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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01184
  • pan locus tag?: SAUPAN003500000
  • symbol: SAOUHSC_01184
  • pan gene symbol?: sun
  • synonym:
  • product: 16S rRNA (cytosine(967)-C(5))-methyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01184
  • symbol: SAOUHSC_01184
  • product: 16S rRNA (cytosine(967)-C(5))-methyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1135052..1136359
  • length: 1308
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGATAGAAAACGTGAGAAGTCTTGCTTTTGACACGATTCAAGATATATTAAATGAAGGT
    GCGTATAGTAACTTGCGTATCAATGAAGTGTTGTCAGAAAATGAATTAAATGCAATGGAT
    AAGGCTTTATTTACAGAAATTGTCTACGGAACCGTTAAAAGAAAATATACGTTAGATTTT
    TATTTAAAGCCTTTTGTGAAAACAAAGATTAAGGCATGGGTTAGGCAATTATTATGGATG
    AGTATTTATCAATATGTTTATTTAGATAAAGTTCCAAATCATGCCATTATTAATGAAGCA
    GTTGAAATAGCAAAAGAACGCGGTGGCTATCATAATGGTAATGTCGTAAATGGTATTTTA
    CGTACAATGATGCGTAGTGACTTACCTGATTTTAATGAAATTGCAGATCCTAAAAAAAGA
    ATGGCAATCGAGTATAGTATGCCGAAGTGGATTATAGATCATTGGGCAACACATTATGGT
    CTCGAAGAAACTGAAAAAATTTTACAGTCATTTTTAGAAACGACATCAACAACTGTGCGT
    GCCAACCTGACGCGAGCATCATTAGATGATATTATTGAAAAGTTGCAAGACGAAGGTTAT
    GACGTTGAAAAAGATCATGACTTACCTTATTGTCTCCATATAGGAGGACAACCAATTATT
    CATTCTCGTTCATTTAAAGATGGATTCGTTTCAATTCAAGATAAAAGCTCAATGTTTGTT
    GCACACATTATGAATGTAGACCGACATGATCACGTATTAGATGCATGTAGTGCACCTGGC
    GGTAAAGCTTGTCACATTGCTGAAGTTTTAATGCCAGAAGGGCAAGTTGACGCTTCAGAT
    ATACATGATCACAAAATAGACTTAATTAATTTTAATATAAAAAAATTACGATTAACAAAT
    ATTAAAGCTTTTCAACATGATGCGACAAAACCTTATGATAAAACATACGATAAGATACTT
    GTTGATGCACCATGTAGCGGATTAGGTGTAATGAGACATAAGCCGGAGATTAAGTATACT
    CAAAGCAAACAACATATTGAGTCACTAGTTGAATTACAGCTTGAAATATTGGAAAATGTA
    AAAAACAATGTAAAAATAGGTGGAGAAATCATCTATTCAACATGTACAATTGAGCAACTA
    GAAAATGAAAACGTGATTTATACGTTTTTGAAAAATAATAAAAACTTCGAATTTGAACCG
    TTTCAACATCCGATAACTGGAGAGTTGGTCAAAACGTTACAAATCATGCCGCAAGACTTT
    AATTCAGATGGATTCTTTATCACTAAGATAAAAAGAAAGGACAATTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1308

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01184
  • symbol: SAOUHSC_01184
  • description: 16S rRNA (cytosine(967)-C(5))-methyltransferase
  • length: 435
  • theoretical pI: 6.16903
  • theoretical MW: 50114
  • GRAVY: -0.376092

Function[edit source | edit]

