From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00953
  • pan locus tag?: SAUPAN003195000
  • symbol: SAOUHSC_00953
  • pan gene symbol?: ugtP
  • synonym:
  • product: diacylglycerol glucosyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00953
  • symbol: SAOUHSC_00953
  • product: diacylglycerol glucosyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 926875..928050
  • length: 1176
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGTTACTCAAAATAAAAAGATATTGATTATTACTGGCTCATTCGGTAACGGTCATATG
    CAAGTTACACAGAGTATCGTTAATCAACTTAATGATATGAATCTAGACCATTTAAGCGTC
    ATTGAGCACGATTTATTTATGGAAGCTCATCCAATTTTGACTTCTATTTGTAAAAAATGG
    TATATCAATAGCTTTAAATATTTTAGAAATATGTACAAAGGGTTTTATTACAGCCGCCCA
    GATAAACTAGACAAATGTTTTTACAAATACTATGGACTTAATAAGTTAATTAATTTATTG
    ATAAAAGAAAAGCCAGATTTAATATTATTAACGTTTCCTACACCAGTTATGTCGGTACTA
    ACTGAGCAATTTAACATTAATATTCCAGTTGCTACAGTGATGACAGACTATCGCTTACAT
    AAAAACTGGATTACGCCGTATTCAACAAGATATTATGTGGCAACAAAAGAAACGAAACAA
    GACTTCATAGACGTAGGTATTGATCCTTCAACAGTTAAAGTGACAGGTATTCCTATTGAT
    AACAAATTTGAAACGCCTATTAATCAAAAGCAGTGGTTAATAGACAACAACTTAGATCCA
    GATAAGCAAACTATTTTAATGTCAGCTGGTGCATTTGGTGTATCTAAAGGTTTTGACACG
    ATGATTACTGATATATTAGCGAAAAGTGCAAATGCACAAGTAGTTATGATTTGTGGTAAG
    AGCAAAGAGCTAAAGCGTTCTTTAACAGCTAAGTTTAAATCTAACGAGAATGTATTGATT
    CTAGGTTATACCAAACACATGAATGAATGGATGGCATCAAGTCAACTTATGATTACGAAA
    CCTGGTGGTATCACAATAACTGAAGGTTTCGCCCGTTGTATTCCAATGATTTTCCTAAAT
    CCTGCACCTGGTCAAGAGCTTGAAAATGCCCTTTACTTTGAAGAAAAAGGTTTTGGTAAA
    ATCGCTGATACTCCAGAAGAAGCAATAAAAATTGTAGCAAGTTTAACTAACGGTAATGAG
    CAATTAACTAACATGATTAGTACAATGGAACAAGATAAAATTAAGTATGCTACACAGACA
    ATATGTCGAGACTTACTTGATTTAATCGGTCATTCATCACAACCACAAGAGATTTATGGA
    AAGGTTCCTTTATATGCAAGATTCTTCGTTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1176

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00953
  • symbol: SAOUHSC_00953
  • description: diacylglycerol glucosyltransferase
  • length: 391
  • theoretical pI: 8.60534
  • theoretical MW: 44547.5
  • GRAVY: -0.180563

Function[edit source | edit]

  • reaction:
    EC 2.4.1.315?  ExPASy
    Diglucosyl diacylglycerol synthase (1,6-linking)UDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDPUDP-alpha-D-glucose + 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol = 1,2-diacyl-3-O-(beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyl-(1->6)-O-beta-D-glucopyranosyl)-sn-glycerol + UDP
    UDP-glucose + 1,2-diacyl-sn-glycerol = UDP + 1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol?
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanundecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase (TIGR01133; EC 2.4.1.227; HMM-score: 41.9)
    UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose hydrolase (TIGR03590; EC 3.6.1.57; HMM-score: 23.5)
    Hypothetical proteinsConservedconserved hypothetical protein (TIGR00661; HMM-score: 20.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogsN-acetyl-alpha-D-glucosaminyl L-malate synthase BshA (TIGR03999; EC 2.4.1.-; HMM-score: 11.5)
  • TheSEED:  
    Cell Wall and CapsuleGram-Positive cell wall componentsTeichoic and lipoteichoic acids biosynthesis diglucosyldiacylglycerol synthase (LTA membrane anchor synthesis) 
  • PFAM:
    GT-B (CL0113) MGDG_synth; Monogalactosyldiacylglycerol (MGDG) synthase (PF06925; HMM-score: 162.4)
    Glyco_tran_28_C; Glycosyltransferase family 28 C-terminal domain (PF04101; HMM-score: 40.6)
    Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 33)
    Glyco_trans_1_4; Glycosyl transferases group 1 (PF13692; HMM-score: 22.7)
    Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 19.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.186
    • Ymax_pos: 25
    • Cmax: 0.123
    • Cmax_pos: 28
    • Smax: 0.365
    • Smax_pos: 20
    • Smean: 0.266
    • D: 0.218
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLSVIEHDLFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIKEKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQDFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGFDTMITDILAKSANAQVVMICGKSKELKRSLTAKFKSNENVLILGYTKHMNEWMASSQLMITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVASLTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

M Y Kiriukhin, D V Debabov, D L Shinabarger, F C Neuhaus
Biosynthesis of the glycolipid anchor in lipoteichoic acid of Staphylococcus aureus RN4220: role of YpfP, the diglucosyldiacylglycerol synthase.
J. Bacteriol.: 2001, 183(11);3506-14
[PubMed:11344159] [WorldCat.org] [DOI] (P p)
Angelika Gründling, Olaf Schneewind
Genes required for glycolipid synthesis and lipoteichoic acid anchoring in Staphylococcus aureus.
J. Bacteriol.: 2007, 189(6);2521-30
[PubMed:17209021] [WorldCat.org] [DOI] (P p)