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PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02193
  • pan locus tag?: SAUPAN001538000
  • symbol: SAOUHSC_02193
  • pan gene symbol?:
  • synonym:
  • product: prohead protease

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02193
  • symbol: SAOUHSC_02193
  • product: prohead protease
  • replicon: chromosome
  • strand: -
  • coordinates: 2053338..2053922
  • length: 585
  • essential: no DEG

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGAGTAAGGAAACGAGAGTTGGCAACATTATTGAGGTACGCTCAAATGATAACAACGAA
    ATGGTCATAGAGGGGTATGCGTTAAAGTTTGACACTTGGTCTGAAAATCTTGGTGGATTC
    AAAGAAACGATTTCACGTCGCGCTTTAGAAAACACTGATTTATCTGATGTGCGTTGTTTA
    GTAGATCATATCCCATCGCAAATAATTGGTAGGACAAAATCGGGTACTTTGGAGCTCGAA
    ACTGATGATGTTGGACTTAAATATCGTTGTAAGTTACCAAACACAACATTTGCACGTGAT
    TTATATGAGAACATGCGTGTAGGCAACATCAATCAATGTTCGTTTGGTTTTATGCTTGAC
    GATAAAGGCGATGAAGTGCGTTTTGATGAACAAGAAAACATTTACAAACGTACTTTAACA
    GCAATTCGTGAACTTACAGATGTTTCTGTAGTGACTTATCCGGCTTACAAAGACACTGAT
    GTTAAACCAGCATTACGTAGTATTGAAACCGTTAAAAAAGAACAACGTAAAAAAGAATTA
    GAAATAAGACTAAAGAAACACTCTATATTAAATAATATTTGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    585

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_02193
  • symbol: SAOUHSC_02193
  • description: prohead protease
  • length: 194
  • theoretical pI: 5.91304
  • theoretical MW: 22457.3
  • GRAVY: -0.647938

Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing Mobile and extrachromosomal element functions Prophage functions phage prohead protease, HK97 family (TIGR01543; EC 3.4.-.-; HMM-score: 95.5)
    Genetic information processing Protein fate Other phage prohead protease, HK97 family (TIGR01543; EC 3.4.-.-; HMM-score: 95.5)
  • TheSEED  :
    • Phage head maturation protease
  • PFAM:
    Peptidase_SH (CL0201) Peptidase_S78; Caudovirus prohead serine protease (PF04586; HMM-score: 195.9)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.003964
    • TAT(Tat/SPI): 0.000292
    • LIPO(Sec/SPII): 0.000362
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MSKETRVGNIIEVRSNDNNEMVIEGYALKFDTWSENLGGFKETISRRALENTDLSDVRCLVDHIPSQIIGRTKSGTLELETDDVGLKYRCKLPNTTFARDLYENMRVGNINQCSFGFMLDDKGDEVRFDEQENIYKRTLTAIRELTDVSVVTYPAYKDTDVKPALRSIETVKKEQRKKELEIRLKKHSILNNIW

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]