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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02385
  • pan locus tag?: SAUPAN005440000
  • symbol: SAOUHSC_02385
  • pan gene symbol?: manA
  • synonym:
  • product: mannose-6-phosphate isomerase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02385
  • symbol: SAOUHSC_02385
  • product: mannose-6-phosphate isomerase
  • replicon: chromosome
  • strand: -
  • coordinates: 2206816..2207673
  • length: 858
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGATACAACTGGGGGAATGTTGGTGTGTGTCAGCACATCCAAATGGTACGAGCGAGATT
    ATTAATGGACCATATCAAGGTCAAACATTAGACCGTATTTGGTCAGAACATCGTGAATTG
    TTTGGTGATTTCCCAAGCAAAGATTTTCCGCTTCTAACTAAAATAGTGGATGCAAGAGAA
    TCACTTTCTATTCATGTGCACCCTGATAATTCTTATGCTTATGAGCATGAAAACGGGCAA
    TATGGCAAATCTGAATGTTGGTATATTATAGATGCAGAAGAAGATGCAGAAATAGTTATA
    GGGACATTAGCAGAGTCTAGAGAAGAAGTTGCGAATCATGTTCAACACGGAACGATAGAG
    TCGATACTTAGATATATTAAAGTAAAACCTGGAGAATTCTATTTTATTCCAGCAGGAACA
    GTACATACTATTTCTTCAGGAATATTAGCATACGAAACGATGCAATCGTCAGACATTACA
    TATAGACTTTATGATTTCAATCGTCAAGATAATCAATATAATGATAGACCGTTAAATATT
    GAAAAAGCTTTAGACGTTATTCAGTACAATGCACCATTACCTAATATTTTGCCTGAAAGC
    GAAATTATTGAAAACCATAAGTGTACACACATTGTATCGAATGATTTCTTTACATTGGTT
    AAATGGGAAATTTCTGGCACGTTAAATTATATGAAGCCTAGAGAGTTCTGTTTAGTTACA
    GTGTTGGAAGGCGAAGGGCAAATGATTGTCGATGGTGAAATTTTCAAACTGACTACTGGT
    ACAAACTTTATTTTGACTTCTGAAGATTTGGATAGTGTCTTTGAAGGTGATTTCACATTG
    ATGATTAGCTATGTGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    858

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02385
  • symbol: SAOUHSC_02385
  • description: mannose-6-phosphate isomerase
  • length: 285
  • theoretical pI: 4.29057
  • theoretical MW: 32535.2
  • GRAVY: -0.274737

Function[edit source | edit]

  • reaction:
    EC 5.3.1.8?  ExPASy
    Mannose-6-phosphate isomeraseD-mannose 6-phosphate = D-fructose 6-phosphate
  • TIGRFAM:
    MetabolismEnergy metabolismSugarsmannose-6-phosphate isomerase, class I (TIGR00218; EC 5.3.1.8; HMM-score: 177.5)
  • TheSEED:  
    CarbohydratesMonosaccharidesMannose Metabolism Mannose-6-phosphate isomerase, class I (EC 5.3.1.8) 
  • PFAM:
    Cupin (CL0029) PMI_typeI; Phosphomannose isomerase type I (PF01238; HMM-score: 88.5)
    Cupin_2; Cupin domain (PF07883; HMM-score: 29.4)
    AraC_binding; AraC-like ligand binding domain (PF02311; HMM-score: 17.6)
    JmjC; JmjC domain, hydroxylase (PF02373; HMM-score: 17.5)
    Cupin_3; Protein of unknown function (DUF861) (PF05899; HMM-score: 12.2)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.126
    • Ymax_pos: 28
    • Cmax: 0.156
    • Cmax_pos: 28
    • Smax: 0.158
    • Smax_pos: 19
    • Smean: 0.101
    • D: 0.117
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MIQLGECWCVSAHPNGTSEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYEHENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEFYFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNAPLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMIVDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]