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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01875
  • pan locus tag?: SAUPAN004441000
  • symbol: leuS
  • pan gene symbol?: leuS
  • synonym:
  • product: leucyl-tRNA synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01875
  • symbol: leuS
  • product: leucyl-tRNA synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1788818..1791235
  • length: 2418
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    GTGTTGAATTACAACCACAATCAAATTGAAAAGAAATGGCAAGACTATTGGGACGAAAAT
    AAAACATTTAAAACAAATGATAACTTAGGTCAAAAGAAATTTTATGCTTTAGACATGTTT
    CCATATCCATCAGGTGCTGGTTTACATGTTGGACATCCTGAGGGCTATACAGCAACAGAT
    ATCATTTCAAGATATAAAAGAATGCAAGGATATAATGTATTACATCCGATGGGGTGGGAT
    GCATTCGGATTACCAGCAGAGCAATATGCTTTAGACACTGGCAACGACCCACGTGAATTT
    ACAAAGAAAAATATCCAAACTTTTAAACGACAAATTAAAGAATTAGGGTTCAGTTATGAT
    TGGGATCGTGAAGTTAATACAACAGATCCAGAATACTATAAATGGACACAGTGGATTTTC
    ATACAGTTATATAACAAAGGTTTAGCATACGTTGATGAAGTTGCAGTTAACTGGTGTCCA
    GCATTAGGCACTGTTTTATCTAACGAAGAAGTGATTGATGGTGTCTCTGAACGTGGTGGA
    CATCCAGTTTATCGTAAGCCGATGAAACAATGGGTACTTAAAATCACAGAATATGCAGAT
    CAATTATTAGCAGATTTAGATGATTTAGATTGGCCTGAGTCTTTAAAAGATATGCAGCGC
    AATTGGATTGGACGTTCTGAAGGGGCCAAAGTTTCATTTGATGTAGATAATACGGAAGGA
    AAAGTAGAAGTATTTACGACTAGACCAGATACAATCTATGGTGCATCATTCTTAGTCTTA
    AGTCCTGAACATGCATTAGTTAATTCAATTACAACAGATGAATATAAAGAAAAAGTAAAA
    GCTTATCAAACAGAAGCTTCTAAAAAGTCAGATTTAGAACGTACAGATTTAGCAAAAGAT
    AAATCAGGTGTATTTACTGGTGCATATGCAACTAATCCTTTATCTGGTGAAAAAGTACAA
    ATTTGGATTGCTGATTATGTATTATCAACATATGGTACTGGAGCAATTATGGCAGTACCA
    GCGCATGATGACAGAGATTATGAATTTGCTAAAAAGTTTGATTTGCCAATCATTGAAGTC
    ATCGAAGGTGGAAATGTTGAAGAAGCAGCATACACTGGTGAAGGTAAACATATTAATTCT
    GGTGAACTTGATGGTTTAGAAAATGAAGCGGCAATTACTAAAGCTATTCAATTATTAGAG
    CAAAAAGGTGCTGGCGAAAAGAAAGTTAATTACAAATTAAGAGATTGGTTATTCAGTCGT
    CAGCGTTATTGGGGCGAACCAATTCCTGTCATTCATTGGGAAGATGGAACAATGACAACT
    GTTCCTGAAGAAGAGCTACCATTGTTGTTACCTGAAACAGATGAAATCAAGCCATCAGGG
    ACTGGTGAGTCTCCACTAGCTAATATTGATTCATTTGTAAATGTTGTAGATGAAAAAACA
    GGTATGAAAGGACGTCGTGAAACAAATACAATGCCACAATGGGCAGGTAGTTGTTGGTAT
    TATTTACGTTACATCGATCCTAAAAATGAAAATATGTTAGCAGATCCTGAAAAATTAAAA
    CATTGGTTACCTGTTGATTTATATATCGGTGGAGTAGAACATGCGGTTCTTCACTTATTA
    TATGCAAGATTTTGGCATAAAGTCCTTTATGATTTGGCTATCGTACCTACTAAAGAACCT
    TTCCAAAAATTATTTAACCAAGGTATGATTTTAGGAGAAGGTAATGAGAAGATGAGTAAA
    TCTAAAGGAAATGTAATCAATCCTGATGATATAGTACAGTCTCATGGTGCAGATACTTTG
    CGTCTTTACGAAATGTTTATGGGACCTTTAGATGCTGCAATTGCATGGAGTGAAAAAGGA
    TTAGATGGGTCTCGTCGATTCTTAGATCGCGTATGGCGTTTAATGGTAAATGAAGATGGG
    ACATTGAGTTCAAAAATTGTAACTACAAATAATAAATCTTTAGATAAAGTTTATAACCAA
    ACTGTTAAAAAGGTAACAGAAGACTTTGAAACATTAGGATTTAATACTGCTATTAGTCAA
    TTAATGGTATTTATTAATGAGTGTTATAAAGTTGATGAAGTTTATAAACCTTACATTGAA
    GGCTTCGTTAAAATGTTAGCACCTATTGCACCACATATCGGTGAAGAATTATGGTCAAAA
    TTAGGACATGAAGAGTCTATTACGTACCAACCTTGGCCAACTTATGACGAAGCACTACTT
    GTAGATGATGAAGTAGAAATCGTTGTTCAAGTGAATGGTAAATTGAGAGCTAAAATTAAA
    ATTGCTAAAGATACATCAAAAGAAGAAATGCAAGAAATTGCCTTATCTAATGACAATGTT
    AAAGCGAGTATTGAAGGTAAAGACATCATGAAAGTCATCGCTGTTCCTCAAAAATTAGTC
    AATATTGTAGCTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2418

