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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01909
  • pan locus tag?: SAUPAN004501000
  • symbol: SAOUHSC_01909
  • pan gene symbol?: metK
  • synonym:
  • product: S-adenosylmethionine synthetase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_01909
  • symbol: SAOUHSC_01909
  • product: S-adenosylmethionine synthetase
  • replicon: chromosome
  • strand: -
  • coordinates: 1817570..1818763
  • length: 1194
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGTTAAATAACAAACGATTATTTACTTCAGAGTCTGTTACAGAAGGACACCCAGATAAA
    ATCGCTGACCAAGTGTCAGATGCAATATTAGATGCTATTTTAAAAGACGATCCAAATGCA
    CGTGTAGCTTGTGAAACAACTGTTACAACAGGTATGGCATTGATCGCCGGCGAAATTTCT
    ACAACAACATATGTTGATATTCCGAAAGTTGTTAGAGAAACAATTAAAGAAATTGGATAC
    ACAAGAGCAAAATATGGTTATGATTATGAAACAATGGCAATTTTAACTGCGATTGATGAA
    CAATCACCTGACATTGCACAAGGCGTGGATAAAGCATTAGAGTATCGTGATAAAGATAGC
    GAAGAAGAAATTGAAGCAACAGGTGCAGGTGACCAAGGTTTAATGTTTGGTTACGCAACG
    AATGAAACAGAGACGTATATGCCTCTAGCAATTTATTTATCACATCAATTGGCAAAACGT
    TTATCAGATGTGCGTAAAGATGGTACGTTAAATTATTTACGACCAGACGGTAAAGTTCAG
    GTCACTGTTGAATATGATGAAAATGATAACCCAGTACGTATTGATACGATTGTCGTATCA
    ACACAACATGCTGAGGATGTGACACTTGAACAAATTCAAGAAGACATTAAAGCACATGTC
    ATTTATCCAACTGTTCCAGAAAACTTGATTAATGAACAAACTAAATTCTACATCAATCCT
    ACAGGACGCTTTGTAATCGGTGGACCTCAAGGTGATGCAGGATTAACAGGACGTAAAATC
    ATTGTTGATACGTACGGTGGCTATGCACGTCATGGTGGCGGATGCTTCAGTGGTAAGGAT
    CCTACAAAAGTAGACCGTTCAGCTGCATACGCTGCACGTTATGTGGCTAAAAATATTGTT
    GCAGCAGGTTTAGCAGATCAATGTGAAGTACAATTGGCATATGCGATTGGTGTTGCAGAA
    CCAGTGTCAATTGCAATTGATACATTTGGAACAGGTAAAGTTTCTGAAGGGCAACTTGTC
    GAAGCAGTTAGAAAACACTTTGACCTAAGACCTGCAGGTATTATTAAAATGCTTGATTTG
    AAACAACCGATTTACAAACAAACTGCTGCTTATGGTCATTTTGGACGTACAGATGTCTTA
    TTCCCATGGGAAAAATTAGACAAAGTGGAAGAATTAAAAGACGCAGTAAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1194

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_01909
  • symbol: SAOUHSC_01909
  • description: S-adenosylmethionine synthetase
  • length: 397
  • theoretical pI: 4.62015
  • theoretical MW: 43640.9
  • GRAVY: -0.325441

Function[edit source | edit]

  • reaction:
    EC 2.5.1.6?  ExPASy
    Methionine adenosyltransferaseATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine
  • TIGRFAM:
    MetabolismCentral intermediary metabolismOthermethionine adenosyltransferase (TIGR01034; EC 2.5.1.6; HMM-score: 621.4)
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineMethionine Biosynthesis S-adenosylmethionine synthetase (EC 2.5.1.6) 
    Lysine, threonine, methionine, and cysteineMethionine Degradation S-adenosylmethionine synthetase (EC 2.5.1.6) 
    Cofactors, Vitamins, Prosthetic Groups, PigmentsFolate and pterinesFolate Biosynthesis S-adenosylmethionine synthetase (EC 2.5.1.6) 
  • PFAM:
    no clan definedS-AdoMet_synt_C; S-adenosylmethionine synthetase, C-terminal domain (PF02773; HMM-score: 238.2)
    S-AdoMet_synt_M; S-adenosylmethionine synthetase, central domain (PF02772; HMM-score: 162.9)
    S-AdoMet_synt_N; S-adenosylmethionine synthetase, N-terminal domain (PF00438; HMM-score: 145.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Co2+, K+, Mg2+
  • effectors:
  • protein partners:
    SAOUHSC_02494(rpsE)30S ribosomal protein S5  [2] (data from MRSA252)
    SAOUHSC_01819hypothetical protein  [2] (data from MRSA252)

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.155
    • Ymax_pos: 11
    • Cmax: 0.14
    • Cmax_pos: 23
    • Smax: 0.352
    • Smax_pos: 1
    • Smean: 0.211
    • D: 0.177
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVACETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQSPDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKRLSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKAHVIYPTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCFSGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKVSEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEELKDAVK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. 2.0 2.1 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]