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Pangenome04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01547
  • pan locus tag?: SAUPAN001631000
  • symbol: SAOUHSC_01547
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01547
  • symbol: SAOUHSC_01547
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1491472..1493469
  • length: 1998
  • essential: no DEG

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTTAGACAAAGTCACTCAAATAGAAACAATTAAATATGATCGTGATGTCTCATATTCT
    TATGCTGCTAGTCGTTTATCTACACATTGGACTAATCACAATATGGCTTGGTCTGACTTT
    ATGCAGAAGCTAGCACAAACAGTTAGAACTAAAGAAGATTTAACTGAGTACAATAAAATG
    TCTAAGTCTGAACAAGCCGATATAAAAGATGTTGGCGGATTTGTCGGTGGATATTTAAAA
    GAAGGCAAACGGCGTGCTGGTCAAGTCATGAATCGTTCAATGCTAACACTTGATATCGAT
    TATGCAGCCCAAGATATGACTGACATATTATCTATGTTTTATGATTTTGCATATTGTTTA
    TATTCAACACATAAGCATAGAGAGATAAGTCCAAGACTGCGTTTAGTGATTCCTTTAAAA
    CGAAATGTAAATGCAGATGAGTATGAAGCTATTGGGCGTAAAGTCGCAGATATCGTTGGC
    ATGGATTACTTCGATGATACAACTTATCAACCACATAGGTTAATGTATTGGCCTTCAACT
    AGTAACGATGCGGAATTTTTCTTTACCTATGAAGATTTACCTTTGTTAGACCCAGATAAA
    ATATTAAATGAATATGTTGATTGGACTGACACATTAGAATGGCCAACGTCTTCAAGGGAA
    GAGAGTAAGACTAAAAGATTAGCAGATAAGCAAGGCGACCCAGAAGAAAAGCCGGGAATT
    GTTGGTGCATTTTGTAGAGCCTATACGATAGAAGAAGCTATAGAAACTTTTATTCCTGAT
    TTATACGAAAAACATTCTACTAACCGTTATACCTATCATGAAGGTTCAACTGCAGGTGGA
    TTGGTGTTATACGAAAATAACAAGTTTGCCTATTCTCATCATAATACGGATCCCGTAAGC
    GGTATGCTTGTGAACAGTTTTGATTTAGTACGCATACACTTATATGGTGCTCAAGATGAA
    GAAACTAAAACAGATACTCCGGTTAATCGACTACCTAGTTATAAAGCAATGCAGCAAAGA
    GCGCAAAATGATGAGGTTGTTAAAAAGCAATTAATTAATGACAAAATGTCTGATGCAATG
    CAGGATTTCGATGAAATAGAAAATAGCGATGATGCATGGTCTGAGACGTTAGAAATTACT
    TCGAAAGGTACTTTCAAAGCTAGTATCCCAAATATAGAAATTATATTGCGTAATGATCCA
    AATTTAAAAGGAAAAATAGCCTTTAACGAATTTACGAAACAAATTGAATGTTTAGGGAAA
    GTGCCATGGAATACTAATTTTAAGACACGTCAATGGCAAGACGGTGATGATAGCAGTTTA
    AGAAGTTATATCGAAAAGATTTATGACATACACCATTCAGGTAAAACAAAAGATGCCATT
    ATAAGCGTAGCAATGCAAAATGCTTATCATCCAGTAAGGGATTATCTAAATAAAATATCG
    TGGGATGGACATAAACGCCTTGAAAAGTTATTTATCAAATACTTAGGTGTTGAAGACACT
    GAAGTGAATAGAACAACTACCAAAAAAGCATTGACTGCTGGAATCGCTAGAGTAATGGAG
    CCTGGATGTAAATTTGACTATATGCTTACACTTTATGGTCCTCAAGGTGTAGGTAAATCT
    GCTTTGCTAAAAAAATTAGGTGGTGCATGGTTTTCTGACAGTTTAGTTTCTGTTACAGGT
    AAAGAAGCTTATGAGGCCTTACAAGGCGTTTGGCTAATGGAAATGGCAGAACTTGCAGCT
    ACAAGAAAAGCTGAAGTTGAAGCTATTAAGCATTTCATATCTAAACAAGTTGACCGATTT
    CGTGTTGCTTATGGGCATTATATTGAAGATTTTCCAAGGCAATGTATTTTCATTGGTACA
    ACTAATAAAGTTGATTTCTTAAGAGATGAAACTGGTGGAAGACGTTTTTGGCCAATGACT
    GTAAATCCAGAGAGAGTTGAAGTGAACTGGTCTAAACTAACCAAAGATGAGATTGACCAA
    ATTTGGGCAGAAGGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    1998

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01547
  • symbol: SAOUHSC_01547
  • description: hypothetical protein
  • length: 665
  • theoretical pI: 5.47579
  • theoretical MW: 76679.9
  • GRAVY: -0.609624

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    DNA primase, phage associated 
  • PFAM:
    P-loop_NTPase (CL0023) VirE; Virulence-associated protein E (PF05272; HMM-score: 284.8)
    AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 19.9)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.9)
    ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 13.3)
    AAA_22; AAA domain (PF13401; HMM-score: 13.2)
    NACHT; NACHT domain (PF05729; HMM-score: 12.4)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 12.2)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 10.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.151
    • Ymax_pos: 39
    • Cmax: 0.213
    • Cmax_pos: 39
    • Smax: 0.167
    • Smax_pos: 35
    • Smean: 0.103
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLDKVTQIETIKYDRDVSYSYAASRLSTHWTNHNMAWSDFMQKLAQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYAAQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVGMDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSSREESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAIETFIPDLYEKHSTNRYTYHEGSTAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEETKTDTPVNRLPSYKAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIENSDDAWSETLEITSKGTFKASIPNIEIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDIHHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTKKALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEALQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKVDFLRDETGGRRFWPMTVNPERVEVNWSKLTKDEIDQIWAEG

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]