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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02532
  • pan locus tag?: SAUPAN005724000
  • symbol: SAOUHSC_02532
  • pan gene symbol?: sarV
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02532
  • symbol: SAOUHSC_02532
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2335005..2335355
  • length: 351
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    ATGAGTAATAAAGTTCAACGTTTTATAGAAGCAGAAAGGGAGTTAAGTCAGTTAAAGCAC
    TGGTTAAAAACAACACATAAGATTTCAATTGAAGAATTTGTAGTCCTTTTTAAAGTGTAT
    GAAGCTGAAAAGATTAGCGGTAAAGAATTGAGGGATACATTACATTTTGAAATGCTATGG
    GATACAAGTAAAATCGATGTGATTATCCGTAAAATCTATAAAAAAGAGCTTATTTCTAAA
    TTGCGTTCTGAAACGGATGAAAGACAAGTATTCTATTTCTATAGTACTTCTCAAAAGAAA
    TTGTTAGATAAAATTACTAAAGAAATAGAAGTGTTAAGCGTTACAAACTAA
    60
    120
    180
    240
    300
    351

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02532
  • symbol: SAOUHSC_02532
  • description: hypothetical protein
  • length: 116
  • theoretical pI: 9.64293
  • theoretical MW: 13986.2
  • GRAVY: -0.47069

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionRegulatory functionsDNA interactionsstaphylococcal accessory regulator family (TIGR01889; HMM-score: 86.5)
    MetabolismTransport and binding proteinsCations and iron carrying compoundscopper transport repressor, CopY/TcrY family (TIGR02698; HMM-score: 21.5)
    Signal transductionRegulatory functionsDNA interactionscopper transport repressor, CopY/TcrY family (TIGR02698; HMM-score: 21.5)
    homoprotocatechuate degradation operon regulator, HpaR (TIGR02337; HMM-score: 19.4)
    MetabolismPurines, pyrimidines, nucleosides, and nucleotides2'-Deoxyribonucleotide metabolismnrdI protein (TIGR00333; HMM-score: 13.8)
  • TheSEED:  
    Transcriptional regulator SarV (Staphylococcal accessory regulator V) 
  • PFAM:
    HTH (CL0123) MarR; MarR family (PF01047; HMM-score: 26.5)
    Penicillinase_R; Penicillinase repressor (PF03965; HMM-score: 20.6)
    no clan definedSBDS; Shwachman-Bodian-Diamond syndrome (SBDS) protein (PF01172; HMM-score: 15.7)
    HTH (CL0123) HTH_27; Winged helix DNA-binding domain (PF13463; HMM-score: 15.5)
    GatB_YqeY (CL0279) GatB_Yqey; GatB domain (PF02637; HMM-score: 14.4)
    GT-B (CL0113) Glyco_transf_28; Glycosyltransferase family 28 N-terminal domain (PF03033; HMM-score: 14.1)
    no clan definedDUF4874; Domain of unknown function (DUF4874) (PF16173; HMM-score: 13.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.107
    • Ymax_pos: 11
    • Cmax: 0.112
    • Cmax_pos: 48
    • Smax: 0.153
    • Smax_pos: 4
    • Smean: 0.117
    • D: 0.111
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEKISGKELRDTLHFEMLWDTSKIDVIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLLDKITKEIEVLSVTN

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_02532 no polycistronic organisation predicted

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Adhar C Manna, Susham S Ingavale, MaryBeth Maloney, Willem van Wamel, Ambrose L Cheung
Identification of sarV (SA2062), a new transcriptional regulator, is repressed by SarA and MgrA (SA0641) and involved in the regulation of autolysis in Staphylococcus aureus.
J. Bacteriol.: 2004, 186(16);5267-80
[PubMed:15292128] [WorldCat.org] [DOI] (P p)