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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01722
  • pan locus tag?: SAUPAN004215000
  • symbol: alaS
  • pan gene symbol?: alaS
  • synonym:
  • product: alanyl-tRNA synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01722
  • symbol: alaS
  • product: alanyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1625259..1627889
  • length: 2631
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGAAAAAGTTAAAAGCGAGTGAAATTAGACAAAAATATCTAGATTTCTTTGTTGAAAAA
    GGACATATGGTTGAACCTTCTGCACCATTAGTGCCAATTGATGATGATACATTATTATGG
    ATTAATTCAGGTGTAGCAACATTAAAGAAATATTTTGATGGACGTGAAACACCTAAAAAG
    CCAAGAATTGTAAACTCTCAAAAAGCTATTCGTACAAATGATATTGAAAATGTTGGTTTC
    ACAGCGCGTCACCATACATTCTTTGAAATGTTAGGTAACTTCTCAATTGGTGATTATTTT
    AAACAAGAAGCGATTGAATTTGCTTGGGAATTTTTAACGAGTGATAAATGGATGGGTATG
    GAGCCAGATAAATTGTACGTTACGATTCATCCGGAAGATATGGAAGCATACAACATTTGG
    CATAAAGATATTGGGCTTGAAGAAAGTCGTATTATTCGCATTGAAGGTAACTTCTGGGAT
    ATTGGTGAAGGGCCTTCAGGACCGAACACTGAGATTTTCTATGATCGCGGAGAAGCATAT
    GGACAAGACGATCCGGCAGAAGAAATGTATCCAGGTGGAGAAAATGAACGCTATCTTGAA
    GTATGGAACTTAGTATTTAGTGAATTCAATCATAATAAAGATCATAGTTACACACCATTA
    CCTAATAAAAATATTGATACTGGCATGGGGCTTGAGCGTATGGCCTCAGTTTCTCAAAAT
    GTACGTACTAACTATGAAACAGATTTATTTATGCCTATAATGAATGAAATCGAAAAAGTA
    TCAGGTAAACAATATTTAGTAAACAACGAACAAGATGTGGCATTTAAAGTAATTGCTGAC
    CACATTCGTACGATTGCATTTGCAATTTCTGATGGTGCATTACCTGCCAATGAAGGTAGA
    GGGTATGTATTACGTCGATTGTTACGTCGTGCCGTTCGTTTTAGTCAAACGTTAGGAATC
    AATGAGCCATTTATGTACAAACTTGTTGATATTGTTGCAGACATTATGGAACCATATTAT
    CCAAATGTTAAGGAAAAAGCAGATTTCATTAAGCGTGTTATAAAGTCTGAAGAAGAACGA
    TTCCATGAAACATTAGAAGATGGTTTAGCGATTTTAAATGAATTAATTAAAAAAGCTAAA
    GCGACAACAAATGAAATTAATGGGAAAGATGCATTTAAATTGTATGATACGTATGGGTTC
    CCAATTGAATTAACTGAAGAAATAGCAGTGCAAGCAGGATTGAAAGTTGATATGACAACA
    TTCGAGTCAGAAATGCAACAACAACGTGATCGTGCACGTCAAGCACGTCAAAATTCTCAA
    TCAATGCAAGTTCAAAGTGAAGTATTGAAAAATATTACATCTGCAAGTACTTTTGTTGGT
    TATGATACTGCGACAGCTCAAACAACACTAACACACTTGATATACAATGGTGAAGAAGTT
    TCACAAGTTGAAGCGGGTGAAACAGTATACTTCATGTTAACGGAAACACCATTTTATGCA
    ATCAGTGGTGGACAAGTTGCGGATACAGGTATTGTTTATAATGACAATTTTGAAATTGCT
    GTTAGTGAAGTAACCAAAGCACCAAATGGTCAAAACTTGCATAAAGGAGTAGTACAATTT
    GGCCAAGTAAATGTTGGCGCTACAGTGTCTGCTGAAGTGAACCAAAATGATCGACGTGAC
    ATTCAAAAGAACCATAGTGCAACACATTTATTACATGCAGCGTTGAAATCAGTACTGGGT
    GATCATGTTAACCAAGCTGGTTCACTAGTAGAAGCAGATCGTTTACGTTTTGATTTCTCT
    CATTTTGGTCCAATGACTAATGATGAAATTGATCAAGTTGAACGCTTAGTAAATGAAGAA
    ATTTGGAAAGGTATTGACGTTAACATTCAAGAAATGGATATTGCTTCAGCTAAAGAAATG
    GGCGCAATGGCATTATTCGGTGAAAAATATGGTGATGTTGTGCGTGTAGTAAATATGGCA
    CCATTTTCAATTGAATTATGTGGTGGTATTCATGTCCGCAATACTTCTGAAATTGGCTTA
    TTCAAAATAGTAAGTGAGTCAGGTACAGGAGCTGGTGTGCGTCGTATTGAAGCATTAACA
    GGTAAAGCAGCTTTCTTATATTTAGAAGATATTCAAGAGAAATTTAATACGATGAAATCA
    CAGCTGAAAGTGAAATCTGATGATCAAGTAGTCGATAAGTTAACACAATTACAAGATGAA
    GAAAAAGCATTATTAAAACAATTAGAGCAACGTGACAAAGAAATCACATCACTTAAAATG
    GGTAATATTGAAGATCAAGTTGAAGAAATCAATGGCTATAAAGTATTGGTTACTGAAGTG
    GATGTACCAAATGCGAAAGCAATTCGCTCGACAATGGACGATTTTAAATCTAAACTACAA
    GATACAATTATCATTCTTGCAAGTAATGTTGATGATAAAGTATCGATGGTTGCAACTGTC
    CCTAAATCTTTAACAAATAACGTTAAAGCCGGTGATCTTATCAAACAAATGGCACCAATC
    GTTGGTGGTAAAGGTGGCGGTCGTCCAGATATGGCTCAAGGTGGCGGTACACAACCTGAA
    AATATCTCAAAATCATTAAGCTTTATTAAAGATTACATTAAAAATCTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2631

