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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02090
  • pan locus tag?: SAUPAN004862000
  • symbol: SAOUHSC_02090
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02090
  • symbol: SAOUHSC_02090
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1966974..1968119
  • length: 1146
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGCGTAATAACAAGCCTAAAATAAGTATTCAAAACGACCCATGGGAAGCATATAACGAT
    GTTAAAGAACATGGCCAATTAACTTTAAGTAACATCGAATTTACAACTACAAATCTTTGT
    AATATGCGTTGTAGCCACTGTGCAGTTGGTTATACTTTACAAACTGTCGACCCCGAGCCT
    TTAGATATGGACTTAATTTATCGTAGACTTGATGAAATTCCAAATCTGCGAACGATGTCT
    ATTACAGGTGGCGAACCAATGTTTTCTAAAAAGTCTATTAGAAATGTTGTTAAACCTCTA
    TTAAAGTATGCACATCATCGAGGTATATATACACAAATGAATTCAAACCTAACATTGCCT
    CAAGATCGTTATTTAGATATTGCTGAATATATCGATGTTATGCATATCTCACACAACTGG
    GGAACAACTGATGAATTCGCAAATGTTGGCTTTGGCGCAATGAAGAAGCAACCACCGTTA
    AAAGCTAAGTTAAAATTATATGAACAAATGATTTCGAATGCACGTACATTATCAGAACAA
    GGAATGTTTGTATCTGCGGAAACAATGCTCAATCAAAGTACGCTACCACATTTACGAAAA
    ATACATCAAGAAGTCGTTCATGATATGAAATGTAGCAGACACGAGATTCACCCTATGTAT
    CCAGCTGACTTTGCAAGTCAATTAAATGTGTTAACTCTAGCGGAAATGAAAAAGACAATT
    CATGATATATTGGATTTCAGAGATGAAGATATTTGGATGTTATTTGGTACTTTGCCTGTG
    TTTCCATGCTTAAAGGATGATGAAGATCAAAAGTTACTATCACGTTTAAGAAATGCTAAA
    AATGTAACGACTAGAAATGACCCGGATGGCCGTAGTCGTTTAAATGTCAATGTATTTACA
    GGTAATGTAATCGTAACTGATTTCGGAGATGAAACAGGTACAATTTCGAATATACAAAAA
    GATAAATTAACAGATGTATTTGATAAATGGTTATCCTCTGATCTTGCTAAATCATTAAAT
    TGTCATTGTTCCGAGTTTAGTTGTTTAGGGCCAAATGTTCTTGTTAAAAATATGTACTAT
    CCGAATATGGATTTTAAAGATAATGAGCGTCATATGCACAAACAACCACAAATTATACAA
    TTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1146

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02090
  • symbol: SAOUHSC_02090
  • description: hypothetical protein
  • length: 381
  • theoretical pI: 6.91373
  • theoretical MW: 43987.1
  • GRAVY: -0.516535

