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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01612
  • pan locus tag?: SAUPAN004071000
  • symbol: SAOUHSC_01612
  • pan gene symbol?: bfmBAB
  • synonym:
  • product: 2-oxoisovalerate dehydrogenase, E1 component subunit beta

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01612
  • symbol: SAOUHSC_01612
  • product: 2-oxoisovalerate dehydrogenase, E1 component subunit beta
  • replicon: chromosome
  • strand: -
  • coordinates: 1535924..1536907
  • length: 984
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGCTAAATTATCTTATTTAGAGGCGATACGCCAGGCACAAGACTTAGCATTACAACAA
    AATAAAGATGTGTTTATTCTTGGTGAAGATGTTGGTAGAAAAGGTGGCGTATTTGGTACT
    ACTCAAGGACTGCAACAAAAATATGGTGAAGACAGAGTTATCGATACGCCATTAGCTGAG
    TCAAATATTGTTGGAACTGCCATTGGTGCAGCTATGGTAGGTAAAAGACCAATTGCAGAA
    ATTCAATTTGCAGATTTTATTTTACCTGCGACAAATCAAATTATTAGTGAAGCTGCGAAA
    ATGCGTTATCGTTCAAATAATGATTGGCAATGCCCATTAACGATTCGTGCACCATTTGGT
    GGCGGTGTTCACGGAGGACTATATCATTCACAAAGTATTGAAAGTATATTTGCTTCATCG
    CCAGGTTTAACAATTGTTATTCCTTCCACACCGTATGATGCTAAAGGGCTTTTATTATCA
    TCTATCGAATCAAATGATCCAGTATTGTACTTTGAGCATAAAAAAGCTTATCGTTTCTTA
    AAGGAAGAAGTGCCAGAAGAATATTACACTGTACCTTTAGGAAAAGCAGATGTGAAACGT
    GAAGGTGAAGATTTAACAGTATTTTGTTATGGATTAATGGTCAATTATTGTTTACAAGCA
    GCGGATATTTTAGCTGCAGACGGTATCAATGTTGAAGTCGTTGATTTACGAACTGTTTAT
    CCATTAGATAAAGAAACAATTATTGATCGTGCTAAACATACAGGTAAAGTTTTACTTGTA
    ACTGAAGATAATTTAGAAGGTAGTATTATGTCAGAAGTGTCAGCGATTATTGCAGAGCAT
    TGCTTGTTCGATTTAGATGCACCAATCATGCGTTTAGCTGCTCCAGATGTACCATCTATG
    CCATTTTCTCCTGTATTAGAAAATGAAATTATGATGAATCCAGAAAAAATCTTAAATAAA
    ATGCGTGAATTAGCAGAATTCTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    984

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01612
  • symbol: SAOUHSC_01612
  • description: 2-oxoisovalerate dehydrogenase, E1 component subunit beta
  • length: 327
  • theoretical pI: 4.52444
  • theoretical MW: 36061.2
  • GRAVY: -0.0443425

Function[edit source | edit]

  • reaction:
    EC 1.2.4.1?  ExPASy
    Pyruvate dehydrogenase (acetyl-transferring)Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2
  • TIGRFAM:
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 76.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 76.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridoxine1-deoxy-D-xylulose-5-phosphate synthase (TIGR00204; EC 2.2.1.7; HMM-score: 76.4)
    2-oxoacid:acceptor oxidoreductase, alpha subunit (TIGR03710; HMM-score: 23.1)
    indolepyruvate ferredoxin oxidoreductase, alpha subunit (TIGR03336; EC 1.2.7.8; HMM-score: 20.7)
  • TheSEED:  
    Amino Acids and DerivativesBranched-chain amino acidsIsoleucine degradation Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4) 
    Branched-chain amino acidsValine degradation Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4) 
    CarbohydratesCentral carbohydrate metabolismDehydrogenase complexes Branched-chain alpha-keto acid dehydrogenase, E1 component, beta subunit (EC 1.2.4.4) 
  • PFAM:
    THDP-binding (CL0254) Transket_pyr; Transketolase, pyrimidine binding domain (PF02779; HMM-score: 155.6)
    TKC_like (CL0591) Transketolase_C; Transketolase, C-terminal domain (PF02780; HMM-score: 135.5)
    PFOR_II; Pyruvate:ferredoxin oxidoreductase core domain II (PF17147; HMM-score: 23.5)
    no clan definedDUF2795; Protein of unknown function (DUF2795) (PF11387; HMM-score: 12.9)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.109
    • Ymax_pos: 27
    • Cmax: 0.136
    • Cmax_pos: 70
    • Smax: 0.147
    • Smax_pos: 18
    • Smean: 0.098
    • D: 0.105
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGLQQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMRYRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLSSIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCLQAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAIIAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 1.16 1.17 1.18 1.19 1.20 1.21 1.22 1.23 1.24 1.25 1.26 1.27 1.28 1.29

Relevant publications[edit source | edit]