From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01802
  • pan locus tag?: SAUPAN004320000
  • symbol: SAOUHSC_01802
  • pan gene symbol?: citZ
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01802
  • symbol: SAOUHSC_01802
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1702811..1703668
  • length: 858
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    GTGCATCCAATGACAGCATTACGTACGTCATTATCATATATTGCACATTTCGATCCTGAT
    GCTGAAAATGAATCAGATGAAAATCGTTATGAAAGAGCAATGCGTATACAGGCTAAAGTA
    GCATCATTAGTTACAGCGTTTGCTCGAGTAAGACAAGATAAAGAACCACTTAAGCCTAAT
    CCTGACTTAAGTTATGCGGCAAACTTCCTATATATGTTACGTGGGGAATTACCAACAGAT
    ATAGAAGTAGAAGCCTTCAATAAAGCACTTATTTTACACGCTGATCATGAGTTGAACGCA
    TCTGCATTTACGGCACGTTGTGCGGTATCATCATTGTCAGATATGTACTCAGGTATTGTA
    GCAGCCGTAGGTTCTCTGAAAGGGCCATTACATGGTGGTGCAAACGAACAAGTTATGACG
    ATGTTATCTGAGATTGGGTCAATTGAAAATGTTGATGCTTACTTAGATGAAAAATTTGCT
    AATAAAGATAAAGTAATGGGCTTCGGTCATCGTGTATATAAAGATGGTGATCCTAGAGCG
    AAATATTTAAGAGAAATGAGCCGTCAAATTACGAAAGACGCTGGTCGTGAAGAATTATTT
    GAAATGTCAGTGAAAATGGAAAAACGTATGGCAGAAGAAAAAGGATTAATTCCTAATGTT
    GATTTTTATAGTGCGAGTGTTTATCACTGTATGGAAATACCTCATGACTTATTCACGCCA
    ATCTTTGCTGTAAGTCGTTCTGCAGGATGGATTGCTCATATTTTAGAACAATATAAAGAT
    AATAGAATTATGCGTCCTAGAGCGAAATATATTGGCGAAACGAATCGTAAGTATATCCCG
    CTTGAAGAAAGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    858

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01802
  • symbol: SAOUHSC_01802
  • description: hypothetical protein
  • length: 285
  • theoretical pI: 6.61058
  • theoretical MW: 32391.8
  • GRAVY: -0.442807

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    2-methylcitrate synthase/citrate synthase II (TIGR01800; HMM-score: 401.8)
    MetabolismEnergy metabolismTCA cyclecitrate (Si)-synthase (TIGR01798; EC 2.3.3.1; HMM-score: 218.5)
    citrate (Si)-synthase, eukaryotic (TIGR01793; EC 2.3.3.1; HMM-score: 75.3)
  • TheSEED:  
    CarbohydratesCentral carbohydrate metabolismTCA Cycle Citrate synthase (si) (EC 2.3.3.1) 
    One-carbon MetabolismSerine-glyoxylate cycle Citrate synthase (si) (EC 2.3.3.1) 
    RespirationRespiration - no subcategoryBiogenesis of c-type cytochromes Citrate synthase (si) (EC 2.3.3.1) 
  • PFAM:
    no clan definedCitrate_synt; Citrate synthase, C-terminal domain (PF00285; HMM-score: 347.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.16
    • Ymax_pos: 24
    • Cmax: 0.138
    • Cmax_pos: 24
    • Smax: 0.401
    • Smax_pos: 1
    • Smean: 0.19
    • D: 0.172
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MHPMTALRTSLSYIAHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYMLRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPLHGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSRQITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSRSAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]