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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02362
  • pan locus tag?: SAUPAN005413000
  • symbol: rho
  • pan gene symbol?: rho
  • synonym:
  • product: transcription termination factor Rho

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02362
  • symbol: rho
  • product: transcription termination factor Rho
  • replicon: chromosome
  • strand: -
  • coordinates: 2182974..2184290
  • length: 1317
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGCCTGAAAGAGAACGTACATCTCCTCAGTATGAATCATTCCACGAATTGTACAAGAAC
    TATACTACCAAGGAACTCACTCAAAAAGCTAAAACTCTTAAGTTGACGAACTATAGTAAA
    TTAAATAAAAAAGAACTTGTTCTAGCTATTATGGAAGCACAAATGGAAAAAGATGGTAAC
    TATTATATGGAAGGTATCTTAGATGATATACAACCAGATGGTTATGGTTTTTTAAGAACA
    GTGAACTATTCTAAAGGGGAAAAAGATATTTATATATCTGCTAGCCAAATTCGTCGTTTT
    GAAATTAAACGTGGGGATAAAGTAACTGGGAAAGTTAGAAAACCTAAAGATAACGAAAAA
    TATTATGGCTTATTACAAGTTGACTTTGTCAATGACCATAACGCAGAAGAAGTGAAGAAA
    CGTCCGCATTTCCAAGCTTTGACACCACTTTATCCAGATGAGCGTATTAAATTAGAGACA
    GAAATACAAAATTATTCAACGCGCATCATGGATTTAGTAACACCGATTGGTTTAGGTCAA
    CGTGGTTTAATAGTGGCGCCACCTAAAGCAGGTAAAACATCGTTATTAAAAGAAATAGCG
    AATGCAATCAGTACGAACAAACCAGATGCAAAGCTATTTATTTTGTTAGTTGGCGAGCGT
    CCTGAAGAGGTAACAGATTTAGAACGCTCAGTAGAAGCTGCTGAAGTCGTTCATTCAACG
    TTTGACGAACCACCAGAACACCATGTTAAAGTAGCTGAATTATTACTTGAACGTGCAAAG
    CGTTTAGTAGAAATTGGGGAAGATGTCATTATTTTAATGGATTCTATAACGAGATTAGCA
    CGCGCTTATAACTTAGTTATTCCACCAAGTGGTCGTACATTATCAGGTGGTTTAGATCCT
    GCATCTTTACACAAACCAAAAGCATTCTTCGGTGCAGCGAGAAATATTGAAGCGGGTGGA
    AGTTTAACAATACTTGCAACTGCATTAGTTGATACGGGTTCACGTATGGACGATATGATT
    TACGAAGAATTTAAAGGAACAGGTAACATGGAGTTACATTTAGATCGTAAATTGTCTGAA
    CGTCGTATCTTCCCTGCAATTGATATTGGCAGAAGTTCAACGCGTAAAGAAGAATTGTTG
    ATAAGTAAATCTGAATTAGACACATTATGGCAATTAAGAAATCTATTCACTGACTCAACT
    GACTTTACTGAAAGATTTATTCGCAAACTTAAAAGGTCTAAGAATAATGAAGATTTCTTC
    AAGCAGCTACAAAAGTCTGCAGAAGAAAGTACTAAAACGGGTCGACCTATAATTTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1317

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02362
  • symbol: Rho
  • description: transcription termination factor Rho
  • length: 438
  • theoretical pI: 8.53546
  • theoretical MW: 49969.8
  • GRAVY: -0.569406

Function[edit source | edit]

  • reaction:
    EC 3.6.4.-?  ExPASy
  • TIGRFAM:
    Genetic information processingTranscriptionTranscription factorstranscription termination factor Rho (TIGR00767; EC 3.6.4.-; HMM-score: 620.5)
    MetabolismEnergy metabolismATP-proton motive force interconversionATPase, FliI/YscN family (TIGR01026; EC 3.6.3.14; HMM-score: 85.1)
    Cellular processesCellular processesChemotaxis and motilityflagellar protein export ATPase FliI (TIGR03496; EC 3.6.3.14; HMM-score: 84.7)
    flagellar protein export ATPase FliI (TIGR03498; EC 3.6.3.14; HMM-score: 84.3)
    Cellular processesCellular processesPathogenesistype III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 84)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingtype III secretion apparatus H+-transporting two-sector ATPase (TIGR02546; EC 3.6.3.14; HMM-score: 84)
    Cellular processesCellular processesChemotaxis and motilityflagellar protein export ATPase FliI (TIGR03497; EC 3.6.3.14; HMM-score: 74.7)
    MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase F1, beta subunit (TIGR01039; EC 3.6.3.14; HMM-score: 38.4)
    alternate F1F0 ATPase, F1 subunit alpha (TIGR03324; EC 3.6.3.-; HMM-score: 33)
    alternate F1F0 ATPase, F1 subunit beta (TIGR03305; EC 3.6.3.-; HMM-score: 27)
    Cellular processesCellular processesSporulation and germinationstage III sporulation protein AA (TIGR02858; HMM-score: 25.3)
    MetabolismEnergy metabolismATP-proton motive force interconversionATP synthase F1, alpha subunit (TIGR00962; EC 3.6.3.14; HMM-score: 19.9)
  • TheSEED:  
    RNA MetabolismTranscriptionTranscription factors bacterial Transcription termination factor Rho 
  • PFAM:
    OB (CL0021) Rho_RNA_bind; Rho termination factor, RNA-binding domain (PF07497; HMM-score: 98.1)
    P-loop_NTPase (CL0023) ATP-synt_ab; ATP synthase alpha/beta family, nucleotide-binding domain (PF00006; HMM-score: 75.6)
    HeH (CL0306) Rho_N; Rho termination factor, N-terminal domain (PF07498; HMM-score: 44.3)
    P-loop_NTPase (CL0023) NACHT; NACHT domain (PF05729; HMM-score: 25.2)
    OB (CL0021) OB_RNB; Ribonuclease B OB domain (PF08206; HMM-score: 21.2)
    P-loop_NTPase (CL0023) AAA; ATPase family associated with various cellular activities (AAA) (PF00004; HMM-score: 16)
    AAA_25; AAA domain (PF13481; HMM-score: 15.8)
    HTH (CL0123) Mor; Mor transcription activator family (PF08765; HMM-score: 14.8)
    P-loop_NTPase (CL0023) AAA_24; AAA domain (PF13479; HMM-score: 13.5)
    AAA_14; AAA domain (PF13173; HMM-score: 12.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.114
    • Ymax_pos: 55
    • Cmax: 0.104
    • Cmax_pos: 42
    • Smax: 0.184
    • Smax_pos: 48
    • Smean: 0.099
    • D: 0.108
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKKELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEIKRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLETEIQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVGERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSITRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGSRMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQLRNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

R S Washburn, A Marra, A P Bryant, M Rosenberg, D R Gentry
rho is not essential for viability or virulence in Staphylococcus aureus.
Antimicrob. Agents Chemother.: 2001, 45(4);1099-103
[PubMed:11257021] [WorldCat.org] [DOI] (P p)