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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01168
  • pan locus tag?: SAUPAN003481000
  • symbol: pyrC
  • pan gene symbol?: pyrC
  • synonym:
  • product: dihydroorotase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01168
  • symbol: pyrC
  • product: dihydroorotase
  • replicon: chromosome
  • strand: +
  • coordinates: 1116581..1117855
  • length: 1275
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAAATTAATTAAAAACGGTAAAGTATTACAAAATGGCGAATTACAACAAGCAGATATT
    TTAATTGATGGTAAGGTAATTAAACAAATTGCACCTGCAATTGAACCAAGCAATGGTGTT
    GACATCATAGATGCGAAAGGTCACTTTGTGTCACCTGGATTTGTCGATGTTCATGTTCAT
    TTACGTGAACCTGGTGGTGAATATAAAGAGACAATTGAAACTGGTACTAAAGCTGCTGCT
    AGAGGCGGATTTACAACTGTATGTCCAATGCCTAACACAAGACCGGTACCAGATTCTGTA
    GAACATTTTGAAGCTTTACAAAAATTAATCGATGACAATGCTCAAGTACGTGTATTACCT
    TATGCTTCAATTACAACACGTCAATTAGGTAAAGAATTGGTTGATTTCCCAGCACTAGTA
    AAAGAAGGTGCCTTTGCGTTTACAGATGACGGTGTAGGAGTACAAACTGCAAGCATGATG
    TATGAAGGCATGATTGAAGCTGCAAAAGTAAACAAAGCCATCGTAGCACACTGTGAAGAT
    AATTCATTAATCTATGGTGGTGCAATGCATGAAGGGAAACGCAGTAAAGAGTTAGGTATA
    CCAGGTATTCCAAACATTTGTGAATCTGTTCAAATCGCAAGAGATGTACTATTAGCTGAA
    GCAGCAGGTTGTCATTATCATGTATGTCATGTTTCTACTAAAGAAAGTGTTAGAGTCATT
    CGTGACGCTAAACGCGCAGGCATTCATGTTACAGCTGAAGTTACACCACACCATTTATTG
    TTAACAGAAGATGATATTCCTGGTAATAATGCCATTTATAAAATGAATCCACCATTGAGA
    AGTACTGAAGATAGAGAGGCTTTGTTAGAAGGGTTACTAGACGGTACAATTGACTGTATC
    GCAACAGACCATGCACCACATGCACGTGATGAAAAAGCACAACCAATGGAAAAAGCACCA
    TTCGGAATTGTTGGTAGTGAAACAGCATTCCCATTATTATATACGCATTTTGTAAAAAAT
    GGTGATTGGACATTACAACAATTAGTAGATTACTTAACAATTAAACCATGTGAGACATTT
    AATTTAGAATACGGCACATTAAAAGAAAATGGTTATGCAGATTTAACAATCATTGATTTA
    GATAGTGAACAAGAAATTAAAGGAGAAGATTTCTTATCAAAAGCAGATAATACACCATTT
    ATCGGCTATAAAGTTTATGGAAATCCGATCTTAACAATGGTTGAAGGCGAAGTTAAATTT
    GAGGGGGATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1275

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01168
  • symbol: PyrC
  • description: dihydroorotase
  • length: 424
  • theoretical pI: 4.84608
  • theoretical MW: 46371.4
  • GRAVY: -0.22783

Function[edit source | edit]

  • reaction:
    EC 3.5.2.3?  ExPASy
    Dihydroorotase(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate
  • TIGRFAM:
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisdihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 471.8)
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 245.6)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 201.9)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPyrimidine ribonucleotide biosynthesisdihydroorotase, homodimeric type (TIGR00856; EC 3.5.2.3; HMM-score: 47)
    Hypothetical proteinsConservedputative amidohydrolase, EF_0837/AHA_3915 family (TIGR03583; HMM-score: 43.1)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesbeta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 33.8)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesSalvage of nucleosides and nucleotidesadenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 28.1)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 27)
    MetabolismEnergy metabolismOtherphosphonate metabolism protein PhnM (TIGR02318; HMM-score: 26.7)
    MetabolismEnergy metabolismAmino acids and aminesimidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 25.9)
    MetabolismCentral intermediary metabolismAmino sugarsN-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 24.7)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 18.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesOtherguanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 18.2)
  • TheSEED:  
    Nucleosides and NucleotidesPyrimidinesDe Novo Pyrimidine Synthesis Dihydroorotase (EC 3.5.2.3) 
  • PFAM:
    Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 161.9)
    DHOase; Dihydro-orotase-like (PF12890; HMM-score: 120)
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 39.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.123
    • Ymax_pos: 34
    • Cmax: 0.14
    • Cmax_pos: 34
    • Smax: 0.124
    • Smax_pos: 18
    • Smean: 0.1
    • D: 0.114
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIIDAKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEHFEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMMYEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLLAEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMNPPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLYTHFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFLSKADNTPFIGYKVYGNPILTMVEGEVKFEGDK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]