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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00936
  • pan locus tag?: SAUPAN003170000
  • symbol: SAOUHSC_00936
  • pan gene symbol?: trfB
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00936
  • symbol: SAOUHSC_00936
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 908438..909424
  • length: 987
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGTTAGTAGCTTTAAATGAAGAAAAGGAACGCGTATTAGCAACTACTGCATTGAGAAAG
    ACACAATATTTTTGTCCGGTGTGTGGCAAGCAAGTTATTTTAAAGCGTGGGCTCAAAGTA
    ATTAGTCATTTTGCACATAAACATTTAGCGGAACAAAAATGTTTTAATAATGAAACGATT
    AAACATTATAAAAGTAAATTGATTTTAGCACAGATGATACAGCAACAAGGATGTAAAGTA
    GAGATAGAGCCATTTTTAAAAGAAATAAAACAAATTCCGGATATTTTGATTAATAATAAA
    TATGTTATTGAGCTACAGTATTCGCCAATTCCTTATAAACAGATTCTTCAACGAACGGAA
    GGTTTAAAGAAAATGGGATATAAAGTAAGTTGGTTATTAAATGATGTTGATTATTGTCAT
    AATAAAGTGAAGTTCAATCATTTTCAAAGTATGTTTATTAATCCATTCACTCGAAAACTT
    CATACGTTCAATTTAGAGAAAAAACAAATAATGATGTTTCAACAAATACAATATTTAGGC
    GGGCACAAATATGTCGCTGAAAAAAGAAATGCCAAAATTAGTGAGTTGTTTAATGAGGCG
    CCTTGTGATTATCATGCTGTTTATAAATTATCAAAGTTCGCAATTAATCAATATATCAAA
    TATTGTCGCTGGCAAAATTCTGTTTTAGAACCCACTTTAAGTGCAATGTATCAATTACAG
    TTAACTGATCAAGAAGTAGTGCACAATTATGGTTATATTTTTCCAGAGCAAATTTATATT
    AAAAATCATCCTATTGAGTGGCAATTACAAGTTGATTTATGGTTAAAGAATGGAAAAAGC
    AAATTAGTAAATGACAATCTTAATTATTTTAAACTGAAAAAATTTATTGTTGCTCTAGAA
    AGTAAAACAGCAATTATAGAAAAACTTATTAACAATTATTTAAATATTTGTTCAGATAAA
    GGTAATGACGTGCAAATTTTGTTCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    987

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00936
  • symbol: SAOUHSC_00936
  • description: hypothetical protein
  • length: 328
  • theoretical pI: 9.71608
  • theoretical MW: 38840.3
  • GRAVY: -0.335671

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    acetoacetyl-CoA reductase (TIGR01829; EC 1.1.1.36; HMM-score: 14.3)
  • TheSEED:  
    DNA MetabolismDNA uptake, competenceGram Positive Competence Competence protein CoiA 
  • PFAM:
    PDDEXK (CL0236) CoiA; Competence protein CoiA-like family (PF06054; HMM-score: 104.4)
    Zn_Beta_Ribbon (CL0167) NOB1_Zn_bind; Nin one binding (NOB1) Zn-ribbon like (PF08772; HMM-score: 13)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.173
    • Ymax_pos: 51
    • Cmax: 0.121
    • Cmax_pos: 20
    • Smax: 0.411
    • Smax_pos: 42
    • Smean: 0.179
    • D: 0.175
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHFAHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYVIELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSMFINPFTRKLHTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKISELFNEAPCDYHAVYKLSKFAINQYIKYCRWQNSVLEPTLSAMYQLQLTDQEVVHNYGYIFPEQIYIKNHPIEWQLQVDLWLKNGKSKLVNDNLNYFKLKKFIVALESKTAIIEKLINNYLNICSDKGNDVQILF

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]