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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01877
  • pan locus tag?: SAUPAN004446000
  • symbol: SAOUHSC_01877
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01877
  • symbol: SAOUHSC_01877
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1792814..1793767
  • length: 954
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGGCAATCATTTCCAATACGCTTTTGAAAACAAACGTTATCACACATGGAATTACCAT
    TTAAAAAATAAATTTGGACAAAAAATATTTAAAGTTGCATTGGATGGCGGGTTTGACTGT
    CCTAACCGCGATGGCACTGTAGCACATGGTGGATGTACATTTTGTTCTGCTGCAGGTAGC
    GGAGACTTTGCAGGTAATCGTGCAGATTCAATCGCAGTACAATTTAAAGAAATTAAAGAA
    AAGATGCATGAGAAATGGCACGAAGGAAAATATATTGCTTATTTTCAGGCATTTACAAAT
    ACACATGCACCGGTTGAAGTATTAAAAGAAAAATTCGAACCTGTACTTAAAGAACCGGGT
    GTTGTGGGATTATCTATTGGTACGCGTCCTGACTGTCTACCAGACGATGTTGTCGAATAT
    TTAGCAGATTTGAATCAACGAACATACTTATGGGTTGAATTAGGACTACAAACAATCCAT
    CAGTCAACATCTGATTTAATCAATCGTGCCCATGATATGAAAACTTATTATGATGGTGTG
    GCAAAATTACGTAAGCATAATATCAATGTATGTACACACATCATTAATGGCTTACCTGGC
    GAAGACTATGACATGATGATGGCTACTGCCAAAGAAGTTGCACAAATGGATGTACAAGGT
    ATTAAAATTCATTTACTTCATTTGTTAAAAGGTACACCGATGGTAAAACAATACGATAAA
    GGTTTATTAACTTTTATGACTCAAGAAGAGTACACAAACCTAGTTGTGGACCAATTAGAA
    GTGATTCCCCCTGAAATGATCGTTCACCGAATTACCGGTGATGGTCCAATAGATATCATG
    GTAGGTCCAATGTGGAGTGTTAATAAATGGGAAGTATTAAATGGCATCGATGCTGAATTA
    GCACGTAGAAATTCTTATCAAGGCTTGCGTTACAAGTCTAAGGTGAAGCAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    954

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01877
  • symbol: SAOUHSC_01877
  • description: hypothetical protein
  • length: 317
  • theoretical pI: 7.03345
  • theoretical MW: 36053.1
  • GRAVY: -0.393691

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityradical SAM protein, TIGR01212 family (TIGR01212; HMM-score: 433.5)
    Genetic information processingTranscriptionDNA-dependent RNA polymeraseradical SAM enzyme/protein acetyltransferase, ELP3 family (TIGR01211; EC 2.3.1.48; HMM-score: 63)
    Unknown functionEnzymes of unknown specificityB12-binding domain/radical SAM domain protein, MJ_1487 family (TIGR04013; HMM-score: 39.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalamincoproporphyrinogen dehydrogenase HemZ (TIGR03994; EC 1.3.99.22; HMM-score: 38.8)
    putative heme utilization radical SAM enzyme HutW (TIGR04107; HMM-score: 32)
    radical SAM methylthiotransferase, MiaB/RimO family (TIGR00089; EC 2.1.1.-,2.8.1.-; HMM-score: 28.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinbiotin synthase (TIGR00433; EC 2.8.1.6; HMM-score: 27.3)
    Hypothetical proteinsConservedradical SAM enzyme, TIGR01210 family (TIGR01210; HMM-score: 24.4)
    Unknown functionEnzymes of unknown specificityB12-binding domain/radical SAM domain protein, MJ_0865 family (TIGR04014; HMM-score: 22.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminoxygen-independent coproporphyrinogen III oxidase (TIGR00538; EC 1.3.99.22; HMM-score: 22.8)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA-i(6)A37 thiotransferase enzyme MiaB (TIGR01574; EC 2.-.-.-; HMM-score: 19.6)
    Cellular processesCellular processesToxin production and resistanceradical SAM P-methyltransferase, PhpK family (TIGR04479; EC 2.1.-.-; HMM-score: 18.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationMiaB-like tRNA modifying enzyme (TIGR01579; HMM-score: 17.3)
    Genetic information processingProtein synthesistRNA and rRNA base modificationMiaB-like tRNA modifying enzyme, archaeal-type (TIGR01578; HMM-score: 15.1)
    hopanoid biosynthesis associated radical SAM protein HpnJ (TIGR03471; HMM-score: 14.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminputative oxygen-independent coproporphyrinogen III oxidase (TIGR00539; EC 1.3.99.22; HMM-score: 12.3)
  • TheSEED:  
    Uncharacterized radical SAM protein YtqA 
  • PFAM:
    no clan definedRadical_SAM_C; Radical_SAM C-terminal domain (PF16199; HMM-score: 82.9)
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 52.9)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.126
    • Ymax_pos: 38
    • Cmax: 0.104
    • Cmax_pos: 65
    • Smax: 0.21
    • Smax_pos: 25
    • Smean: 0.141
    • D: 0.132
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRDGTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTHAPVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIHQSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDVQGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGPIDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]