From AureoWiki
Jump to: navigation, search

NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02904
  • pan locus tag?: SAUPAN006262000
  • symbol: SAOUHSC_02904
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02904
  • symbol: SAOUHSC_02904
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 2672670..2673776
  • length: 1107
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    ATGCAACATCATAAAGTGGCTATTATCGGTGCCGGTGCTGCAGGTATAGGTATGGCCATT
    ACCTTAAAAGATTTCGGTATAACAGATGTCATTATTTTAGAAAAAGGAACAGTAGGACAT
    TCATTTAAACATTGGCCGAAATCGACCCGTACGATCACGCCATCATTTACGTCTAATGGA
    TTTGGCATGCCTGATATGAATGCAATTTCCATGGATACTTCACCAGCATTTACATTTAAT
    GAAGAACATATTTCCGGAGAAACATATGCTGAATATTTACAAGTGGTTGCCAACCATTAC
    GAGCTGAATATCTTTGAAAATACAGTTGTCACAAATATATCTGTAGATGATGCATATTAT
    ACGATTGCAACGACAACAGAGATATATCACGCGGATTATATCTTTGTCGCAACAGGTGAT
    TATAATTTCCCTAAAAAGCCATTTAAATATGGTATTCATTATAGTGAAATTGAAGACTTT
    GATAACTTTAATAAGGGGCAATATGTGGTTATCGGAGGTAATGAAAGTGGCTTTGATGCT
    GCATATCAACTTGCAAAAAATGGCTCTGACATCGCACTTTATACTAGCACAACCGGTTTA
    AATGATCCGGATGCTGATCCTAGTGTTAGATTGTCACCTTATACACGTCAGCGACTAGGT
    AATGTCATTAAGCAAGGTGCTCGCATCGAAATGAATGTACATTATACAGTTAAAGATATT
    GATTTTAACAATGGACAGTATCATATCAGTTTTGATAGCGGACAAAGTGTGCTTACACCT
    CATGAACCAATACTAGCAACTGGCTTTGATGCAACAAAAAATCCAATCGTTCAACAATTA
    TTTGTGACAACAAATCAAGATATTAAATTAACAACACATGATGAATCGACACGTTATCCG
    AATATTTTTATGATTGGTGCAACAGTTGAAAATGATAATGCCAAATTATGCTATATCTAT
    AAATTTAGAGCGCGATTTGCAGTACTTGCACATCTTTTAACACAGCGGGAAGGCTTACCA
    GCTAAACAAGATGTCATTGAAAATTATCAAAAAAATCAAATGTATTTAGATGATTATTCA
    TGTTGTGAAGTGTCATGCACATGTTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1107

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02904
  • symbol: SAOUHSC_02904
  • description: hypothetical protein
  • length: 368
  • theoretical pI: 5.0566
  • theoretical MW: 41168.8
  • GRAVY: -0.294565

