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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01700
  • pan locus tag?: SAUPAN004183000
  • symbol: SAOUHSC_01700
  • pan gene symbol?:
  • synonym:
  • product: GTP-binding protein YqeH

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01700
  • symbol: SAOUHSC_01700
  • product: GTP-binding protein YqeH
  • replicon: chromosome
  • strand: -
  • coordinates: 1608251..1609351
  • length: 1101
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGTCTGACATTTTAAAATGTATCGGTTGTGGTGCGCCACTTCAATCTGAAGATAAAAAT
    AAACCTGGTTTTGTACCAGAGCATAATATGTTTCGTGATGACGTGATTTGCAGACGTTGT
    TTCCGCTTGAAAAATTATAACGAAGTTCAAGATGTAGGATTAGAAAGTGAAGACTTTTTA
    AAATTATTATCAGGACTTGCGGATAAAAAGGGTATTGTCGTCAATGTCGTGGATGTATTT
    GACTTTGAAGGATCATTTATTAATGCAGTTAAACGTATTGTCGGAAACAAAAAAATCATT
    TTAGCAGCAAATAAATTGGATTTATTGCCGAAACAAATTAATAAACGTCGAGTTAAAGAA
    TGGTTAAAACGAACAGCAAGAAAATATGGTTTGGAAGCTGACGATGTCGTATTAATTTCA
    GCTGAAAAAGGCTGGGGCATAGACGACTTATTATCATCAATTGCGAATATTCGAGAAAAT
    GAAGATGTGTATATTGTAGGGACAACGAATGTTGGGAAATCTACATTGATTAATAAACTG
    ATTGAAGCTAGTGTTGGTGAAAAAGATGTAGTAACAACTTCAAGATTCCCTGGAACAACT
    TTAGATATGATAGATATTCCTTTAGATGAAACATCATTTATGTATGATACACCAGGTATT
    ATTCAAGATCACCAAATGACGCATTTAGTTAGTGAAAAAGAATTGAAAATTATTATGCCT
    AAGAAAGAAATAAAACAACGCGTATATCAATTAAATGAGGCGCAGACATTATTCTTCGGC
    GGTCTAGCGCGCATAGATTATGTATCAGGTGGTAAACGTCCGTTAGTTTGTTTCTTTTCT
    AATGACTTGAATATACATCGTACTAAAACGGAGAAGGCTAATGATTTATGGCGTAATCAA
    CTTGGCGATTTATTAACGCCACCTGGAAATCCACAAAATTTTGATCTTAATGAGGTAAAG
    GCTGTTAGACTTGAAACAGGCAAAGAGAAACGCGATGTTATGATCTCTGGTCTAGGCTTT
    ATAACTATAGGACCAGGGGCTAAAGTAATCGTTCGTGTTCCTAAAAATGTTGATGTTGTA
    TTAAGAAATTCTATTTTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1101

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01700
  • symbol: SAOUHSC_01700
  • description: GTP-binding protein YqeH
  • length: 366
  • theoretical pI: 8.44888
  • theoretical MW: 41086.3
  • GRAVY: -0.22459

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesisOtherribosome biogenesis GTPase YqeH (TIGR03597; HMM-score: 486.9)
    Genetic information processingProtein synthesisOtherribosome-associated GTPase EngA (TIGR03594; HMM-score: 60.6)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTP-binding protein YlqF (TIGR03596; HMM-score: 52.5)
    Genetic information processingProtein synthesisOtherribosome biogenesis GTP-binding protein YsxC (TIGR03598; HMM-score: 40)
    Unknown functionGeneralGTP-binding protein HflX (TIGR03156; HMM-score: 37.5)
    Unknown functionGeneralsmall GTP-binding protein domain (TIGR00231; HMM-score: 37.4)
    Genetic information processingProtein synthesisOtherGTP-binding protein Era (TIGR00436; HMM-score: 33.2)
    Genetic information processingProtein synthesisOtherObg family GTPase CgtA (TIGR02729; HMM-score: 32.9)
    Genetic information processingProtein fateProtein modification and repair[FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 30.5)
    Genetic information processingProtein synthesisTranslation factorsribosome small subunit-dependent GTPase A (TIGR00157; EC 3.6.-.-; HMM-score: 28.5)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA modification GTPase TrmE (TIGR00450; EC 3.6.-.-; HMM-score: 28.2)
    MetabolismTransport and binding proteinsCations and iron carrying compoundsferrous iron transport protein B (TIGR00437; HMM-score: 22.9)
    Genetic information processingProtein synthesisTranslation factorsselenocysteine-specific translation elongation factor (TIGR00475; HMM-score: 21.9)
    Unknown functionGeneralelongation factor 4 (TIGR01393; EC 3.6.5.-; HMM-score: 18.5)
    translation initiation factor 2, gamma subunit (TIGR03680; HMM-score: 18.4)
    MetabolismEnergy metabolismAmino acids and aminesethanolamine utilization protein, EutP (TIGR02528; HMM-score: 15.3)
    Genetic information processingProtein synthesisTranslation factorstranslation initiation factor IF-2 (TIGR00487; HMM-score: 14.2)
    Unknown functionGeneralGTP-binding protein YchF (TIGR00092; HMM-score: 13.9)
    MetabolismTransport and binding proteinsUnknown substrateenergy-coupling factor transporter ATPase (TIGR04521; EC 3.6.3.-; HMM-score: 11.7)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype VII secretion protein EccCb (TIGR03925; HMM-score: 11)
  • TheSEED:  
    Protein MetabolismProtein biosynthesisUniversal GTPases GTP-binding protein YqeH, required for biogenesis of 30S ribosome subunit 
  • PFAM:
    P-loop_NTPase (CL0023) MMR_HSR1; 50S ribosome-binding GTPase (PF01926; HMM-score: 54.2)
    RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 39.8)
    FeoB_N; Ferrous iron transport protein B (PF02421; HMM-score: 34.6)
    GTP_EFTU; Elongation factor Tu GTP binding domain (PF00009; HMM-score: 27.3)
    Roc; Ras of Complex, Roc, domain of DAPkinase (PF08477; HMM-score: 21.9)
    Ras; Ras family (PF00071; HMM-score: 19.6)
    Dynamin_N; Dynamin family (PF00350; HMM-score: 18.8)
    ABC_tran; ABC transporter (PF00005; HMM-score: 17.2)
    no clan definedMMR_HSR1_Xtn; C-terminal region of MMR_HSR1 domain (PF16897; HMM-score: 17)
    P-loop_NTPase (CL0023) ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 16.3)
    RNA_helicase; RNA helicase (PF00910; HMM-score: 14.4)
    ArgK; ArgK protein (PF03308; HMM-score: 13.4)
    AAA_18; AAA domain (PF13238; HMM-score: 12.8)
    PduV-EutP; Ethanolamine utilisation - propanediol utilisation (PF10662; HMM-score: 12.4)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 12.3)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 12.2)
    no clan definedSwi5; Swi5 (PF07061; HMM-score: 12.2)
    P-loop_NTPase (CL0023) FtsK_SpoIIIE; FtsK/SpoIIIE family (PF01580; HMM-score: 12.1)
    Arf; ADP-ribosylation factor family (PF00025; HMM-score: 8.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.131
    • Ymax_pos: 22
    • Cmax: 0.106
    • Cmax_pos: 22
    • Smax: 0.201
    • Smax_pos: 15
    • Smean: 0.134
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLKNYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILAANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIRENEDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTPGIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLVCFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVMISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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