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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01192
  • pan locus tag?: SAUPAN003508000
  • symbol: SAOUHSC_01192
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01192
  • symbol: SAOUHSC_01192
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1143605..1143979
  • length: 375
  • essential: no DEG other strains

gene symbol: vfrA [1]

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    ATGACATTAGAGATTTCAAATGATTACGGTAAAATTGATATTTCAAACGAAGTGATTGCT
    TCGGTTGTAGGTGGAAAGGCCGTTGAATGTTATGGTATTGTAGGTATGGCATCTAGACAA
    CAAGTTAGAGATGGTATTGCGGAAATACTAGGACATGAAAATTATGCTAAAGGCATCAAA
    GTAACTGAAAATAATGGCGTAGTGGATATAGATATGTACATTATTGTAAGTTACGGTGTG
    AAAATATCTGAAGTTGCCAATAATGTACAATCAACAGTGAAATATACTTTGGAAAAATCA
    CTTAATGTATCAGTAAATTCAATCAATATATATGTACAAGGTGTACGTGTGAATAATACA
    GGCAAGAAAGCTTAG
    60
    120
    180
    240
    300
    360
    375

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01192
  • symbol: SAOUHSC_01192
  • description: hypothetical protein
  • length: 124
  • theoretical pI: 5.86662
  • theoretical MW: 13385.2
  • GRAVY: 0.000806451

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    A Gram-positive cluster that relates ribosomal protein L28P to a set of uncharacterized proteins FIG001802: Putative alkaline-shock protein 
  • PFAM:
    no clan definedAsp23; Asp23 family, cell envelope-related function (PF03780; HMM-score: 112.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.122
    • Ymax_pos: 47
    • Cmax: 0.129
    • Cmax_pos: 37
    • Smax: 0.19
    • Smax_pos: 35
    • Smean: 0.096
    • D: 0.112
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRDGIAEILGHENYAKGIKVTENNGVVDIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLNVSVNSINIYVQGVRVNNTGKKA

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Jeffrey L Bose, Seth M Daly, Pamela R Hall, Kenneth W Bayles
    Identification of the Staphylococcus aureus vfrAB operon, a novel virulence factor regulatory locus.
    Infect. Immun.: 2014, 82(5);1813-22
    [PubMed:24549328] [WorldCat.org] [DOI] (I p)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]