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Unknown functionEnzymes of unknown specificityputative bacillithiol system oxidoreductase, YpdA family (TIGR04018; EC 1.8.-.-; HMM-score: 94.3)
    flavin-dependent oxidoreductase, MSMEG_0569 family (TIGR04046; HMM-score: 51.8)
    Unknown functionEnzymes of unknown specificityflavoprotein, HI0933 family (TIGR00275; HMM-score: 36.6)
    MetabolismEnergy metabolismElectron transportthioredoxin-disulfide reductase (TIGR01292; EC 1.8.1.9; HMM-score: 32.3)
    Cellular processesCellular processesDetoxificationmercury(II) reductase (TIGR02053; EC 1.16.1.1; HMM-score: 25.8)
    Cellular processesCellular processesDetoxificationalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 23.7)
    Cellular processesCellular processesAdaptations to atypical conditionsalkyl hydroperoxide reductase subunit F (TIGR03140; EC 1.8.1.-; HMM-score: 23.7)
    squalene-associated FAD-dependent desaturase (TIGR03467; HMM-score: 20.1)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase family (TIGR02032; EC 1.3.1.-; HMM-score: 19.7)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiaminethiazole biosynthesis enzyme (TIGR00292; HMM-score: 19.4)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPyridine nucleotidesL-aspartate oxidase (TIGR00551; EC 1.4.3.16; HMM-score: 19.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinone2-polyprenyl-6-methoxyphenol 4-hydroxylase (TIGR01984; EC 1.14.13.-; HMM-score: 18.9)
    dihydrolipoyl dehydrogenase (TIGR01350; EC 1.8.1.4; HMM-score: 18.6)
    putative selenate reductase, YgfK subunit (TIGR03315; HMM-score: 17.9)
    MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, beta subunit family (TIGR01373; HMM-score: 17.8)
    MetabolismEnergy metabolismAmino acids and aminessarcosine oxidase, monomeric form (TIGR01377; HMM-score: 17.7)
    MetabolismEnergy metabolismElectron transportflavocytochrome c (TIGR01813; HMM-score: 16.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersChlorophyll and bacteriochlorphyllgeranylgeranyl reductase (TIGR02023; EC 1.3.1.-; HMM-score: 15)
    MetabolismAmino acid biosynthesisPyruvate familyketol-acid reductoisomerase (TIGR00465; EC 1.1.1.86; HMM-score: 14.6)
    glutamate synthase, small subunit (TIGR01318; HMM-score: 14.2)
    putative alkyl hydroperoxide reductase F subunit (TIGR03143; EC 1.6.4.-; HMM-score: 14.1)
    pyridine nucleotide-disulfide oxidoreductase family protein (TIGR03169; HMM-score: 13.4)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain (TIGR03197; HMM-score: 13.4)
    MetabolismCentral intermediary metabolismNitrogen metabolismnitrite reductase [NAD(P)H], large subunit (TIGR02374; EC 1.7.1.4; HMM-score: 13.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersThiamineglycine oxidase ThiO (TIGR02352; EC 1.4.3.19; HMM-score: 12.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersOtherphytoene desaturase (TIGR02734; EC 1.14.99.-; HMM-score: 12.4)
    MetabolismAmino acid biosynthesisGlutamate familyglutamate synthase (NADPH), homotetrameric (TIGR01316; EC 1.4.1.13; HMM-score: 12.2)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersMenaquinone and ubiquinoneubiquinone biosynthesis hydroxylase, UbiH/UbiF/VisC/COQ6 family (TIGR01988; EC 1.14.13.-; HMM-score: 12.2)
    MetabolismEnergy metabolismElectron transportglutathione-disulfide reductase (TIGR01421; EC 1.8.1.7; HMM-score: 11.9)
    glutamate synthase, NADH/NADPH, small subunit (TIGR01317; EC 1.4.1.-; HMM-score: 11.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminprotoporphyrinogen oxidase (TIGR00562; EC 1.3.3.4; HMM-score: 11.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersPantothenate and coenzyme A2-dehydropantoate 2-reductase (TIGR00745; EC 1.1.1.-; HMM-score: 11.5)
    Genetic information processingProtein synthesistRNA and rRNA base modificationtRNA:m(5)U-54 methyltransferase (TIGR00137; EC 2.1.1.74; HMM-score: 11.1)
    9,9'-di-cis-zeta-carotene desaturase (TIGR02732; EC 1.3.5.6; HMM-score: 11.1)
    MetabolismEnergy metabolismAnaerobicglycerol-3-phosphate dehydrogenase, anaerobic, B subunit (TIGR03378; EC 1.1.5.3; HMM-score: 9.5)
  • TheSEED:  
    FIG016210: probable monooxygenase 
  • PFAM:
    NADP_Rossmann (CL0063) Pyr_redox_3; Pyridine nucleotide-disulphide oxidoreductase (PF13738; HMM-score: 324.8)
    Pyr_redox_2; Pyridine nucleotide-disulphide oxidoreductase (PF07992; HMM-score: 58.9)
    HI0933_like; HI0933-like protein (PF03486; HMM-score: 30.2)
    GIDA; Glucose inhibited division protein A (PF01134; HMM-score: 29.6)
    DAO; FAD dependent oxidoreductase (PF01266; HMM-score: 29.4)
    FAD_binding_2; FAD binding domain (PF00890; HMM-score: 27.5)
    K_oxygenase; L-lysine 6-monooxygenase (NADPH-requiring) (PF13434; HMM-score: 24.5)
    NAD_binding_8; NAD(P)-binding Rossmann-like domain (PF13450; HMM-score: 21.2)
    NAD_binding_9; FAD-NAD(P)-binding (PF13454; HMM-score: 20.4)
    Thi4; Thi4 family (PF01946; HMM-score: 19.7)
    Pyr_redox; Pyridine nucleotide-disulphide oxidoreductase (PF00070; HMM-score: 19.4)
    IlvN; Acetohydroxy acid isomeroreductase, NADPH-binding domain (PF07991; HMM-score: 17.4)
    NAD_binding_7; Putative NAD(P)-binding (PF13241; HMM-score: 14.5)
    FAD_binding_3; FAD binding domain (PF01494; HMM-score: 13.9)
    FAD_oxidored; FAD dependent oxidoreductase (PF12831; HMM-score: 11.4)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 0.83
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.155
    • Ymax_pos: 11
    • Cmax: 0.136
    • Cmax_pos: 41
    • Smax: 0.318
    • Smax_pos: 5
    • Smean: 0.233
    • D: 0.186
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKHWPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYELNIFENTVVTNISVDDAYYTIATTTEIYHADYIFVATGDYNFPKKPFKYGIHYSEIEDFDNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQRLGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPIVQQLFVTTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLTQREGLPAKQDVIENYQKNQMYLDDYSCCEVSCTC

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]