  • reaction:
    EC 2.1.1.-?  ExPASy
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modification16S rRNA (cytosine(967)-C(5))-methyltransferase (TIGR00563; EC 2.1.1.176; HMM-score: 395.8)
    Genetic information processingProtein synthesistRNA and rRNA base modificationNOL1/NOP2/sun family putative RNA methylase (TIGR00446; EC 2.1.1.-; HMM-score: 151.1)
    Genetic information processingTranscriptionTranscription factorstranscription antitermination factor NusB (TIGR01951; HMM-score: 57.5)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationprotein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 26)
    Genetic information processingProtein fateProtein modification and repairmethyltransferase, HemK family (TIGR00536; HMM-score: 19.4)
    Genetic information processingProtein fateProtein modification and repairprotein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 19)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 18.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinonedemethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 17.9)
    Genetic information processingProtein synthesistRNA and rRNA base modificationN2,N2-dimethylguanosine tRNA methyltransferase (TIGR00308; EC 2.1.1.-; HMM-score: 17.6)
    Genetic information processingProtein fateProtein modification and repairprotein-L-isoaspartate O-methyltransferase (TIGR00080; EC 2.1.1.77; HMM-score: 14.1)
    Signal transductionRegulatory functionsDNA interactionsbiotin operon repressor (TIGR00122; HMM-score: 13.4)
    Genetic information processingProtein synthesistRNA and rRNA base modificationribosomal RNA large subunit methyltransferase J (TIGR00438; EC 2.1.1.166; HMM-score: 13.2)
    methyltransferase, FxLD system (TIGR04364; HMM-score: 11.2)
  • TheSEED:  
    Conserved gene cluster associated with Met-tRNA formyltransferase Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-) 
  • PFAM:
    NADP_Rossmann (CL0063) Methyltr_RsmB-F; 16S rRNA methyltransferase RsmB/F (PF01189; HMM-score: 174.4)
    NusB (CL0633) NusB; NusB family (PF01029; HMM-score: 108.2)
    NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 34.5)
    FtsJ; FtsJ-like methyltransferase (PF01728; HMM-score: 18.1)
    Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 15.5)
    DUF2431; Domain of unknown function (DUF2431) (PF10354; HMM-score: 14.8)
    Ubiquitin (CL0072) NLE; NLE (NUC135) domain (PF08154; HMM-score: 14.1)
    NADP_Rossmann (CL0063) DUF938; Protein of unknown function (DUF938) (PF06080; HMM-score: 13.9)
    Ubie_methyltran; ubiE/COQ5 methyltransferase family (PF01209; HMM-score: 13.1)
    P-loop_NTPase (CL0023) AAA_31; AAA domain (PF13614; HMM-score: 13)
    NADP_Rossmann (CL0063) TRM; N2,N2-dimethylguanosine tRNA methyltransferase (PF02005; HMM-score: 12.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAOUHSC_00943(ppnK)inorganic polyphosphate/ATP-NAD kinase  [1] (data from MRSA252)
    SAOUHSC_02506(rpsC)30S ribosomal protein S3  [1] (data from MRSA252)
    SAOUHSC_02487(rpsM)30S ribosomal protein S13  [1] (data from MRSA252)
    SAOUHSC_00679hypothetical protein  [1] (data from MRSA252)
    SAOUHSC_01040pyruvate dehydrogenase complex, E1 component subunit alpha  [1] (data from MRSA252)
    SAOUHSC_01041pyruvate dehydrogenase complex, E1 component subunit beta  [1] (data from MRSA252)
    SAOUHSC_01042branched-chain alpha-keto acid dehydrogenase subunit E2  [1] (data from MRSA252)
    SAOUHSC_01043dihydrolipoamide dehydrogenase  [1] (data from MRSA252)
    SAOUHSC_01416dihydrolipoamide succinyltransferase  [1] (data from MRSA252)
    SAOUHSC_01794glyceraldehyde 3-phosphate dehydrogenase 2  [1] (data from MRSA252)
    SAOUHSC_01801isocitrate dehydrogenase  [1] (data from MRSA252)
    SAOUHSC_01806pyruvate kinase  [1] (data from MRSA252)
    SAOUHSC_02849pyruvate oxidase  [1] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.109
    • Ymax_pos: 22
    • Cmax: 0.126
    • Cmax_pos: 22
    • Smax: 0.143
    • Smax_pos: 18
    • Smean: 0.083
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALFTEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVEIAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYGLEETEKILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQPIIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQVDASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHKPEIKYTQSKQHIESLVELQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKNNKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]