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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02924
  • pan locus tag?: SAUPAN006301000
  • symbol: SAOUHSC_02924
  • pan gene symbol?:
  • synonym:
  • product: 4-aminobutyrate aminotransferase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02924
  • symbol: SAOUHSC_02924
  • product: 4-aminobutyrate aminotransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2689521..2690858
  • length: 1338
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGTAAAGCACATCAATTAATTCAAGAGGATGAACATTATTTTGCGAAATCAGGACGT
    ATTAAATATTATCCGTTAGTGATTGATCATGGATATGGAGCAACATTGGTTGATATTGAG
    GGGAAGACATATATCGATTTGTTATCGAGTGCGAGTTCTCAAAACGTAGGTCATGCACCT
    AGAGAAGTAACAGAAGCGATAAAGGCACAAGTTGATAAATTTATTCATTATACACCAGCA
    TATATGTATCATGAACCGTTGGTACGTTTAGCTAAGAAGCTTTGTGAGATTGCACCTGGA
    GATTTTGAAAAAAGAGTGACCTTCGGATTAACCGGATCAGACGCAAATGATGGCATCATT
    AAATTTGCCAGAGCATATACAGGGCGTCCTTATATCATTAGTTTCACTAATGCATATCAT
    GGTTCAACTTTTGGCTCATTGTCTATGTCAGCTATTAGTTTAAATATGCGCAAACATTAT
    GGTCCGTTATTGAATGGTTTTTATCATATTCCGTTTCCAGATAAATATCGTGGTATGTAC
    GAGCAGCCACAAGCTAATTCAGTAGAAGAATATTTAGCACCCTTAAAAGAAATGTTTGCG
    AAGTATGTACCTGCTGACGAAGTAGCATGTATTGTTATTGAAACGATACAAGGCGATGGT
    GGACTTTTAGAACCAGTTCCAGGGTATTTTGAAGCGTTAGAAAAGATTTGTCGTGAACAT
    GGTATTTTAATCGCTGTCGATGATATTCAACAAGGTTTTGGGAGAACAGGTACATGGAGT
    TCAGTCTCGCATTTTAATTTTACGCCTGATTTAATCACTTTCGGAAAATCCTTAGCAGGT
    GGTATGCCTATGTCAGCAATTGTTGGACGCAAAGAGATTATGAATTGTTTAGAAGCACCA
    GCACATTTATTTACAACAGGTGCTAATCCAGTTAGTTGTGAAGCTGCATTAGCCACAATT
    CAAATGATTGAAGATCAGTCGCTTCTTCAGGCTAGTGCGGAAAAAGGGGAATATGTTAGG
    AAACGAATGGATCAATGGGTATCTAAATACAATAGTGTAGGCGATGTTAGAGGTAAAGGT
    CTGAGCATTGGTATTGATATTGTTTCCGACAAAAAACTCAAAACACGTGATGCCAGTGCG
    GCACTTAAAATTTGTAATTACTGCTTTGAGCATGGCGTAGTTATTATAGCTGTAGCAGGA
    AATGTGTTGCGATTCCAACCGCCATTGGTAATAACATATGAGCAATTAGACACGGCGTTA
    AACACTATAGAAGATGCACTGACTGCTTTGGAAGCAGGTAACTTAGATCAATATGACATA
    TCTGGACAAGGTTGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1338

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02924
  • symbol: SAOUHSC_02924
  • description: 4-aminobutyrate aminotransferase
  • length: 445
  • theoretical pI: 5.90753
  • theoretical MW: 49081.7
  • GRAVY: -0.119326

Function[edit source | edit]

  • reaction:
    EC 2.6.1.19?  ExPASy
    4-aminobutyrate--2-oxoglutarate transaminase4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate
  • TIGRFAM:
    MetabolismCentral intermediary metabolismOther4-aminobutyrate transaminase (TIGR00700; EC 2.6.1.19; HMM-score: 339.6)
    transaminase, acetylornithine/succinylornithine family (TIGR00707; HMM-score: 300.5)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotinadenosylmethionine-8-amino-7-oxononanoate transaminase (TIGR00508; EC 2.6.1.62; HMM-score: 255.1)
    ornithine--oxo-acid transaminase (TIGR01885; EC 2.6.1.13; HMM-score: 242.4)
    MetabolismCentral intermediary metabolismOther2,4-diaminobutyrate 4-transaminase (TIGR00709; EC 2.6.1.-; HMM-score: 226.8)
    Cellular processesCellular processesAdaptations to atypical conditionsdiaminobutyrate--2-oxoglutarate aminotransferase (TIGR02407; EC 2.6.1.76; HMM-score: 215)
    MetabolismEnergy metabolismAmino acids and aminessuccinylornithine transaminase family (TIGR03246; EC 2.6.1.81; HMM-score: 212.4)
    MetabolismCentral intermediary metabolismPolyamine biosynthesisputrescine aminotransferase (TIGR03372; EC 2.6.1.82; HMM-score: 185.9)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersHeme, porphyrin, and cobalaminglutamate-1-semialdehyde-2,1-aminomutase (TIGR00713; EC 5.4.3.8; HMM-score: 167.9)
    L-lysine 6-transaminase (TIGR03251; EC 2.6.1.36; HMM-score: 132)
    MetabolismCentral intermediary metabolismOther4-aminobutyrate aminotransferase (TIGR00699; EC 2.6.1.19; HMM-score: 100.8)
    MetabolismBiosynthesis of cofactors, prosthetic groups, and carriersBiotin8-amino-7-oxononanoate synthase (TIGR00858; EC 2.3.1.47; HMM-score: 30.2)
    putative pyridoxal phosphate-dependent acyltransferase (TIGR01825; EC 2.3.1.-; HMM-score: 20)
  • TheSEED:  
    aminotransferase 
  • PFAM:
    PLP_aminotran (CL0061) Aminotran_3; Aminotransferase class-III (PF00202; HMM-score: 351.6)
    Aminotran_1_2; Aminotransferase class I and II (PF00155; HMM-score: 13.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.67
    • Cytoplasmic Membrane Score: 0.01
    • Cellwall Score: 0.15
    • Extracellular Score: 0.17
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.108
    • Ymax_pos: 62
    • Cmax: 0.128
    • Cmax_pos: 62
    • Smax: 0.122
    • Smax_pos: 6
    • Smean: 0.081
    • D: 0.098
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTYIDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCEIAPGDFEKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHYGPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQGDGGLLEPVPGYFEALEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGKSLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAEKGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHGVVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

  • SAOUHSC_02924 no polycistronic organisation predicted

Regulation[edit source | edit]

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]