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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00958
  • pan locus tag?: SAUPAN003203000
  • symbol: SAOUHSC_00958
  • pan gene symbol?: htrA2
  • synonym:
  • product: serine protease HtrA

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_00958
  • symbol: SAOUHSC_00958
  • product: serine protease HtrA
  • replicon: chromosome
  • strand: +
  • coordinates: 933094..935418
  • length: 2325
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGAGTGGACATTAGTGGATATTGGTAAAAAACATGTAATTCCTAAAAGTCAGTACCGA
    CGTAAGCGTCGTGAATTCTTCCACAACGAAGACAGAGAAGAAAATTTAAATCAACATCAA
    GATAAACAAAATATAGATAATACAACATCAAAAAAAGCAGATAAGCAAATACATAAAGAT
    TCAATTGATAAGCACGAACGTTTTAAAAATAGTTTATCATCGCATTTAGAACAGAGAAAC
    CGTGATGTTAATGAGAATAAAGCTGAAGAAAGTAAAAGTAATCAGGATAGTAAGTCAGCA
    TATAACAGAGATCATTATTTAACAGACGATGTATCTAAAAAACAAAATTCATTAGATTCA
    GTGGACCAAGATACAGAGAAATCAAAATATTATGAGCAAAATTCTGAAGCGACTTTATCA
    ACTAAATCAACCGATAAAGTAGAATCAACTGAAATGAGAAAGCTAAGTTCAGATAAAAAC
    AAAGTTGGTCATGAAGAGCAACATGTACTTTCTAAACCTTCAGAACATGATAAAGAGACT
    AGAATTGATTCTGAGTCTTCAAGAACTGATTCAGACAGCTCGATGCAGACAGAGAAAATA
    AAAAAAGACAGTTCAGATGGAAATAAAAGTAGTAATCTGAAATCTGAAGTAATATCAGAC
    AAATCAAATACAGTACCAAAATTGTCGGAATCTGATGATGAAGTAAATAATCAGAAGCCA
    TTAACTTTACCGGAAGAACAGAAATTGAAAAGACAGCAAAGTCAAAATGAGCAAACAAAA
    ACCTATACATATGGTGATAGCGAACAAAATGACAAGTCTAATCATGAAAATGATTTAAGT
    CATCATATACCATCGATAAGTGATGATAAAGATAACGTCATGAGAGAAAATCATATTGTT
    GACGATAATCCTGATAATGATATCAATACACCATCATTATCAAAAACAGATGACGATCGA
    AAACTTGATGAAAAAATTCATGTTGAAGATAAACATAAACAAAATGCAGACTCGTCTGAA
    ACGGTGGGATATCAAAGTCAGTCAACTGCATCTCATCGTAGCACTGAAAAAAGAAATATT
    TCTATTAATGACCATGATAAATTAAACGGTCAAAAAACAAATACAAAGACATCGGCAAAT
    AATAATCAAAAAAAGGCTACATCAAAATTGAACAAAGGGCGCGCTACGAATAATAATTAT
    AGTGACATTTTGAAAAAGTTTTGGATGATGTATTGGCCTAAATTAGTTATTCTAATGGGT
    ATTATTATTCTAATTGTTATTTTGAATGCCATTTTTAATAATGTGAACAAAAATGATCGC
    ATGAATGATAATAATGATGCAGATGCTCAAAAATATACGACAACGATGAAAAATGCCAAT
    AACACAGTTAAATCGGTCGTTACAGTTGAAAATGAAACATCAAAAGATTCATCATTACCT
    AAAGATAAAGCATCTCAAGACGAAGTGGGATCAGGTGTTGTATATAAAAAATCTGGAGAT
    ACGTTATATATTGTTACGAATGCACACGTTGTCGGTGATAAAGAAAATCAAAAAATAACT
    TTCTCGAATAATAAAAGTGTTGTTGGGAAAGTGCTTGGTAAAGATAAATGGTCAGATTTA
    GCTGTTGTTAAAGCAACTTCTTCAGACAGTTCAGTGAAAGAGATAGCTATTGGAGATTCA
    AATAATTTAGTGTTAGGAGAGCCAATATTAGTCGTAGGTAATCCACTTGGTGTAGACTTT
    AAAGGCACTGTGACAGAAGGTATTATTTCAGGTCTGAACAGAAATGTTCCTATTGATTTC
    GATAAAGATAATAAATATGATATGTTGATGAAAGCTTTCCAAATTGATGCATCAGTAAAT
    CCAGGTAACTCGGGTGGTGCTGTCGTCAATAGAGAAGGAAAATTAATAGGTGTAGTTGCA
    GCTAAAATTAGTATGCCAAACGTTGAAAATATGTCATTTGCAATACCTGTTAATGAAGTA
    CAAAAGATTGTAAAAGATTTAGAAACAAAAGGTAAAATTGACTATCCCGATGTAGGTGTT
    AAAATGAAGAATATTGTCAGTCTAAATAGTTTTGAAAGACAAGCAGTTAAATTGCCAGGA
    AAAGTTAAGAACGGTGTTGTTGTAGATCAAGTTGACAACAATGGTTTAGCAGATCAATCT
    GGTCTGAAAAAAGGTGATGTAATTACTGAATTAGATGGCAAACTTTTAGAAGATGATTTA
    CGCTTTAGGCAGATTATATTTAGTCATAAAGATGACTTGAAATCAATTACAGCGAAGATT
    TATAGAGATGGTAAAGAGAAAGAAATTAATATTAAACTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2325

