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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01726
  • pan locus tag?: SAUPAN004221000
  • symbol: SAOUHSC_01726
  • pan gene symbol?: mnmA
  • synonym:
  • product:

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01726
  • symbol: SAOUHSC_01726
  • product:
  • replicon: chromosome
  • strand: -
  • coordinates: 1631841..1632958
  • length: 1119
  • essential: yes [1] DEG other strains
  • comment: The sequence of SAOUHSC_01726 was corrected based on the resequencing performed by Berscheid et al., 2012 [2].

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    TTGTCAAATAAAGATATACGTGTTGTCGTTGGTATGTCAGGCGGTGTAGATAGTTCTGTA
    ACAGCCCACGTCTTAAAAGAACAAGGTTATGATGTCATTGGCATATTTATGAAAAACTGG
    GATGACACTGACGAAAATGGCGTATGTACTGCAACTGAAGATTACAACGATGTTATTGAA
    GTGTGTAATCAAATTGGCATTCCGTATTACGCTGTTAATTTTGAAAAAGAATATTGGGAT
    AAAGTCTTTACGTATTTCTTAGATGAATACAAAAAAGGTCGTACTCCAAATCCAGACGTT
    ATGTGTAATAAAGAAATTAAGTTTAAAAGCCTTTTAGATCATGCGATGAATTTAGGTGCA
    GATTATGTAGCAACAGGACATTACGCACGCATACATCGTCATGAAGATGGTCATGTTGAA
    ATGTTACGTGGTGTAGATAATAATAAAGATCAAACATACTTCTTGAATCAATTATCTCAA
    CAACAACTTTCAAAAGTGATGTTCCCAATTGGCGACATCGAAAAGAGTGAAGTGCGTCGA
    ATTGCTGAAGAACAAGGACTTGTTACTGCTAAGAAAAAAGATTCTACAGGCATTTGTTTT
    ATCGGCGAAAAAAACTTTAAAACATTTTTATCACAATATTTACCTGCACAACCGGGTGAT
    ATGATAACACTTGATGGTAAGAAAATGGGTAAACATAGTGGTTTGATGTATTACACAATA
    GGACAAAGACATGGATTAGGTATAGGTGGAGATGGCGATCCTTGGTTTGTTGTCGGTAAA
    AACCTAAAAGATAATGTTTTATATGTAGAACAAGGATTCCATCACGATGCATTATACAGT
    GATTACTTAATTGCTTCAGACTATTCATTTGTAAATCCAGAAGATAATGACTTAGATCAA
    GGTTTTGAATGTACAGCTAAATTTAGATATCGCCAAAAAGATACGAAAGTTTTTGTGAAA
    CGTGAAAACGACCATGCACTACGTGTTACTTTTGCTGAGCCAGTAAGAGCAATCACACCT
    GGACAAGCAGTTGTTTTTTATCAAGGTGATGTGTGTCTTGGTGGTGCAACAATTGACGAT
    GTGTTCAAAAATGAAGGTCAATTAAATTATGTTGTATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1119

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01726
  • symbol: SAOUHSC_01726
  • description:
  • length: 372
  • theoretical pI: 5.15955
  • theoretical MW: 42132.2
  • GRAVY: -0.461559

Function[edit source | edit]

  • reaction:
    EC 2.8.1.13?  ExPASy
    tRNA-uridine 2-sulfurtransferaseA [protein]-S-sulfanyl-L-cysteine + uridine34 in tRNA + ATP + reduced acceptor = a [protein]-L-cysteine + 2-thiouridine34 in tRNA + AMP + diphosphate + acceptor
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (TIGR00420; EC 2.1.1.61; HMM-score: 453.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA(Ile)-lysidine synthetase (TIGR02432; EC 6.3.4.-; HMM-score: 25.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesNAD+ synthetase (TIGR00552; EC 6.3.1.5; HMM-score: 24.9)
    Hypothetical proteinsConservedTIGR00268 family protein (TIGR00268; HMM-score: 24.1)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotidesPurine ribonucleotide biosynthesisGMP synthase (glutamine-hydrolyzing), C-terminal domain (TIGR00884; EC 6.3.5.2; HMM-score: 18.7)
    Unknown functionGeneralqueuosine biosynthesis protein QueC (TIGR00364; HMM-score: 16.8)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 15.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminetRNA sulfurtransferase ThiI (TIGR00342; EC 2.8.1.4; HMM-score: 15.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminputative heme d1 biosynthesis radical SAM protein NirJ2 (TIGR04055; EC 1.3.99.-; HMM-score: 15.2)
    MetabolismAmino acid biosynthesisGlutamate familyargininosuccinate synthase (TIGR00032; EC 6.3.4.5; HMM-score: 14.8)
    Plasmodium falciparum CPW-WPC domain (TIGR01492; HMM-score: 12.2)
  • TheSEED:  
    Cofactors, Vitamins, Prosthetic Groups, PigmentsRiboflavin, FMN, FADRiboflavin, FMN and FAD metabolism Extended tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61) 
  • PFAM:
    HUP (CL0039) tRNA_Me_trans; tRNA methyl transferase (PF03054; HMM-score: 491)
    NAD_synthase; NAD synthase (PF02540; HMM-score: 24.7)
    QueC; Queuosine biosynthesis protein QueC (PF06508; HMM-score: 20.7)
    ATP_bind_3; PP-loop family (PF01171; HMM-score: 18.4)
    ThiI; Thiamine biosynthesis protein (ThiI) (PF02568; HMM-score: 15.6)
    Asn_synthase; Asparagine synthase (PF00733; HMM-score: 15.1)
    TIKI (CL0572) Cofac_haem_bdg; Haem-binding uptake, Tiki superfamily, ChaN (PF04187; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:
    SAOUHSC_02494(rpsE)30S ribosomal protein S5  [3] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.164
    • Ymax_pos: 23
    • Cmax: 0.137
    • Cmax_pos: 23
    • Smax: 0.307
    • Smax_pos: 21
    • Smean: 0.223
    • D: 0.187
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI:
  • RefSeq:
  • UniProt: Q2FXV6 UniProt

Protein sequence[edit source | edit]

  • MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTDENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMCNKEIKFKSLLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQQQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQPGDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHDALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEPVRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Anne Berscheid, Peter Sass, Konstantin Weber-Lassalle, Ambrose L Cheung, Gabriele Bierbaum
    Revisiting the genomes of the Staphylococcus aureus strains NCTC 8325 and RN4220.
    Int. J. Med. Microbiol.: 2012, 302(2);84-7
    [PubMed:22417616] [WorldCat.org] [DOI] (I p)
  3. Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  4. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  5. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  6. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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