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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01239
  • pan locus tag?: SAUPAN003563000
  • symbol: SAOUHSC_01239
  • pan gene symbol?: rasP
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01239
  • symbol: SAOUHSC_01239
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1186354..1187538
  • length: 1185
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTGTCCAGAATTTGCGATCGGTATGGGGCCAAAAATTTTTAGTTTTAGAAAAAATGAA
    ACACTTTACACTATTAGGTTATTGCCTGTTGGTGGATATGTTCGTATGGCAGGAGATGGC
    TTAGAAGAGCCACCAGTCGAGCCCGGTATGAACGTTAAAATTAAACTTAATGAAGAAAAT
    GAAATAACACATATCATATTAGATGATCATCATAAGTTTCAACAAATTGAAGCGATCGAA
    GTTAAAAAATGTGATTTTAAGGATGACTTATTCATAGAAGGTATCACTGCTTATGATAAT
    GAAAGACATCATTTTAAAATTGCTAGAAAGTCTTTCTTTGTTGAAAATGGTAGCTTAGTT
    CAAATTGCTCCGAGAGACAGACAATTTGCACATAAAAAGCCATGGCCGAAATTTTTAACA
    TTATTTGCGGGACCGTTATTTAACTTTATATTAGCTTTAGTCCTATTTATTGGTCTTGCA
    TATTATCAAGGCACGCCTACGTCTACTGTAGAACAAGTCGCAGATAAGTATCCAGCTCAA
    CAAGCAGGATTACAAAAAGGTGATAAGATCGTCCAAATTGGCAAATATAAAATATCTGAA
    TTTGATGATGTTGATAAGGCGTTAGATAAAGTTAAAGATAATAAGACGACTGTTAAATTT
    GAACGTGATGGTAAAACAAAGTCAGTTGAATTAACACCTAAAAAGACTGAAAAAAAACTG
    ACTAAAGTAAGTTCAGAGACGAAGTATGTTCTCGGATTCCAACCAGCGAGTGAACATACA
    CTTTTTAAACCAATTGTATTCGGATTTAAAAGCTTTTTAATCGGTAGTACTTATATTTTT
    ACAGCTGTAGTAGGTATGTTGGCTAGTATATTTACGGGCGGATTCTCATTTGATATGTTA
    AATGGTCCGGTTGGTATTTATCATAACGTCGACTCAGTTGTTAAAGCGGGTATCATTAGC
    TTAATTGGTTACACTGCGTTATTAAGTGTAAACTTAGGTATTATGAATTTAATTCCTATT
    CCTGCACTAGACGGTGGTCGTATTTTATTTGTTATATATGAAGCGATTTTCAGAAAACCA
    GTTAATAAAAAAGCGGAAACAACGATTATTGCTATTGGTGCCATTTTCATGGTCGTTATA
    ATGATATTAGTAACGTGGAATGATATTCGACGATATTTCTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1185

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01239
  • symbol: SAOUHSC_01239
  • description: hypothetical protein
  • length: 394
  • theoretical pI: 9.49068
  • theoretical MW: 44279.5
  • GRAVY: 0.0705584

Function[edit source | edit]

  • reaction:
    EC 3.4.24.-?  ExPASy
  • TIGRFAM:
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesRIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 236.6)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidespeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 38)
    Genetic information processingProtein fateProtein folding and stabilizationpeptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 38)
    Cellular processesCellular processesSporulation and germinationstage IV sporulation protein B (TIGR02860; EC 3.4.21.116; HMM-score: 29.7)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesC-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 27.2)
    Genetic information processingProtein fateProtein modification and repairC-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 27.2)
    putative carboxyl-terminal-processing protease, deltaproteobacterial (TIGR03900; EC 3.4.21.-; HMM-score: 19.4)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype II secretion system protein C (TIGR01713; HMM-score: 16)
  • TheSEED:  
    Regulation and Cell signalingRegulation and Cell signaling - no subcategorySex pheromones in Enterococcus faecalis and other Firmicutes Membrane-associated zinc metalloprotease 
    Stress ResponsePeriplasmic StressPeriplasmic Stress Response Membrane-associated zinc metalloprotease 
  • PFAM:
    Peptidase_MA (CL0126) Peptidase_M50; Peptidase family M50 (PF02163; HMM-score: 207.9)
    PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 44.4)
    PDZ; PDZ domain (Also known as DHR or GLGF) (PF00595; HMM-score: 23.5)
    Tricorn_PDZ; Tricorn protease PDZ domain (PF14685; HMM-score: 13.5)
    Yip1 (CL0112) DUF1129; Protein of unknown function (DUF1129) (PF06570; HMM-score: 10.5)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.12
    • Ymax_pos: 38
    • Cmax: 0.114
    • Cmax_pos: 38
    • Smax: 0.165
    • Smax_pos: 36
    • Smean: 0.1
    • D: 0.112
  • predicted transmembrane helices (TMHMM): 4

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MCPEFAIGMGPKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILDDHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRDRQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGLQKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTKVSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDMLNGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFRKPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]