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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02277
  • pan locus tag?: SAUPAN005298000
  • symbol: SAOUHSC_02277
  • pan gene symbol?: gcp
  • synonym:
  • product: DNA-binding/iron metalloprotein/AP endonuclease

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02277
  • symbol: SAOUHSC_02277
  • product: DNA-binding/iron metalloprotein/AP endonuclease
  • replicon: chromosome
  • strand: -
  • coordinates: 2108736..2109761
  • length: 1026
  • essential: yes [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGACTAAAGATATATTAATACTAGCTGTTGAAACAAGTTGTGATGAAACAAGCGTTAGT
    GTTATAAAAAATGGCAGAGATATTTTATCAAATACAGTTTTAAGTCAGATTGAAAGTCAT
    AAACGATTTGGCGGTGTCGTTCCCGAAGTGGCAAGTAGACATCACGTTGAAGGTATAACA
    GCAACAATAAACGAGGCTCTAGGGGATGCCGATGTATCAATAGAAGATATTGATGCCATA
    GCGGTTACAGAAGGCCCTGGACTAATTGGTGCGTTACTAATAGGTGTTAATGCAGCCAAA
    GCATTGGCATTTGCTTACGATAAGCCACTTATTCCTGTTCATCATATTGCAGGACATATA
    TATGCTAATCACATAGAAGAGCCATTAACATTCCCGCTAATTGCACTTATTGTTTCAGGT
    GGACATACTGAATTAGTTTATATGAAGGATCATTTATCATTTGAAGTCATTGGTGAAACA
    CGAGATGACGCAGTAGGTGAGGCTTATGATAAAGTGGCACGAACAATTGGTTTAAATTAT
    CCAGGTGGTCCACAAGTTGATCGGTTGGCTGCTGAAGGTGAAGATACTTATTCATTCCCT
    CGTGTTTGGTTGGATAAAGATAGTTATGATTTTAGTTTTAGTGGGTTGAAAAGTGCCGTA
    ATCAATCAACTTCACAATCAACGACAAAAAAATATTCCAATCATTGAAGCTAACGTAGCA
    ACGAGCTTTCAAAACAGTGTTGTAGAGGTGCTCACGTTTAAAGCTATTCAAGCTTGTAAA
    GAATATGGTGTTCAGCGATTAATTGTTGCTGGTGGCGTGGCGAGTAATAAAGGATTACGT
    CAATCTTTAGCGGATCAATGCAAAGTCAATGACATTCAATTAACTATCCCAAGTCCTAAA
    TTATGCACAGATAATGCTGCAATGATAGGCGTTGCCGGCCACTATTTGTATCAGCAAGGT
    CGATTTGCTGATTTAGCATTAAATGGGCACAGCAATATAGATTTAGAAGAGTATTCTGCA
    GAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1026

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02277
  • symbol: SAOUHSC_02277
  • description: DNA-binding/iron metalloprotein/AP endonuclease
  • length: 341
  • theoretical pI: 4.7955
  • theoretical MW: 36818.4
  • GRAVY: 0.00469208

Function[edit source | edit]

  • reaction:
    EC 3.4.24.57?  ExPASy
    O-sialoglycoprotein endopeptidaseHydrolysis of O-sialoglycoproteins; cleaves 31-Arg-|-Asp-32 bond in glycophorin A. Does not cleave unglycosylated proteins, desialylated glycoproteins or glycoproteins that are only N-glycosylated
    EC 2.3.1.234?  ExPASy
    N6-L-threonylcarbamoyladenine synthaseL-threonylcarbamoyladenylate + adenine37 in tRNA = AMP + N6-L-threonylcarbamoyladenine37 in tRNA
  • TIGRFAM:
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA threonylcarbamoyl adenosine modification protein TsaD (TIGR03723; HMM-score: 439.8)
    Genetic information processingProtein fateDegradation of proteins, peptides, and glycopeptidesmetallohydrolase, glycoprotease/Kae1 family (TIGR00329; HMM-score: 364.9)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairuniversal archaeal protein Kae1 (TIGR03722; HMM-score: 223.5)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA threonylcarbamoyl adenosine modification protein YeaZ (TIGR03725; HMM-score: 57.6)
    Genetic information processingProtein fateProtein modification and repaircarbamoyltransferase HypF (TIGR00143; HMM-score: 18.2)
  • TheSEED:  
    Cell Division and Cell CycleCell Division and Cell Cycle - no subcategoryYgjD and YeaZ TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA 
    RNA MetabolismRNA processing and modificationYrdC-YciO TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA 
    RNA processing and modificationYrdC-YciO-Sua5 and associated protein families TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA 
    Bacterial RNA-metabolizing Zn-dependent hydrolases TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA 
    Conserved gene cluster associated with Met-tRNA formyltransferase TsaD/Kae1/Qri7 protein, required for threonylcarbamoyladenosine t(6)A37 formation in tRNA 
  • PFAM:
    Actin_ATPase (CL0108) Peptidase_M22; Glycoprotease family (PF00814; HMM-score: 344.7)
    Carbam_trans_N; Carbamoyltransferase N-terminus (PF02543; HMM-score: 20.8)
    FGGY_C; FGGY family of carbohydrate kinases, C-terminal domain (PF02782; HMM-score: 13.8)
    NADP_Rossmann (CL0063) DUF166; Domain of unknown function (PF02593; HMM-score: 13.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Fe2+
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.136
    • Ymax_pos: 21
    • Cmax: 0.109
    • Cmax_pos: 36
    • Smax: 0.209
    • Smax_pos: 9
    • Smean: 0.153
    • D: 0.142
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFGGVVPEVASRHHVEGITATINEALGDADVSIEDIDAIAVTEGPGLIGALLIGVNAAKALAFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGETRDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKSAVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKEYGVQRLIVAGGVASNKGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNIDLEEYSAE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Li Zheng, Junshu Yang, Christina Landwehr, Frank Fan, Yinduo Ji
Identification of an essential glycoprotease in Staphylococcus aureus.
FEMS Microbiol. Lett.: 2005, 245(2);279-85
[PubMed:15837383] [WorldCat.org] [DOI] (P p)
Li Zheng, Chuanxin Yu, Kenneth Bayles, Iñigo Lasa, Yinduo Ji
Conditional mutation of an essential putative glycoprotease eliminates autolysis in Staphylococcus aureus.
J. Bacteriol.: 2007, 189(7);2734-42
[PubMed:17237169] [WorldCat.org] [DOI] (P p)