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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01878
  • pan locus tag?: SAUPAN004447000
  • symbol: SAOUHSC_01878
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01878
  • symbol: SAOUHSC_01878
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1793764..1794327
  • length: 564
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAAATTAGAACGTATACTCCCTTTTTCAAAAACACTTATTAAACAACATATAACACCA
    GAAAGTATTGTTGTAGACGCAACTTGCGGTAACGGCAATGACACTTTATTTTTAGCCGAA
    CAAGTACCAGAAGGACATGTTTATGGTTTCGACATTCAAGATTTAGCTTTGGAAAATACA
    CGTGATAAAGTTAAGGATTTCAATCATGTTTCTTTAATAAAAGATGGACATGAAAATATT
    GAACATCATATAAATGATGCACATAAAGGTCATATTGATGCAGCCATCTTTAACCTAGGT
    TATTTGCCTAAAGGTGATAAATCTATCGTGACAAAGCCTGACACGACAATCCAAGCTATT
    AATTCATTGCTATCATTAATGTCAATTGAAGGTATTATTGTACTTGTTATATATCATGGT
    CATAGCGAAGGACAAATTGAGAAGCATGCATTGCTTGATTACTTGAGTACTTTGGATCAA
    AAGCATGCGCAAGTTTTGCAATATCAATTTTTAAACCAACGTAATCATGCTCCATTCATT
    TGTGCCATAGAAAAAATTTCTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    564

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01878
  • symbol: SAOUHSC_01878
  • description: hypothetical protein
  • length: 187
  • theoretical pI: 6.31001
  • theoretical MW: 20992.8
  • GRAVY: -0.181283

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modification16S rRNA (cytosine(1402)-N(4))-methyltransferase (TIGR00006; EC 2.1.1.199; HMM-score: 31)
    Genetic information processingProtein fateProtein modification and repairprotein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 21.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprecorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 20.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinonedemethylmenaquinone methyltransferase (TIGR02752; EC 2.1.1.163; HMM-score: 20.5)
    Genetic information processingProtein synthesisRibosomal proteins: synthesis and modificationputative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 20.5)
    Genetic information processingProtein synthesistRNA and rRNA base modification23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 19.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinmalonyl-acyl carrier protein O-methyltransferase BioC (TIGR02072; EC 2.1.1.-; HMM-score: 15.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 15)
    2-ketoarginine methyltransferase (TIGR04543; EC 2.1.1.243; HMM-score: 14.8)
    Unknown functionEnzymes of unknown specificitytRNA (cmo5U34)-methyltransferase (TIGR00740; EC 2.1.1.-; HMM-score: 14.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllmagnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 11.4)
  • TheSEED:  
    Putative rRNA methylase YtqB 
  • PFAM:
    NADP_Rossmann (CL0063) rRNA_methylase; Putative rRNA methylase (PF06962; HMM-score: 164.5)
    Methyltransf_5; MraW methylase family (PF01795; HMM-score: 35.9)
    Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 35.1)
    Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 33.7)
    MTS; Methyltransferase small domain (PF05175; HMM-score: 20.2)
    Methyltransf_12; Methyltransferase domain (PF08242; HMM-score: 19.4)
    Methyltransf_32; Methyltransferase domain (PF13679; HMM-score: 18.9)
    Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 15.8)
    TPMT; Thiopurine S-methyltransferase (TPMT) (PF05724; HMM-score: 15)
    Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 13.8)
    Methyltransf_18; Methyltransferase domain (PF12847; HMM-score: 13.3)
    Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 12.9)
    GCD14; tRNA methyltransferase complex GCD14 subunit (PF08704; HMM-score: 12.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.185
    • Ymax_pos: 23
    • Cmax: 0.116
    • Cmax_pos: 39
    • Smax: 0.381
    • Smax_pos: 21
    • Smean: 0.296
    • D: 0.228
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPEGHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDAHKGHIDAAIFNLGYLPKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQKHAQVLQYQFLNQRNHAPFICAIEKIS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]