From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01649
  • pan locus tag?: SAUPAN004129000
  • symbol: SAOUHSC_01649
  • pan gene symbol?:
  • synonym:
  • product:

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01649
  • symbol: SAOUHSC_01649
  • product:
  • replicon: chromosome
  • strand: -
  • coordinates: 1562458..1563921
  • length: 1464
  • essential: no [1] DEG other strains
  • comment: The sequence of SAOUHSC_01649 was corrected based on the resequencing performed by Berscheid et al., 2012 [2].

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAACATAGACAAACAATTTTGGAAAACAATATATTATTGGATTAGGTACTTGAATTTT
    GATATTGTCAGTAGGGAAAAAGATGACCAAGAAATTTGGTTAGCCCATAAAAGAAAAAAA
    CAAGTAGTCATTTTTAAACAACATATCAAGTCTACCCAAGAAATACGTTTTGACAAAGCG
    AAAGTGCTTGAACACAAAGATGAAATAGCAAATTTTATTTCTTTCGAACCACAAAGTTTT
    GAATTTTATTATTTTACAGAATCGGAATTTTCAGAAGAACAATTAAATGAAGTTTCGCCA
    ATTAGAATTAAATTCAATGTTATAAGACACACAAAAGATTTGATAAAGCATATGCCGAAT
    ATATTTTTGGCTAGACTTATTTCAGAAGATAATGATAAAAAGACATATATGTTCTATAAA
    CGCAAAGTATTAACCGATAACTTTTTAGATAAATATATGCAGAAATTTTCACCGGCAACA
    TACACAATAATATTTGTAAATGTCTTAATATGGTTATGTATGATTTTATATTTAAATAAT
    TTTTCGGATGTAAAATTATTAGATGTTGGCGGGTTAGTGCATTTTAATGTCGTACATGGT
    GAATGGTATCGAATTGTTACATCGATGTTTTTACATTTTAGTTTTGAACATATACTTATG
    AATATGCTTTCATTATTTATTTTTGGTAAAATAGTCGAAGCAATTATTGGTTCATGGCGG
    ATGTTAACTGTATACTTTATTGCAGGGTTGTTTGGAAACTTTGTATCACTATCATTTAAT
    ACGACTACAATTTCAGTTGGGGCTAGTGGTGCTATATTTGGTCTGATTGGATCAATTTTT
    GCGATGATGTATGTTTCAAAAACATTTAACAAAAAAATGTTAGGACAGTTATTAATTGCA
    TTAGTGATATTAGTTGGTGTTTCTCTGTTTATGTCAAATATAAATATTGTGGCGCATATT
    GGAGGATTCATTGGTGGTTTATTAATAACTTTAATTGGCTATTACTATAAAGTGAATCGT
    AATATTTTTTGGATTTTACTAATTGGTATGCTTGTTATATTTATTGCACTTCAAATTAGA
    ATTTTTACAATTAAAGAAGATAATATTTATAATAAATTGATCAAAGATGATATGACTAGT
    GGTAATTATGATAATGCTCAAAATATTGCGAAGCAGACAATAAATAAAAATTATGCCGAT
    GATCAAACATATTATTTAAGTGGTATGATTATGGCTACTATCAATTCTAAGTCCGAAGGT
    ATGACAGAATGGGAGCGAGGACTTAGAATGTTTCCTAAATCAGGTTTATTAAATTTTGAG
    TTAGCGATAGCAAATCGTTCATTAAATGATGATGAAAAAGCATTAAAATATGTGCGTAAA
    GCATTAAATGCAGACCCTAAAAATACAGATTATATTAACTTAGAAAAAGAGTTGACTAAA
    TCAAATGAGTCGAAAAATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1464

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01649
  • symbol: SAOUHSC_01649
  • description:
  • length: 487
  • theoretical pI: 9.54533
  • theoretical MW: 56861.1
  • GRAVY: 0.0519507

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    rhomboid family protease GlpG (TIGR04239; EC 3.4.21.105; HMM-score: 54.7)
    rhombosortase (TIGR03902; EC 3.4.21.-; HMM-score: 42.9)
    putative PEP-CTERM system TPR-repeat lipoprotein (TIGR02917; HMM-score: 20.8)
    type IV pilus biogenesis/stability protein PilW (TIGR02521; HMM-score: 15.6)
  • TheSEED:  
    FIG056164: rhomboid family serine protease 
  • PFAM:
    Rhomboid-like (CL0207) Rhomboid; Rhomboid family (PF01694; HMM-score: 119.3)
    TPR (CL0020) TPR_2; Tetratricopeptide repeat (PF07719; HMM-score: 20.4)
    Fis1_TPR_C; Fis1 C-terminal tetratricopeptide repeat (PF14853; HMM-score: 19)
    TPR_8; Tetratricopeptide repeat (PF13181; HMM-score: 17.2)
    TPR_16; Tetratricopeptide repeat (PF13432; HMM-score: 15.7)
    TPR_19; Tetratricopeptide repeat (PF14559; HMM-score: 14.8)
    TPR_1; Tetratricopeptide repeat (PF00515; HMM-score: 13.7)
    TPR_12; Tetratricopeptide repeat (PF13424; HMM-score: 12.1)
    no clan definedSensor_TM1; Sensor N-terminal transmembrane domain (PF13755; HMM-score: 10.2)
    O-anti_assembly (CL0499) Wzy_C; O-Antigen ligase (PF04932; HMM-score: 5.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 7
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.095
    • Ymax_pos: 70
    • Cmax: 0.107
    • Cmax_pos: 29
    • Smax: 0.101
    • Smax_pos: 58
    • Smean: 0.067
    • D: 0.084
  • predicted transmembrane helices (TMHMM): 7

Accession numbers[edit source | edit]

  • GI:
  • RefSeq:
  • UniProt: Q2FY24 UniProt

Protein sequence[edit source | edit]

  • MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVIFKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIRIKFNVIRHTKDLIKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLDKYMQKFSPATYTIIFVNVLIWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHFSFEHILMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGAIFGLIGSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLITLIGYYYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQNIAKQTINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIANRSLNDDEKALKYVRKALNADPKNTDYINLEKELTKSNESKNK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
    Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
    Int. J. Med. Microbiol.: 2012, 302(2);84-7
    [PubMed:22417616] [WorldCat.org] [DOI] (I p)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]