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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02375
  • pan locus tag?: SAUPAN005430000
  • symbol: SAOUHSC_02375
  • pan gene symbol?: luxS
  • synonym:
  • product: S-ribosylhomocysteinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02375
  • symbol: SAOUHSC_02375
  • product: S-ribosylhomocysteinase
  • replicon: chromosome
  • strand: +
  • coordinates: 2198622..2199092
  • length: 471
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGACAAAAATGAATGTTGAAAGTTTCAATTTAGATCATACTAAGGTGGTTGCCCCATTT
    ATTAGATTAGCGGGAACGATGGAAGGATTAAACGGAGATGTCATTCACAAATACGACATT
    CGTTTCAAACAACCAAACAAAGAACATATGGATATGCCCGGACTACATTCATTAGAACAT
    TTAATGGCTGAAAATATTAGAAATCATAGTGACAAAGTTGTTGATTTAAGTCCTATGGGT
    TGCCAAACTGGTTTCTATGTATCATTTATTAATCATGATAATTATGATGATGTATTAAAT
    ATTGTTGAAGCAACTTTAAATGATGTGCTAAATGCTACTGAAGTGCCTGCTTGTAATGAA
    GTACAATGTGGCTGGGCAGCAAGTCATTCATTAGAAGGTGCTAAAACTATCGCTCAAGCA
    TTTCTAGACAAACGAAACGAATGGCATGATGTTTTCGGTACAGGAAAATAA
    60
    120
    180
    240
    300
    360
    420
    471

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02375
  • symbol: SAOUHSC_02375
  • description: S-ribosylhomocysteinase
  • length: 156
  • theoretical pI: 5.45928
  • theoretical MW: 17513.7
  • GRAVY: -0.355128

Function[edit source | edit]

  • reaction:
    EC 4.4.1.21?  ExPASy
    S-ribosylhomocysteine lyaseS-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione
  • TIGRFAM:
  • TheSEED:  
    Amino Acids and DerivativesLysine, threonine, methionine, and cysteineMethionine Biosynthesis S-ribosylhomocysteine lyase (EC 4.4.1.21) 
    and 2 more
    Lysine, threonine, methionine, and cysteineMethionine Degradation S-ribosylhomocysteine lyase (EC 4.4.1.21) 
    Methionine Biosynthesis Autoinducer-2 production protein LuxS 
  • PFAM:
    Peptidase_ME (CL0094) LuxS; S-Ribosylhomocysteinase (LuxS) (PF02664; HMM-score: 186.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors: Fe cation
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.146
    • Ymax_pos: 17
    • Cmax: 0.12
    • Cmax_pos: 33
    • Smax: 0.266
    • Smax_pos: 11
    • Smean: 0.208
    • D: 0.17
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQPNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFINHDNYDDVLNIVEATLNDVLNATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_02375 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator: CymR* (repression) regulon
    CymR(TF)important in Cysteine metabolism;RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Neil Doherty, Matthew T G Holden, Saara N Qazi, Paul Williams, Klaus Winzer
Functional analysis of luxS in Staphylococcus aureus reveals a role in metabolism but not quorum sensing.
J. Bacteriol.: 2006, 188(8);2885-97
[PubMed:16585750] [WorldCat.org] [DOI] (P p)