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01722
  • symbol: AlaS
  • description: alanyl-tRNA synthetase
  • length: 876
  • theoretical pI: 4.75077
  • theoretical MW: 98519.6
  • GRAVY: -0.418379

Function[edit source | edit]

  • reaction:
    EC 6.1.1.7?  ExPASy
    Alanine--tRNA ligaseATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationalanine--tRNA ligase (TIGR00344; EC 6.1.1.7; HMM-score: 1065.1)
    alanine--tRNA ligase (TIGR03683; EC 6.1.1.7; HMM-score: 421.9)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesclan AA aspartic protease, TIGR02281 family (TIGR02281; EC 3.4.23.-; HMM-score: 12.1)
  • TheSEED:  
    Protein MetabolismProtein biosynthesistRNA aminoacylation, Ala Alanyl-tRNA synthetase (EC 6.1.1.7) 
    CBSS-257314.1.peg.488 Alanyl-tRNA synthetase (EC 6.1.1.7) 
    CBSS-281090.3.peg.464 Alanyl-tRNA synthetase (EC 6.1.1.7) 
  • PFAM:
    tRNA_synt_II (CL0040) tRNA-synt_2c; tRNA synthetases class II (A) (PF01411; HMM-score: 696.1)
    no clan definedDHHA1; DHHA1 domain (PF02272; HMM-score: 107.2)
    Peptidase_ME (CL0094) tRNA_SAD; Threonyl and Alanyl tRNA synthetase second additional domain (PF07973; HMM-score: 65)
    no clan definedSwm2; Nucleolar protein Swm2 (PF17083; HMM-score: 14.8)
    FAM76; FAM76 protein (PF16046; HMM-score: 9.7)
    CCDC24; Coiled-coil domain-containing protein 24 family (PF15669; HMM-score: 8.9)
    IncA; IncA protein (PF04156; HMM-score: 6.7)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.125
    • Ymax_pos: 11
    • Cmax: 0.106
    • Cmax_pos: 7
    • Smax: 0.183
    • Smax_pos: 1
    • Smean: 0.15
    • D: 0.135
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSGVATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQEAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWDIGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYTPLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNNEQDVAFKVIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVADIMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAFKLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLKNITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAISGGQVADTGIVYNDNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHSATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTNDEIDQVERLVNEEIWKGIDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFKIVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDEEKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSKLQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGGTQPENISKSLSFIKDYIKNL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  3. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  5. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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