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityradical SAM/CxCxxxxC motif protein YfkAB (TIGR04478; HMM-score: 616.2)
    Genetic information processingProtein synthesistRNA and rRNA base modificationputative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR03963; HMM-score: 27.6)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminputative heme d1 biosynthesis radical SAM protein NirJ2 (TIGR04055; EC 1.3.99.-; HMM-score: 27.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminputative heme d1 biosynthesis radical SAM protein NirJ1 (TIGR04054; HMM-score: 24.7)
    radical SAM/Cys-rich domain protein (TIGR04167; HMM-score: 24.6)
    tungsten cofactor oxidoreducase radical SAM maturase (TIGR04317; HMM-score: 24.6)
    Cellular processesCellular processesToxin production and resistanceCys-rich peptide radical SAM maturase CcpM (TIGR04068; HMM-score: 24.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMolybdopterinmolybdenum cofactor biosynthesis protein A (TIGR02666; HMM-score: 23.4)
    Unknown functionEnzymes of unknown specificityradical SAM protein, BA_1875 family (TIGR04053; HMM-score: 23.4)
    hopanoid biosynthesis associated radical SAM protein HpnH (TIGR03470; HMM-score: 23.3)
    His-Xaa-Ser system radical SAM maturase HxsC (TIGR03977; HMM-score: 22.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminheme b synthase (TIGR04545; EC 1.3.99.-; HMM-score: 21.8)
    pseudo-rSAM protein/SPASM domain protein (TIGR04347; HMM-score: 20.1)
    Genetic information processingProtein synthesistRNA and rRNA base modification7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR03365; HMM-score: 19.6)
    His-Xaa-Ser system radical SAM maturase HxsB (TIGR03978; HMM-score: 19.2)
    SynChlorMet cassette radical SAM/SPASM protein ScmF (TIGR04251; HMM-score: 18.8)
    Unknown functionEnzymes of unknown specificitymycofactocin radical SAM maturase (TIGR03962; HMM-score: 18.6)
    putative 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE (TIGR04349; HMM-score: 18)
    Genetic information processingProtein fateProtein modification and repairanaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02491; EC 1.97.1.-; HMM-score: 17.2)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismanaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02491; EC 1.97.1.-; HMM-score: 17.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminheme d1 biosynthesis radical SAM protein NirJ (TIGR04051; HMM-score: 17.2)
    radical SAM/SPASM domain protein maturase (TIGR04463; HMM-score: 17.1)
    Genetic information processingProtein fateProtein modification and repairpyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 16.7)
    MetabolismEnergy metabolismAnaerobicpyruvate formate-lyase 1-activating enzyme (TIGR02493; EC 1.97.1.4; HMM-score: 16.7)
    radical SAM protein, TatD family-associated (TIGR04038; HMM-score: 16.7)
    Genetic information processingProtein fateProtein modification and repairanaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 16.6)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismanaerobic ribonucleoside-triphosphate reductase activating protein (TIGR02495; EC 1.97.-.-; HMM-score: 16.6)
    Unknown functionEnzymes of unknown specificityarchaeal radical SAM protein, PTO1314 family (TIGR03961; HMM-score: 16.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMolybdopterinprobable molybdenum cofactor biosynthesis protein A (TIGR02668; HMM-score: 16.2)
    SynChlorMet cassette radical SAM/SPASM protein ScmE (TIGR04250; HMM-score: 16)
    Signal transductionRegulatory functionsProtein interactionspeptide modification radical SAM enzyme, YydG family (TIGR04078; HMM-score: 15.7)
    putative geopeptide radical SAM maturase (TIGR04280; HMM-score: 15.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalamin12,18-didecarboxysiroheme deacetylase (TIGR04546; HMM-score: 15.5)
    radical SAM peptide maturase, GG-Bacteroidales family (TIGR04148; HMM-score: 14.9)
    glycyl-radical enzyme activating protein (TIGR02494; EC 1.97.1.-; HMM-score: 14.4)
    GeoRSP system radical SAM/SPASM protein (TIGR04303; HMM-score: 14.3)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOther7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase, CofH subunit (TIGR03551; EC 2.5.1.77; HMM-score: 13.2)
    Cellular processesCellular processesAdaptations to atypical conditionsglutamate 2,3-aminomutase (TIGR04368; EC 5.4.3.9; HMM-score: 13.2)
    putative peptide-modifying radical SAM enzyme, AF0577 family (TIGR04084; HMM-score: 12.9)
  • TheSEED:  
    CBSS-176280.1.peg.1561 Hypothetical protein SAV1877 
  • PFAM:
    no clan definedYfkB; YfkB-like domain (PF08756; HMM-score: 250.8)
    TIM_barrel (CL0036) Radical_SAM; Radical SAM superfamily (PF04055; HMM-score: 44.4)
    4Fe-4S (CL0344) Fer4_14; 4Fe-4S single cluster domain (PF13394; HMM-score: 24.5)
    Fer4_12; 4Fe-4S single cluster domain (PF13353; HMM-score: 23.8)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.157
    • Ymax_pos: 48
    • Cmax: 0.165
    • Cmax_pos: 48
    • Smax: 0.245
    • Smax_pos: 45
    • Smean: 0.106
    • D: 0.137
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MRNNKPKISIQNDPWEAYNDVKEHGQLTLSNIEFTTTNLCNMRCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPLLKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQPPLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHDMKCSRHEIHPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLSRLRNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLSSDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

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