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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02777
  • pan locus tag?: SAUPAN006049000
  • symbol: SAOUHSC_02777
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02777
  • symbol: SAOUHSC_02777
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2553735..2554157
  • length: 423
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    ATGAACAAAAAACATGTTTTTATAATTATTGGTGTCATTTTATGTATATGTATAGTTGCA
    TCAGTCATTTATTTAAAAGTGAAATATGACGAAAAAGAAAAACAAAAAGCAATATACTAT
    AAAGAACAACAGGAGCGTATTACACTCTATCTTAAGCATAATACTAAAGAACCCAATACC
    ATCAAAACTGTACATTTCACAAGTTTAAAAAGAGGACCTATGGGCGATGCAGTAATTGAA
    GGTTACATAAATGAAAATAAAGAAGATGATTTTGTTGCATATGGGTCTCCAGAACATAAT
    TATCAATTTGGTGGAAGTTTAATCAAAAGTAAAAATTTAAGCACGTTATTAAAACCAGTA
    CATCAAACCAAATCACCTGATGAGATAAAAAAGGAATTAGAGTCTAAAAAGAACGACCGC
    TAA
    60
    120
    180
    240
    300
    360
    420
    423

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02777
  • symbol: SAOUHSC_02777
  • description: hypothetical protein
  • length: 140
  • theoretical pI: 9.61055
  • theoretical MW: 16201.6
  • GRAVY: -0.617143

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    MetabolismTransport and binding proteinsCarbohydrates, organic alcohols, and acidsD-galactonate transporter (TIGR00893; HMM-score: 15.7)
    Cellular processesCellular processesAdaptations to atypical conditionsphage shock protein B (TIGR02976; HMM-score: 13.2)
    MetabolismEnergy metabolismElectron transportcytochrome c oxidase, subunit II (TIGR02866; EC 1.9.3.1; HMM-score: 12.8)
  • TheSEED:  
    FIG01108221: hypothetical protein 
  • PFAM:
    no clan definedDUF1433; Protein of unknown function (DUF1433) (PF07252; HMM-score: 134.9)
    Viral_Beta_CD; Viral Beta C/D like family (PF04530; HMM-score: 15.7)
    Sec66; Preprotein translocase subunit Sec66 (PF09802; HMM-score: 14.5)
    DUF1310; Protein of unknown function (DUF1310) (PF07006; HMM-score: 13.8)
    DUF4500; Domain of unknown function (DUF4500) (PF14937; HMM-score: 12.5)
    Peptidase_AD (CL0130) Presenilin; Presenilin (PF01080; HMM-score: 11.3)
    ABC-2 (CL0181) ABC2_membrane_3; ABC-2 family transporter protein (PF12698; HMM-score: 10.7)
    no clan definedPMP1_2; ATPase proteolipid family (PF08114; HMM-score: 7.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helix: 1
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: 2
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.369
    • Ymax_pos: 30
    • Cmax: 0.348
    • Cmax_pos: 30
    • Smax: 0.585
    • Smax_pos: 18
    • Smean: 0.394
    • D: 0.379
  • predicted transmembrane helices (TMHMM): 1

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MNKKHVFIIIGVILCICIVASVIYLKVKYDEKEKQKAIYYKEQQERITLYLKHNTKEPNTIKTVHFTSLKRGPMGDAVIEGYINENKEDDFVAYGSPEHNYQFGGSLIKSKNLSTLLKPVHQTKSPDEIKKELESKKNDR

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]