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01875
  • symbol: LeuS
  • description: leucyl-tRNA synthetase
  • length: 805
  • theoretical pI: 4.78881
  • theoretical MW: 91785.3
  • GRAVY: -0.498509

Function[edit source | edit]

  • reaction:
    EC 6.1.1.4?  ExPASy
    Leucine--tRNA ligaseATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 1186.6)
    Genetic information processingProtein synthesistRNA aminoacylationvaline--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 310.1)
    Genetic information processingProtein synthesistRNA aminoacylationisoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 228.8)
    Genetic information processingProtein synthesistRNA aminoacylationleucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 227.8)
    Genetic information processingProtein synthesistRNA aminoacylationmethionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 114.3)
    Genetic information processingProtein synthesistRNA aminoacylationcysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 37.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersGlutathione and analogscysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 26.2)
  • TheSEED:  
    Leucyl-tRNA synthetase (EC 6.1.1.4) 
  • PFAM:
    HUP (CL0039) tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 245.4)
    tRNA-synt_1_2; Leucyl-tRNA synthetase, Domain 2 (PF13603; HMM-score: 227)
    tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 96.9)
    DALR (CL0258) Anticodon_1; Anticodon-binding domain of tRNA (PF08264; HMM-score: 82.7)
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 25.9)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 18.3)
    tRNA-synt_1c; tRNA synthetases class I (E and Q), catalytic domain (PF00749; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.117
    • Ymax_pos: 18
    • Cmax: 0.103
    • Cmax_pos: 13
    • Smax: 0.192
    • Smax_pos: 7
    • Smean: 0.122
    • D: 0.119
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPSGAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTKKNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCPALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDMQRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYKEKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYATNPLSGEKVQIWIADYVLSTYGTGAIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAITKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLLPETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPKNENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLAIVPTKEPFQKLFNQGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGSRRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLMVFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTYDEALLVDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQKLVNIVAK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 2.35 2.36 2.37 2.38 2.39 2.40 2.41 2.42 2.43 2.44 2.45 2.46 2.47 2.48 2.49 2.50 2.51 2.52 2.53 2.54 2.55 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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