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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01857
  • pan locus tag?: SAUPAN004408000
  • symbol: SAOUHSC_01857
  • pan gene symbol?: ftsK
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01857
  • symbol: SAOUHSC_01857
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 1763159..1766983
  • length: 3825
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCTGGTTTGATAAATTATTCGGCGAAGATAATGATTCAAATGATGACTTGATTCAT
    AGAAAGAAAAAAAGACGTCAAGAATCACAAAATATAGATAACGATCATGACTCATTACTG
    CCTCAAAATAATGATATTTATAGTCGTCCGAGGGGAAAATTCCGTTTTCCTATGAGCGTA
    GCTTATGAAAATGAAAATGTTGAACAATCTGCAGATACTATTTCAGATGAAAAAGAACAA
    TACCATCGAGACTATCGCAAACAAAGCCACGATTCTCGTTCACAAAAACGACATCGCCGT
    AGAAGAAATCAAACAACTGAAGAACAAAATTATAGTGAACAACGTGGGAATTCTAAAATA
    TCACAGCAAAGTATAAAATATAAAGATCATTCACATTACCATACGAATAAGCCAGGTACA
    TATGTTTCTGCAATTAATGGTATTGAGAAGGAAACGCACAAGCCAAAAACACATAATATG
    TATTCTAATAATACAAATCATCGTGCTAAAGATTCAACTCCAGATTATCACAAAGAAAGT
    TTCAAGACTTCAGAGGTACCGTCAGCTATTTTTGGCACAATGAAACCTAAAAAGTTAGAA
    AATGGTCGTATCCCTGTAAGTAAACCTTCAGAAAAAGTTGAGTCAGATAAACAAAAATAT
    GATAAATATGTAGCTAAGACGCAAACGTCTCAAAATAAACAATTAGAACAAGAAAAACAA
    AATGATAGTGTTGTCAAACAAGGAACTGCATCTAAATCATCTGATGAAAATGTATCATCA
    ACAACAAAATCAATGCCTAATTATTCAAAAGTTGATAATACTATCAAAATTGAAAATATT
    TATGCTTCACAAATTGTTGAAGAAATTAGACGTGAACGAGAACGTAAAGTGCTTCAAAAG
    CGTCGATTTAAAAAAGCGTTGCAACAAAAGCGTGAAGAACATAAAAACGAAGAGCAAGAT
    GCAATACAACGTGCAATTGATGAAATGTATGCTAAACAAGCGGAACGCTATGTTGGTGAT
    AGTTCATTAAATGATGATAGTGACTTAACAGATAATAGTACAGATGCTAGTCAGCTTCAT
    ACAAATGGCATAGAGAATGAAACTGTATCAAATGATGAAAATAAACAAGCGTCAATACAA
    AATGAAGACACTAATGACACTCATGTAGATGAAAGTCCATACAATTATGAGGAAGTTAGT
    TTGAATCAAGTATCGACAACAAAACAATTGTCAGATGATGAAGTTACGGTTTCGAATGTA
    ACGTCTCAACATCAATCAGCACTACAACATAACGTTGAAGTAAATGATAAAGATGAACTA
    AAAAATCAATCCAGATTAATTGCTGATTCAGAAGAAGATGGAGCAACGAATAAAGAAGAA
    TATTCAGGAAGTCAAATCGATGATGCAGAATTTTATGAATTAAATGATACAGAAGTAGAT
    GAGGATACTACTTCAAATATCGAAGATAATACCAATAGAAACGCGTCTGAAATGCATGTA
    GACGCTCCTAAAACGCAAGAGTACGCAGTAACTGAATCTCAAGTAAATAATATCGATAAA
    ACGGTTGATAATGAAATTGAATTAGCACCGCGTCATAAAAAAGATGACCAAACAAACTTA
    AGTGTCAACTCATTGAAAACGAATGATGTGAATGATAATCATGTTGTGGAAGATTCAAGC
    ATGAATGAAATAGAAAAGAATAACGCAGAAATTACAGAAAATGTGCAAAACGAAGCAGCT
    GAAAGTGAACAAAATGTCGAAGAGAAAACTATTGAAAACGTAAATCCAAAGAAACAGACT
    GAAAAGGTTTCAACTTTAAGTAAAAGACCATTTAATGTTGTCATGACGCCATCTGATAAA
    AAGCGTATGATGGATCGTAAAAAGCATTCAAAAGTCAATGTGCCTGAATTAAAGCCTGTA
    CAAAGTAAGCAAGCTGTGAGTGAAAGAATGCCTGCGAGTCAAGCCACACCATCATCAAGA
    TCTGATTCACAAGAGTCAAATACAAATGCATATAAAACAAATAATATGACATCAAACAAT
    GTTGAGAACAATCAACTTATTGGTCATGCAGAAACAGAAAATGATTATCAAAATGCACAA
    CAATATTCAGAGCAGAAACCTTCTGTTGATTCAACTCAAACGGAAATATTTGAAGAAAGT
    CAAGATGATAATCAATTGGAAAATGAGCAAGTTGATCAATCAACTTCGTCTTCAGTTTCA
    GAAGTAAGCGACATAACTGAAGAAAGCGAAGAAACAACACATCCAAACAATACTAGTGGA
    CAACAAGATAATGATGATCAACAAAAAGATTTACAGTCATCATTTTCAAATAAAAATGAA
    GATACAGCTAATGAAAATAGACCTCGGACGAACCAACAAGATGTTGCAACAAATCAAGCT
    GTACAAACATCTAAGCCGATGATTCGTAAAGGCCCAAATATTAAATTGCCAAGTGTTTCA
    TTACTAGAAGAACCACAAGTTATTGAGTCGGACGAGGACTGGATTACAGATAAAAAGAAA
    GAACTGAATGACGCATTATTTTACTTTAATGTACCTGCAGAAGTACAAGATGTAACTGAA
    GGTCCAAGTGTTACAAGATTTGAATTATCAGTTGAAAAAGGTGTTAAAGTTTCAAGAATT
    ACGGCATTACAAGATGACATTAAAATGGCATTGGCAGCGAAAGATATTCGTATAGAAGCG
    CCTATTCCAGGAACTAGTCGTGTTGGTATTGAAGTTCCGAACCAAAATCCAACGACAGTC
    AACTTACGTTCTATTATTGAATCTCCAAGTTTTAAAAATGCTGAATCTAAATTAACAGTT
    GCGATGGGGTATAGAATTAATAATGAACCATTACTTATGGATATTGCTAAAACGCCACAC
    GCACTAATTGCAGGTGCAACTGGATCAGGGAAATCAGTTTGTATCAATAGTATTTTGATG
    TCTTTACTATATAAAAATCATCCTGAGGAATTAAGATTATTACTTATCGATCCAAAAATG
    GTTGAATTAGCTCCTTATAATGGTTTGCCACATTTAGTTGCACCGGTAATTACAGATGTC
    AAAGCAGCTACACAGAGTTTAAAATGGGCCGTAGAAGAAATGGAACGACGTTATAAGTTA
    TTTGCACATTACCATGTACGTAATATAACAGCATTTAACAAAAAAGCACCATATGATGAA
    AGAATGCCAAAAATTGTCATTGTAATTGATGAGTTGGCTGATTTAATGATGATGGCTCCG
    CAAGAAGTTGAACAGTCTATTGCTAGAATTGCTCAAAAAGCGAGAGCATGTGGTATTCAT
    ATGTTAGTAGCTACGCAAAGACCATCTGTCAATGTAATTACAGGTTTAATTAAAGCCAAC
    ATACCAACAAGAATTGCATTTATGGTATCATCAAGTGTAGATTCGAGAACGATATTAGAC
    AGTGGTGGAGCAGAACGCTTGTTAGGATATGGCGATATGTTATATCTTGGTAGCGGTATG
    AATAAACCGATTAGAGTTCAAGGTACATTTGTTTCTGATGACGAAATTGATGATGTTGTT
    GATTTTATCAAACAACAAAGAGAACCGGACTATCTATTTGAAGAAAAAGAATTGTTGAAA
    AAAACACAAACACAATCACAAGATGAATTATTTGATGATGTTTGTGCATTTATGGTTAAT
    GAAGGACATATTTCAACATCATTAATCCAAAGACATTTCCAAATTGGCTATAATAGAGCA
    GCAAGAATTATCGATCAATTAGAGCAACTCGGTTATGTTTCGAGTGCTAATGGTTCAAAA
    CCAAGGGATGTTTATGTTACGGAAGCAGATTTAAATAAAGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2460
    2520
    2580
    2640
    2700
    2760
    2820
    2880
    2940
    3000
    3060
    3120
    3180
    3240
    3300
    3360
    3420
    3480
    3540
    3600
    3660
    3720
    3780
    3825

