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NCBI: 01-MAR-2011 (discontinued in NCBI 03-AUG-2016)

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02240
  • pan locus tag?: SAUPAN005012000
  • symbol: SAOUHSC_02240
  • pan gene symbol?: hlb-2
  • synonym:
  • product: truncated beta-hemolysin

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02240
  • symbol: SAOUHSC_02240
  • product: truncated beta-hemolysin
  • replicon: chromosome
  • strand: +
  • coordinates: 2074631..2075455
  • length: 825
  • essential: no DEG other strains
  • comment: SAOUHSC_02240 is the N-terminal part of the hlb gene which is split by insertion of the Φ13 prophage; complete protein: UniProt Q2FWP1

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGTATCCAAACTGGGGGCAATATAAACGCGCTGATTTAATCGGACAATCTTCTTATATT
    AAAAATAATGATGTCGTAATATTCAATGAAGCATTTGATAATGGTGCATCAGACAAATTA
    TTAAGTAATGTGAAAAAAGAATATCCTTATCAAACACCTGTACTCGGCCGTTCTCAATCA
    GGGTGGGACAAAACTGAAGGTAGCTACTCATCAACTGTTGCAGAAGATGGTGGCGTAGCG
    ATTGTAAGTAAATATCCTATTAAAGAAAAAATCCAGCATGTTTTCAAAAGCGGTTGTGGA
    TTCGATAATGATAGCAACAAAGGCTTTGTTTATACAAAAATAGAGAAAAATGGTAAGAAC
    GTTCACGTTATCGGTACACATACACAATCTGAAGATTCACGTTGTGGTGCTGGACATGAT
    CGAAAAATTAGAGCTGAACAAATGAAAGAAATCAGTGATTTTGTTAAAAAGAAAAATATC
    CCTAAAGATGAAACGGTATATATAGGTGGCGACCTTAATGTCAATAAAGGCACTCCAGAG
    TTCAAAGATATGCTTAAAAACTTGAATGTAAATGATGTTCTATATGCAGGTCATAATAGC
    ACATGGGACCCTCAATCAAATTCAATTGCGAAATATAATTACCCTAATGGTAAACCAGAA
    CATTTAGACTATATATTTACAGATAAAGATCATAAACAACCAAAACAATTAGTCAATGAA
    GTTGTGACTGAAAAACCTAAGCCATGGGATGTATATGCGTTCCCATATTACTACGTTTAC
    AATGATTTTTCAGATCATTACCCAATCAAAGCCTATAGTAAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    825

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02240
  • symbol: SAOUHSC_02240
  • description: truncated beta-hemolysin
  • length: 274
  • theoretical pI: 8.15182
  • theoretical MW: 31255.6
  • GRAVY: -0.884672

Function[edit source | edit]

  • reaction:
    EC 3.1.4.3?  ExPASy
    Phospholipase CA phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine
  • TIGRFAM:
    Cellular processesCellular processesPathogenesissphingomyelin phosphodiesterase (TIGR03395; EC 3.1.4.12; HMM-score: 383.3)
  • TheSEED:  
    Beta-hemolysin 
  • PFAM:
    DNase_I-like (CL0530) Exo_endo_phos; Endonuclease/Exonuclease/phosphatase family (PF03372; HMM-score: 54)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.127
    • Ymax_pos: 37
    • Cmax: 0.117
    • Cmax_pos: 37
    • Smax: 0.193
    • Smax_pos: 27
    • Smean: 0.108
    • D: 0.12
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MYPNWGQYKRADLIGQSSYIKNNDVVIFNEAFDNGASDKLLSNVKKEYPYQTPVLGRSQSGWDKTEGSYSSTVAEDGGVAIVSKYPIKEKIQHVFKSGCGFDNDSNKGFVYTKIEKNGKNVHVIGTHTQSEDSRCGAGHDRKIRAEQMKEISDFVKKKNIPKDETVYIGGDLNVNKGTPEFKDMLKNLNVNDVLYAGHNSTWDPQSNSIAKYNYPNGKPEHLDYIFTDKDHKQPKQLVNEVVTEKPKPWDVYAFPYYYVYNDFSDHYPIKAYSK

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Mark J J B Sibbald, Theresa Winter, Magdalena M van der Kooi-Pol, G Buist, E Tsompanidou, Tjibbe Bosma, Tina Schäfer, Knut Ohlsen, Michael Hecker, Haike Antelmann, Susanne Engelmann, Jan Maarten van Dijl
Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus.
J. Bacteriol.: 2010, 192(14);3788-800
[PubMed:20472795] [WorldCat.org] [DOI] (I p)
K Dziewanowska, V M Edwards, J R Deringer, G A Bohach, D J Guerra
Comparison of the beta-toxins from Staphylococcus aureus and Staphylococcus intermedius.
Arch. Biochem. Biophys.: 1996, 335(1);102-8
[PubMed:8914839] [WorldCat.org] [DOI] (P p)