From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01615
  • pan locus tag?: SAUPAN004074000
  • symbol: SAOUHSC_01615
  • pan gene symbol?: recN
  • synonym:
  • product: DNA repair protein RecN

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01615
  • symbol: SAOUHSC_01615
  • product: DNA repair protein RecN
  • replicon: chromosome
  • strand: -
  • coordinates: 1539487..1541166
  • length: 1680
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGTTACAAACCTTATCAATCAAGCAATTTGCTATTATTGAAGAATTAGAAATTCAATTT
    TCTGATGGTTTAACTGTTTTAAGTGGTGAAACGGGTTCAGGTAAGTCAATTATTATAGAT
    GCAATTGGACAATTAATTGGAATGAGAGCTTCATCTGATTTTGTAAGACATGGTGAAAAA
    AAGGCTGTCATCGAAGGTATATTTGATATTGACGAGAGTAAAGATGCTATACATATATTG
    AAAAATATGGATATTGATGTAGATGAAGATTTTTTATTAGTTAAACGGGAAATTTTCAGC
    TCTGGTAAAAGTCTTTGTAAAATTAATAATCAAACAGTTACTTTACAAGATTTACGAAAA
    GTTATGCAAGAATTATTAGATATTCATGGCCAACATGAAACGCAGTCTTTGTTGAAACAA
    AAATACCATTTAACATTATTGGATAATTACGCAGAATCACGTTATCAAGATTTGTTGGAT
    AAATATCACCAAACTTTTCAAAATTATAAAGCCAAAAAGCAAGAGTTAGAAGATTTAGAA
    TCAGCAGACCAAGCATTGCTACAGCGTTTAGATTTAATGAAATTCCAATTAGAAGAATTG
    TCTGAAGCACATCTAAAAGAAGGCGAAATAGAGCAACTTGAAATAGATATTAAACGCATT
    CAAAACTCTGAAAAATTAAGCTTAGCGCTTAATAACGCATATATGACATTAACGGATGAA
    AATGCTATCACTGATCGTTTATATGAACTAAGCAACCATTTGTTAACAATAAACGATATA
    GTTCCAAACAAATATGACAAATTAAAAGAAGATATTGATCAATTTTACTACATTTTAGAA
    GATGCAAAACATGAGTTATATGATGAAATGGCTAATACAGAATTCGACGAACAAGTGTTA
    AACGAGTATGAATCTCGTATGAATTTGCTGAATAATTTAAAACGTAAATATGGAAAAGAT
    ATTTCAGAATTAATCGCATATCAAGAAAAGCTTAATAATGAAATCAATAAAATTGAAAAC
    TATGAACAAAGTACATCGCAGTTACGAGAAGAAATAAATGCATTGTATAATCAAGTTATA
    GAGGTTGGACAAGCGTTATCAAAGCAGCGTCGTATAGTCGCTAGGGAGTTAAGAGACCAC
    ATTGTATCGGAAATTCAAAACTTACAAATGAAAGACGCAAATCTTGAGATTTCATTTAAA
    AAATTAGAAGAACCGAATATTGATGGAATCGAATTTGTAGAATTTTTAATCAGTCCAAAT
    AAAGGGGAACCATTAAAAAGTTTAAATAAAATTGCGTCAGGTGGAGAACTTTCTAGAATT
    ATGTTAGCGTTAAAAAGTATTTTTGTTAAATCGAGAGGTCAAACTGCAATTTTATTCGAT
    GAGGTTGACTCAGGTGTATCTGGACAAGCTGCACAAAAAATGGCAGAGAAAATGCGAGAT
    ATAGCAGAATATATACAAGTTATTTGTATCTCTCACTTACCACAAGTTGCATCGATGAGC
    GACCATCATTTATTAATTTCGAAATCATCAAAAGATGATCGTACAACAACACAAGTTCAA
    GAGTTAATTAGCGATGATAAAGTAGATGAAATTGCACGAATGATTTCAGGTGCAAGTGTT
    ACTGATTTAACTCGAGAAAATGCAAGAGAAATGATACAACACAATCAAAGACGTAGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01615
  • symbol: SAOUHSC_01615
  • description: DNA repair protein RecN
  • length: 559
  • theoretical pI: 4.70128
  • theoretical MW: 64321.5
  • GRAVY: -0.498748

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA repair protein RecN (TIGR00634; HMM-score: 547)
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02169; HMM-score: 45.6)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02169; HMM-score: 45.6)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA replication and repair protein RecF (TIGR00611; HMM-score: 28.7)
    thiol reductant ABC exporter, CydD subunit (TIGR02857; HMM-score: 23)
    Cellular processesCellular processesCell divisionchromosome segregation protein SMC (TIGR02168; HMM-score: 20.5)
    Genetic information processingDNA metabolismChromosome-associated proteinschromosome segregation protein SMC (TIGR02168; HMM-score: 20.5)
    MetabolismTransport and binding proteinsUnknown substrateenergy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 19)
    phosphonate C-P lyase system protein PhnL (TIGR02324; HMM-score: 13.8)
    MetabolismTransport and binding proteinsAmino acids, peptides and aminesurea ABC transporter, ATP-binding protein UrtD (TIGR03411; HMM-score: 8.5)
    MetabolismTransport and binding proteinsCations and iron carrying compoundspotassium uptake protein (TIGR00794; HMM-score: 6.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMolybdopterinMoaD family protein (TIGR01687; HMM-score: 5.9)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype I secretion membrane fusion protein, HlyD family (TIGR01843; HMM-score: 3.5)
  • TheSEED:  
    DNA MetabolismDNA repairDNA repair, bacterial DNA repair protein RecN 
    DNA replicationDNA-replication DNA repair protein RecN 
  • PFAM:
    P-loop_NTPase (CL0023) SMC_N; RecF/RecN/SMC N terminal domain (PF02463; HMM-score: 75.7)
    AAA_23; AAA domain (PF13476; HMM-score: 52.1)
    AAA_15; AAA ATPase domain (PF13175; HMM-score: 27.2)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 24.5)
    AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 23.2)
    AAA_16; AAA ATPase domain (PF13191; HMM-score: 15.3)
    AAA_22; AAA domain (PF13401; HMM-score: 13.7)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 13.3)
    GH57_38_middle (CL0599) DUF1957; Domain of unknown function (DUF1957) (PF09210; HMM-score: 11)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.113
    • Ymax_pos: 24
    • Cmax: 0.126
    • Cmax_pos: 51
    • Smax: 0.139
    • Smax_pos: 18
    • Smean: 0.107
    • D: 0.11
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQLIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSGKSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLDKYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIKRIQNSEKLSLALNNAYMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFYYILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELIAYQEKLNNEINKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKDANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVKSRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISKSSKDDRTTTQVQELISDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01617 (ArgR*)
    ArgR* (TF) important in Arginine biosynthesis, Arginine degradationRegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]