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_01857
  • symbol: SAOUHSC_01857
  • description: hypothetical protein
  • length: 1274
  • theoretical pI: 4.84013
  • theoretical MW: 144655
  • GRAVY: -1.01593

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EssC (TIGR03928; HMM-score: 129.6)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EccCa (TIGR03924; HMM-score: 107.4)
    and 1 more
    Genetic information processing Protein fate Protein and peptide secretion and trafficking type VII secretion protein EccCb (TIGR03925; HMM-score: 87.1)
  • TheSEED  :
    • Cell division protein FtsK
    Cell Division and Cell Cycle Cell Division and Cell Cycle - no subcategory Bacterial Cytoskeleton  Cell division protein FtsK
  • PFAM:
    P-loop_NTPase (CL0023) FtsK_SpoIIIE; FtsK/SpoIIIE family (PF01580; HMM-score: 194.1)
    and 3 more
    HTH (CL0123) Ftsk_gamma; Ftsk gamma domain (PF09397; HMM-score: 95.9)
    P-loop_NTPase (CL0023) AAA_22; AAA domain (PF13401; HMM-score: 18.5)
    AAA_29; P-loop containing region of AAA domain (PF13555; HMM-score: 14)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 1.05
    • Cytoplasmic Membrane Score: 8.78
    • Cellwall Score: 0.08
    • Extracellular Score: 0.09
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005444
    • TAT(Tat/SPI): 0.001518
    • LIPO(Sec/SPII): 0.001815
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNNDIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRRNQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKPKTHNMYSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQKYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIKIENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQAERYVGDSSLNDDSDLTDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDESPYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADSEEDGATNKEEYSGSQIDDAEFYELNDTEVDEDTTSNIEDNTNRNASEMHVDAPKTQEYAVTESQVNNIDKTVDNEIELAPRHKKDDQTNLSVNSLKTNDVNDNHVVEDSSMNEIEKNNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMMDRKKHSKVNVPELKPVQSKQAVSERMPASQATPSSRSDSQESNTNAYKTNNMTSNNVENNQLIGHAETENDYQNAQQYSEQKPSVDSTQTEIFEESQDDNQLENEQVDQSTSSSVSEVSDITEESEETTHPNNTSGQQDNDDQQKDLQSSFSNKNEDTANENRPRTNQQDVATNQAVQTSKPMIRKGPNIKLPSVSLLEEPQVIESDEDWITDKKKELNDALFYFNVPAEVQDVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPNQNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKSVCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWAVEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIARIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAERLLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQTQSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPRDVYVTEADLNKE

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. 3.0 3.1 3.2 3.3 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]