTAACG
    ACACCAATTGTTAATTTTAATGGCGCTTACGTACATCACCCTAAAGATAAAAACTTCAAA
    ACTTGCCATGAAATTTTAGATTTAGGCATCGCACAAAACATTATTCAAGGATTACAACAA
    TATCAAGTATCGAATATTATAGCAGAAGTGAAAGATTATGTTTTCATTAACAATCATGAT
    CCAAGATTATTTGAAGGTTTTTCAATGGGTAATCCAAGAATTCAAACTGGTAATTTACTT
    GTCCACTTGAAAGAATCCCCTACCTCAATTTTAATTGAAGCCGAAGAAAGTAAAATACCT
    GAAATCAAAAATATGCTTACTCATTTTTATGCCGATCATATTGAGCATCGACGCTGGGGC
    GCACCATTCCCTGTCATTGAAATTGTAAAACTTGGTATTAATAAAGCAAGAGGCATTGAG
    CAAGTTAGACAATTTTTAAATATTGACCGAAATAATATTATTGCATTCGGTGATGAAGAT
    AATGATATTGAAATGATTGAGTACGCGCGTCACGGTGTTGCTATGGAAAATGGTTTGCAA
    GAACTTAAAGATGTAGCGAACAATATTACATTCAACAATAATGAAGATGGCATTGGTCGA
    TATTTGAATGATTTCTTTAATTTAAATATTAGATATTACTGTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    825

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00909
  • symbol: SAOUHSC_00909
  • description: hypothetical protein
  • length: 274
  • theoretical pI: 5.90828
  • theoretical MW: 31668.7
  • GRAVY: -0.419708

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityCof-like hydrolase (TIGR00099; HMM-score: 205.2)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIB (TIGR01484; HMM-score: 93.3)
    phosphoglycolate phosphatase, TA0175-type (TIGR01487; EC 3.1.3.18; HMM-score: 78)
    sucrose-phosphate phosphatase subfamily (TIGR01482; EC 3.1.3.-; HMM-score: 64.9)
    mannosyl-3-phosphoglycerate phosphatase family (TIGR01486; EC 3.1.3.-; HMM-score: 51)
    mannosyl-3-phosphoglycerate phosphatase (TIGR02461; EC 3.1.3.70; HMM-score: 43.9)
    Unknown functionGeneralmannosyl-3-phosphoglycerate phosphatase homolog (TIGR02463; EC 3.1.3.-; HMM-score: 38)
    sucrose-phosphate synthase, sucrose phosphatase-like domain (TIGR02471; HMM-score: 31.3)
    MetabolismAmino acid biosynthesisSerine familyphosphoserine phosphatase SerB (TIGR00338; EC 3.1.3.3; HMM-score: 29.1)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIIA (TIGR01662; HMM-score: 27.1)
    sucrose phosphatase (TIGR01485; EC 3.1.3.24; HMM-score: 26.8)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharides3-deoxy-D-manno-octulosonate 8-phosphate phosphatase, YrbI family (TIGR01670; EC 3.1.3.45; HMM-score: 24)
    Unknown functionEnzymes of unknown specificityHAD phosphoserine phosphatase-like hydrolase, family IB (TIGR01488; HMM-score: 23.9)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IB (TIGR01490; HMM-score: 19.6)
    HAD phosphatase, family IIIC (TIGR01681; HMM-score: 16.6)
    Unknown functionEnzymes of unknown specificityHAD hydrolase, family IIA (TIGR01460; HMM-score: 16.3)
    phosphoserine phosphatase-like hydrolase, archaeal (TIGR01491; HMM-score: 14.5)
  • TheSEED:  
    FIG002540: Haloacid dehalogenase-like hydrolase 
  • PFAM:
    HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 216.4)
    S6PP; Sucrose-6F-phosphate phosphohydrolase (PF05116; HMM-score: 50.9)
    Hydrolase; haloacid dehalogenase-like hydrolase (PF00702; HMM-score: 27.4)
    HAD_2; haloacid dehalogenase-like hydrolase (PF13419; HMM-score: 25.8)
    HAD; haloacid dehalogenase-like hydrolase (PF12710; HMM-score: 24.2)
    Trehalose_PPase; Trehalose-phosphatase (PF02358; HMM-score: 19.9)
    Acid_PPase; Acid Phosphatase (PF12689; HMM-score: 18.8)
    Hydrolase_6; haloacid dehalogenase-like hydrolase (PF13344; HMM-score: 16.9)
    DUF2608; Protein of unknown function (DUF2608) (PF11019; HMM-score: 13.9)
    GT-B (CL0113) Glyco_trans_4_4; Glycosyl transferase 4-like domain (PF13579; HMM-score: 12.2)
    HAD (CL0137) Acid_phosphat_B; HAD superfamily, subfamily IIIB (Acid phosphatase) (PF03767; HMM-score: 11)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 24
    • Cmax: 0.126
    • Cmax_pos: 24
    • Smax: 0.18
    • Smax_pos: 20
    • Smean: 0.116
    • D: 0.121
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATGRPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQVSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIPEIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGDEDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]