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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02288
  • pan locus tag?: SAUPAN005310000
  • symbol: leuD
  • pan gene symbol?: leuD
  • synonym:
  • product: 3-isopropylmalate dehydratase small subunit

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02288
  • symbol: leuD
  • product: 3-isopropylmalate dehydratase small subunit
  • replicon: chromosome
  • strand: +
  • coordinates: 2120679..2121251
  • length: 573
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGGCAGCAATCAAACCTATTACAACATATAAAGGTAAAATAGTCCCTCTCTTCAACGAC
    AATATCGATACAGACCAAATCATTCCTAAGGTACACTTAAAGCGTATTTCAAAAAGTGGC
    TTTGGTCCATTTGCTTTTGATGAATGGCGGTACTTACCTGATGGTTCAGATAATCCTGAT
    TTCAATCCTAACAAACCACAATATAAAGGGGCTTCTATTTTAATTACTGGAGATAATTTT
    GGATGTGGTTCAAGTCGTGAACATGCTGCTTGGGCTCTTAAGGACTATGGTTTTCATATT
    ATTATTGCAGGAAGTTTCAGTGACATATTTTATATGAATTGCACTAAAAATGCGATGTTG
    CCTATCGTTTTAGAAAAAAGTGCCCGTGAACATCTTGCACAATATGTTGAAATTGAGGTC
    GATTTACCAAATCAAACTGTGTCATCACCAGACAAGCGTTTCCATTTTGAAATTGATGAA
    ACTTGGAAGAATAAACTTGTAAATGGCTTAGATGACATTGCAATCACCCTACAATATGAA
    TCATTAATAGAAAAATATGAAAAATCACTTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    573

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02288
  • symbol: LeuD
  • description: 3-isopropylmalate dehydratase small subunit
  • length: 190
  • theoretical pI: 5.63012
  • theoretical MW: 21578.4
  • GRAVY: -0.356842

Function[edit source | edit]

  • reaction:
    EC 4.2.1.33?  ExPASy
    3-isopropylmalate dehydratase(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
  • TIGRFAM:
    MetabolismAmino acid biosynthesisPyruvate family3-isopropylmalate dehydratase, small subunit (TIGR00171; EC 4.2.1.33; HMM-score: 223.5)
    3-isopropylmalate dehydratase, small subunit (TIGR02087; HMM-score: 83.1)
    MetabolismAmino acid biosynthesisPyruvate family3-isopropylmalate dehydratase, small subunit (TIGR02084; EC 4.2.1.33; HMM-score: 80)
    MetabolismEnergy metabolismTCA cycleputative aconitate hydratase (TIGR01342; EC 4.2.1.3; HMM-score: 48.1)
    MetabolismAmino acid biosynthesisAspartate familyhomoaconitase (TIGR00139; EC 4.2.1.36; HMM-score: 40.1)
    MetabolismEnergy metabolismTCA cycleaconitate hydratase, mitochondrial (TIGR01340; EC 4.2.1.3; HMM-score: 29)
    MetabolismEnergy metabolismTCA cycleaconitate hydratase 1 (TIGR01341; EC 4.2.1.3; HMM-score: 27.6)
    2-methylisocitrate dehydratase, Fe/S-dependent (TIGR02333; EC 4.2.1.99; HMM-score: 17.2)
  • TheSEED:  
    Amino Acids and DerivativesBranched-chain amino acidsBranched-Chain Amino Acid Biosynthesis 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) 
    Branched-chain amino acidsLeucine Biosynthesis 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) 
  • PFAM:
    Leu-IlvD (CL0364) Aconitase_C; Aconitase C-terminal domain (PF00694; HMM-score: 115.9)
    Aconitase_2_N; Aconitate hydratase 2 N-terminus (PF06434; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.163
    • Ymax_pos: 11
    • Cmax: 0.11
    • Cmax_pos: 3
    • Smax: 0.435
    • Smax_pos: 1
    • Smean: 0.222
    • D: 0.186
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPFAFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIIIAGSFSDIFYMNCTKNAMLPIVLEKSAREHLAQYVEIEVDLPNQTVSSPDKRFHFEIDETWKNKLVNGLDDIAITLQYESLIEKYEKSL

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: SAOUHSC_01228 (CodY*)
    CodY* (TF) important in Amino acid metabolism  RegPrecise    transcription unit transferred from N315 data RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]