From AureoWiki
Jump to navigation Jump to search
PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02221
  • pan locus tag?: SAUPAN005172000
  • symbol: SAOUHSC_02221
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02221
  • symbol: SAOUHSC_02221
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2066531..2067310
  • length: 780
  • essential: no DEG

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACTGAAAAAACTAATCAAGATGTCGATATCTTAACGCAACTAGGTGTAAAAGACATC
    AGCAAACAAAATGCAAACAAGTTTTATAAATTTGCGATATACGGCAAGTTCGGGACTGGT
    AAAACTACGTTTTTAACAAAAGATAACAACGCCTTAGTACTAGATATAAATGAGGACGGA
    ACAACGGTAACAGAAGATGGGGCAGTTGTGCAGATTAAGAATTACAAGCATTTTAGTGCA
    GTGATTAAGATGTTACCTAAAATTATTGAACAACTCAGAGAAAACGGAAAACAAATTGAT
    GTTGTAGTGATTGAAACAATCCAAAAGCTACGTGATATCACTATGGACGACATCATGGAC
    GGAAAATTAAAGAAACCAACATTTAATGATTGGGGCGAGTGTGCTACACGCATTGTAAGT
    ATTTATCGTTATATTTCTAAATTACAAGAACATTATCAATTCCATCTTGCTATAAGTGGA
    CACGAGGGAATTAACAAAGACAAAGATGATGAGGGTAGCACTATCAATCCAACAATCACG
    ATAGAGGCACAAGATCAAATAAAAAAAGCGGTCATCAGTCAATCTGATGTGTTAGCAAGA
    ATGACAATAGAAGAACATGAGCAAGACGGCGAAAAAGCTTATCAATATGTTCTTAACGCT
    GAACCATCAAACTTATTCGAGACAAAGATAAGACACTCAAGCAACATTAAAATTAACAAC
    AAACGTTTCATTAATCCAAGTATTAACGACGTAGTACAAGCAATCAGAAATGGAAACTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_02221
  • symbol: SAOUHSC_02221
  • description: hypothetical protein
  • length: 259
  • theoretical pI: 6.52588
  • theoretical MW: 29438.2
  • GRAVY: -0.542085

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Phage phi 11 orf12 protein homolog
  • PFAM:
    P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 109.5)
    and 9 more
    Bac_DnaA; Bacterial dnaA protein (PF00308; HMM-score: 19.6)
    no clan defined TFIIE-A_C; C-terminal general transcription factor TFIIE alpha (PF11521; HMM-score: 17)
    TAP42; TAP42-like family (PF04177; HMM-score: 15.2)
    SpoIISA_toxin; Toxin SpoIISA, type II toxin-antitoxin system (PF14171; HMM-score: 14.4)
    P-loop_NTPase (CL0023) ATPase_2; ATPase domain predominantly from Archaea (PF01637; HMM-score: 14.3)
    AAA_22; AAA domain (PF13401; HMM-score: 12)
    ABC_tran; ABC transporter (PF00005; HMM-score: 11.9)
    TsaE; Threonylcarbamoyl adenosine biosynthesis protein TsaE (PF02367; HMM-score: 11.9)
    cobW; CobW/HypB/UreG, nucleotide-binding domain (PF02492; HMM-score: 11.4)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.036041
    • TAT(Tat/SPI): 0.002301
    • LIPO(Sec/SPII): 0.001162
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MTEKTNQDVDILTQLGVKDISKQNANKFYKFAIYGKFGTGKTTFLTKDNNALVLDINEDGTTVTEDGAVVQIKNYKHFSAVIKMLPKIIEQLRENGKQIDVVVIETIQKLRDITMDDIMDGKLKKPTFNDWGECATRIVSIYRYISKLQEHYQFHLAISGHEGINKDKDDEGSTINPTITIEAQDQIKKAVISQSDVLARMTIEEHEQDGEKAYQYVLNAEPSNLFETKIRHSSNIKINNKRFINPSINDVVQAIRNGN

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]