From AureoWiki
Jump to: navigation, search
NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02801
  • pan locus tag?: SAUPAN006096000
  • symbol: SAOUHSC_02801
  • pan gene symbol?: gtaB
  • synonym:
  • product: UTP-glucose-1-phosphate uridylyltransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02801
  • symbol: SAOUHSC_02801
  • product: UTP-glucose-1-phosphate uridylyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2576833..2577615
  • length: 783
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCTTCCTATCTTAGATAAACCCACAATACAATATATCGTTGAAGAAGCTGCAAGAGCT
    GGAATTGAAGATATTATTATAGTGACAGGTCGCCACAAACGCGCGATTGAAGATCATTTT
    GATAGTCAAAAAGAATTAGAAATGGTGTTAAAAGAAAAAGGTAAATCTGAATTACTAGAG
    AAAGTTCAGTATTCAACGGAACTTGCGAATATTTTTTATGTAAGGCAGAAAGAACAAAAA
    GGTTTAGGGCATGCGATTAGTTCTGCGCGTCAATTTATCGGCAATGAACCATTTGCGGTA
    TTATTAGGTGACGATATTGTTGAATCTGAAGTGCCAGCAGTGAAACAGTTGATTGACGTT
    TATGAAGAAACAGGGCACTCAGTTATAGGTGTCCAAGAAGTTCCGGAAGCAGATACTCAT
    CGTTATGGAATTATTGACCCATTAACTAAAAACGGACGTCAGTATGAAGTGAAAAAGTTT
    GTTGAAAAGCCAGCACAAGGTACGGCACCATCAAACCTAGCTATTATGGGACGTTATGTA
    TTAACGCCTGAAATTTTCGATTATTTAAAAACGCAAAAAGAAGGCGCAGGTAACGAAATT
    CAGTTAACAGATGCGATTGAACGTATGAATAATGATAACCAAGTGTATGCATATGACTTC
    GAAGGCGAACGTTATGACGTTGGAGAAAAATTAGGCTTCGTTAAAACTACCATAGAATAT
    GCATTGAAAGATGACAGTATGCGAGAAGAATTAACACGATTTATTAAAGCATTAGGTTTA
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    783

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02801
  • symbol: SAOUHSC_02801
  • description: UTP-glucose-1-phosphate uridylyltransferase
  • length: 260
  • theoretical pI: 4.65139
  • theoretical MW: 29458.2
  • GRAVY: -0.425769

Function[edit source | edit]

  • reaction:
    EC 2.7.7.9?  ExPASy
    UTP--glucose-1-phosphate uridylyltransferaseUTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesUTP--glucose-1-phosphate uridylyltransferase (TIGR01099; EC 2.7.7.9; HMM-score: 340)
    Signal transductionRegulatory functionsProtein interactionsregulatory protein GalF (TIGR01105; HMM-score: 166.5)
    glucose-1-phosphate thymidylyltransferase (TIGR01208; EC 2.7.7.24; HMM-score: 114.4)
    UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR03992; EC 2.3.1.157,2.7.7.23; HMM-score: 109.9)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesglucose-1-phosphate thymidylyltransferase (TIGR01207; EC 2.7.7.24; HMM-score: 47.6)
    MetabolismEnergy metabolismBiosynthesis and degradation of polysaccharidesglucose-1-phosphate adenylyltransferase (TIGR02091; EC 2.7.7.27; HMM-score: 44)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesUDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 40.9)
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanUDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 40.9)
    MetabolismCentral intermediary metabolismAmino sugarsUDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase (TIGR01173; EC 2.3.1.157,2.7.7.23; HMM-score: 40.9)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesglucose-1-phosphate cytidylyltransferase (TIGR02623; EC 2.7.7.33; HMM-score: 37.7)
    Cell structureCell envelopeBiosynthesis and degradation of surface polysaccharides and lipopolysaccharidesmannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase (TIGR01479; EC 2.7.7.13,5.3.1.8; HMM-score: 13.5)
    molybdenum cofactor cytidylyltransferase (TIGR03310; EC 2.7.7.76; HMM-score: 13.4)
    Hypothetical proteinsConservedTIGR00454 family protein (TIGR00454; HMM-score: 12.8)
  • TheSEED:  
    Cell Wall and CapsuleCapsular and extracellular polysacchridesStreptococcal Hyaluronic Acid Capsule UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) 
  • PFAM:
    GT-A (CL0110) NTP_transferase; Nucleotidyl transferase (PF00483; HMM-score: 98.7)
    NTP_transf_3; MobA-like NTP transferase domain (PF12804; HMM-score: 26)
    Glycos_transf_2; Glycosyl transferase family 2 (PF00535; HMM-score: 14.1)
    no clan definedATP-gua_PtransN; ATP:guanido phosphotransferase, N-terminal domain (PF02807; HMM-score: 12.6)

Structure, modifications & interactions[edit source | edit]

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.101
    • Ymax_pos: 18
    • Cmax: 0.145
    • Cmax_pos: 36
    • Smax: 0.136
    • Smax_pos: 16
    • Smean: 0.084
    • D: 0.094
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLPILDKPTIQYIVEEAARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYVRQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQEVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLKTQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMREELTRFIKALGL

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. 1.000 1.001 1.002 1.003 1.004 1.005 1.006 1.007 1.008 1.009 1.010 1.011 1.012 1.013 1.014 1.015 1.016 1.017 1.018 1.019 1.020 1.021 1.022 1.023 1.024 1.025 1.026 1.027 1.028 1.029 1.030 1.031 1.032 1.033 1.034 1.035 1.036 1.037 1.038 1.039 1.040 1.041 1.042 1.043 1.044 1.045 1.046 1.047 1.048 1.049 1.050 1.051 1.052 1.053 1.054 1.055 1.056 1.057 1.058 1.059 1.060 1.061 1.062 1.063 1.064 1.065 1.066 1.067 1.068 1.069 1.070 1.071 1.072 1.073 1.074 1.075 1.076 1.077 1.078 1.079 1.080 1.081 1.082 1.083 1.084 1.085 1.086 1.087 1.088 1.089 1.090 1.091 1.092 1.093 1.094 1.095 1.096 1.097 1.098 1.099 1.100 1.101 1.102 1.103 1.104 1.105 1.106 1.107 1.108 1.109 1.110 1.111 1.112 1.113 1.114 1.115 1.116 1.117 1.118 1.119 1.120 1.121 1.122 1.123 1.124 1.125 1.126 1.127 1.128 1.129 1.130 1.131 1.132 1.133 1.134 1.135 1.136 1.137 1.138 1.139 1.140 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J. Proteome Res.: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)
  2. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  4. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Angelika Gründling, Olaf Schneewind
Genes required for glycolipid synthesis and lipoteichoic acid anchoring in Staphylococcus aureus.
J. Bacteriol.: 2007, 189(6);2521-30
[PubMed:17209021] [WorldCat.org] [DOI] (P p)