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NCBI: 03-AUG-2016

Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02454
  • pan locus tag?: SAUPAN005591000
  • symbol: SAOUHSC_02454
  • pan gene symbol?: lacB
  • synonym:
  • product: galactose-6-phosphate isomerase subunit LacB

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02454
  • symbol: SAOUHSC_02454
  • product: galactose-6-phosphate isomerase subunit LacB
  • replicon: chromosome
  • strand: -
  • coordinates: 2278565..2279080
  • length: 516
  • essential: no DEG other strains

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAGATTGCATTAGGATGCGACCATATTGTTACAGATACAAAAATGCGTGTATCTGAA
    TTTTTAAAATCAAAAGGACATGAAGTCATTGACGTAGGAACATACGATTTCACAAGAACA
    CATTATCCAATTTTTGGTAAAAAAGTTGGCGAACAAGTTGTTAGCGGTAATGCAGACTTA
    GGTGTTTGTATTTGTGGAACAGGTGTTGGTATTAACAATGCTGTAAATAAAGTACCTGGC
    GTTCGTTCAGCACTAGTACGTGATATGACATCAGCGTTATACGCTAAAGAAGAATTAAAT
    GCGAACGTTATTGGCTTCGGTGGACGTATTATAGGTGAGTTATTAATGTGCGATATTATC
    GATGCATTTATTAATGCTGAATATAAACCAACTGAAGAGAACAAAAAATTAATCGCTAAA
    ATTAAACATTTAGAAACAAGCAATGCAGATCAAGCTGATCCACATTTCTTTGATGAATTC
    TTAGAAAAATGGGACAGAGGCGAATACCACGATTAA
    60
    120
    180
    240
    300
    360
    420
    480
    516

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAOUHSC_02454
  • symbol: SAOUHSC_02454
  • description: galactose-6-phosphate isomerase subunit LacB
  • length: 171
  • theoretical pI: 5.59496
  • theoretical MW: 18950.5
  • GRAVY: -0.212866

Function[edit | edit source]

  • reaction:
    EC 5.3.1.26?  ExPASy
    Galactose-6-phosphate isomerase D-galactose 6-phosphate = D-tagatose 6-phosphate
  • TIGRFAM:
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides galactose-6-phosphate isomerase, LacB subunit (TIGR01119; EC 5.3.1.26; HMM-score: 375.9)
    and 5 more
    sugar-phosphate isomerase, RpiB/LacA/LacB family (TIGR00689; EC 5.3.1.-; HMM-score: 124.7)
    Metabolism Energy metabolism Pentose phosphate pathway ribose 5-phosphate isomerase B (TIGR01120; EC 5.3.1.6; HMM-score: 117.9)
    ribose 5-phosphate isomerase (TIGR02133; EC 5.3.1.6; HMM-score: 106)
    Metabolism Energy metabolism Biosynthesis and degradation of polysaccharides galactose-6-phosphate isomerase, LacA subunit (TIGR01118; EC 5.3.1.26; HMM-score: 48.8)
    Hypothetical proteins Conserved conserved hypothetical protein EF_0830/AHA_3911 (TIGR03577; HMM-score: 13.5)
  • TheSEED  :
    • Galactose-6-phosphate isomerase, LacB subunit (EC 5.3.1.26)
    Carbohydrates Di- and oligosaccharides Lactose and Galactose Uptake and Utilization  Galactose-6-phosphate isomerase, LacB subunit (EC 5.3.1.26)
  • PFAM:
    no clan defined LacAB_rpiB; Ribose/Galactose Isomerase (PF02502; HMM-score: 142.9)
    and 3 more
    Gly_rich_SFCGS; Glycine-rich SFCGS (PF14272; HMM-score: 16.5)
    Succ_CoA_synth (CL0506) Ligase_CoA; CoA-ligase (PF00549; HMM-score: 13.3)
    no clan defined Ku_PK_bind; Ku C terminal domain like (PF08785; HMM-score: 12.3)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.05171
    • TAT(Tat/SPI): 0.000494
    • LIPO(Sec/SPII): 0.002331
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPIFGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNANVIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEFLEKWDRGEYHD

Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Regulation[edit | edit source]

  • regulator: LacR* (repression) regulon
    LacR*(TF)important in Lactose utilization; transcription unit transferred from N315 data RegPrecise  [3]

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

  1. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit | edit source]

E L Rosey, G C Stewart
The nucleotide sequence of the lacC and lacD genes of Staphylococcus aureus.
Nucleic Acids Res: 1989, 17(10);3980
[PubMed:2543958] [WorldCat.org] [DOI] (P p)