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_00958
  • symbol: SAOUHSC_00958
  • description: serine protease HtrA
  • length: 774
  • theoretical pI: 6.88081
  • theoretical MW: 87087.9
  • GRAVY: -1.02558

Function[edit source | edit]

  • reaction:
    EC 3.4.21.-?  ExPASy
  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 218.8)
    Genetic information processingProtein fateProtein folding and stabilizationpeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 218.8)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 137.9)
    Signal transductionRegulatory functionsProtein interactionsperiplasmic serine peptidase DegS (TIGR02038; EC 3.4.21.-; HMM-score: 137.9)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesRIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 18)
  • TheSEED:  
    Serine protease, DegP/HtrA, do-like (EC 3.4.21.-) 
  • PFAM:
    Peptidase_PA (CL0124) Trypsin_2; Trypsin-like peptidase domain (PF13365; HMM-score: 106.9)
    PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 42.8)
    Peptidase_PA (CL0124) Trypsin; Trypsin (PF00089; HMM-score: 42.8)
    PDZ-like (CL0466) PDZ; PDZ domain (Also known as DHR or GLGF) (PF00595; HMM-score: 22.4)
    no clan definedDUF4834; Domain of unknown function (DUF4834) (PF16118; HMM-score: 15.2)
    Peptidase_PA (CL0124) Peptidase_S32; Equine arteritis virus serine endopeptidase S32 (PF05579; HMM-score: 13.9)
    no clan definedDUF3671; Protein of unknown function (PF12420; HMM-score: 6.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: Intracellular /TMH start AFTER 60
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: Possibly Sec-
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.122
    • Ymax_pos: 24
    • Cmax: 0.106
    • Cmax_pos: 24
    • Smax: 0.214
    • Smax_pos: 21
    • Smean: 0.121
    • D: 0.122
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLNQHQDKQNIDNTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKAEESKSNQDSKSAYNRDHYLTDDVSKKQNSLDSVDQDTEKSKYYEQNSEATLSTKSTDKVESTEMRKLSSDKNKVGHEEQHVLSKPSEHDKETRIDSESSRTDSDSSMQTEKIKKDSSDGNKSSNLKSEVISDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHENDLSHHIPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQNADSSETVGYQSQSTASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSKLNKGRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDADAQKYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNAHVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLGEPILVVGNPLGVDFKGTVTEGIISGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNSGGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKDLETKGKIDYPDVGVKMKNIVSLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDLRFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]