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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02299
  • pan locus tag?: SAUPAN005334000
  • symbol: SAOUHSC_02299
  • pan gene symbol?: rsbW
  • synonym:
  • product: serine-protein kinase RsbW

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02299
  • symbol: SAOUHSC_02299
  • product: serine-protein kinase RsbW
  • replicon: chromosome
  • strand: -
  • coordinates: 2132674..2133183
  • length: 510
  • essential: no [1] DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTTAATGAAGGAACGGAGGTCGAATAACATGCAATCTAAAGAAGATTTTATCGAAATG
    CGCGTGCCAGCATCGGCAGAGTATGTAAGTTTAATTCGTTTAACACTTTCTGGCGTTTTT
    TCGAGAGCTGGTGCTACATATGATGATATTGAAGATGCCAAGATTGCAGTTAGTGAAGCT
    GTGACAAATGCAGTTAAACATGCATACAAAGAAAATAACAATGTGGGCATTATTAACATA
    TATTTTGAAATTTTAGAAGATAAAATTAAAATTGTTATTTCTGATAAAGGTGACAGTTTT
    GATTATGAAACAACTAAATCAAAAATAGGTCCTTACGATAAAGACGAAAATATAGACTTT
    TTACGCGAAGGTGGCCTAGGTTTATTTTTAATCGAATCTTTAATGGATGAAGTCACAGTA
    TATAAAGAATCTGGTGTGACAATCAGTATGACTAAGTATATAAAAAAAGAGCAGGTGCGA
    AATAATGGCGAAAGAGTCGAAATCAGCTAA
    60
    120
    180
    240
    300
    360
    420
    480
    510

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02299
  • symbol: SAOUHSC_02299
  • description: serine-protein kinase RsbW
  • length: 169
  • theoretical pI: 4.63512
  • theoretical MW: 19181.6
  • GRAVY: -0.388166

Function[edit source | edit]

  • reaction:
    EC 2.7.11.1?  ExPASy
    Non-specific serine/threonine protein kinaseATP + a protein = ADP + a phosphoprotein
  • TIGRFAM:
    anti-sigma B factor (TIGR01924; EC 2.7.11.1; HMM-score: 271.9)
    Cellular processesCellular processesSporulation and germinationanti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 63.5)
    Signal transductionRegulatory functionsProtein interactionsanti-sigma F factor (TIGR01925; EC 2.7.11.1; HMM-score: 63.5)
  • TheSEED:  
    SigmaB stress responce regulation Serine-protein kinase RsbW (EC 2.7.11.1) 
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 94.4)
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 26)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.24
    • Ymax_pos: 46
    • Cmax: 0.267
    • Cmax_pos: 46
    • Smax: 0.361
    • Smax_pos: 41
    • Smean: 0.18
    • D: 0.217
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLMKERRSNNMQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAKIAVSEAVTNAVKHAYKENNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPYDKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor: SAOUHSC_02298 (SigB*) [4] other strains
    SigB* (sigma factor) controls a large regulon involved in stress/starvation response and adaptation
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Markus Bischoff, Paul Dunman, Jan Kormanec, Daphne Macapagal, Ellen Murphy, William Mounts, Brigitte Berger-Bächi, Steven Projan
    Microarray-based analysis of the Staphylococcus aureus sigmaB regulon.
    J. Bacteriol.: 2004, 186(13);4085-99
    [PubMed:15205410] [WorldCat.org] [DOI] (P p)
  5. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Kullik, Giachino
The alternative sigma factor sigmaB in Staphylococcus aureus: regulation of the sigB operon in response to growth phase and heat shock
Arch. Microbiol.: 1997, 167(2/3);151-9
[PubMed:9042755] [WorldCat.org] (I p)