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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02955
  • pan locus tag?: SAUPAN006337000
  • symbol: nsaS
  • pan gene symbol?: nsaS
  • synonym:
  • product: nisin susceptibility-associated sensor histidine kinase

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02955
  • symbol: nsaS
  • product: nisin susceptibility-associated sensor histidine kinase
  • replicon: chromosome
  • strand: -
  • coordinates: 2717697..2718584
  • length: 888
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGACCTTTCTTAAAAGTATTACTCAGGAAATAGCAATAGTCATAGTTATTTTTGCTTTG
    TTTGGCTTAATGTTTTACCTGTATCATTTGCCATTAGAAGCATATTTACTAGCACTTGGC
    GTTATTTTATTATTATTACTCATATTCATAGGTATTAAATATTTAAGTTTTGTAAAAACT
    ATAAGCCAACAACAACAAATTGAAAACTTAGAAAATGCGTTGTATCAGCTTAAAAATGAA
    CAAATTGAATATAAAAATGATGTAGAGAGCTACTTTTTAACATGGGTACATCAAATGAAA
    ACACCCATTACTGCAGCACAACTGTTACTTGAAAGAGATGAGCCTAATGTTGTTAATCGT
    GTTCGTCAAGAGGTTATTCAAATTGATAACTATACAAGTTTAGCACTTAGTTATTTAAAG
    TTATTAAATGAAACTTCTGATATTTCTGTCACTAAAATTTCGATTAATAATATCATTCGC
    CCAATTATTATGAAATATTCAATACAGTTTATTGATCAAAAAACAAAAATCCATTATGAA
    CCTTGTCATCACGAAGTATTAACTGACGTTAGATGGACCTCTTTAATGATAGAACAATTA
    ATAAATAATGCACTTAAGTATGCGAGAGGTAAAGATATATGGATTGAATTTGATGAGCAA
    TCCAATCAATTACACGTAAAAGATAATGGTATCGGTATTAGTGAAGCGGACTTGCCTAAA
    ATATTTGATAAGGGCTATTCAGGTTATAATGGCCAGCGCCAAAGTAACTCAAGTGGGATT
    GGTTTATTTATCGTAAAACAAATTTCAACACACACAAACCATCCTGTTTCAGTCGTATCT
    AAACAAAATGAGGGTACAACATTTACGATTCAATTTCCAGATGAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    888

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02955
  • symbol: NsaS
  • description: nisin susceptibility-associated sensor histidine kinase
  • length: 295
  • theoretical pI: 6.09533
  • theoretical MW: 34019
  • GRAVY: -0.0125424

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Signal transductionSignal transductionTwo-component systemsphosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 62.9)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 49.3)
    Signal transductionSignal transductionTwo-component systemsTMAO reductase sytem sensor TorS (TIGR02956; EC 2.7.13.3; HMM-score: 43.7)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 31.3)
    Signal transductionSignal transductionTwo-component systemsputative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 31.3)
    MetabolismCentral intermediary metabolismNitrogen fixationnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 30.3)
    Signal transductionRegulatory functionsProtein interactionsnitrogen fixation negative regulator NifL (TIGR02938; HMM-score: 30.3)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA mismatch repair protein MutL (TIGR00585; HMM-score: 20.1)
    Genetic information processingDNA metabolismDNA replication, recombination, and repairDNA topoisomerase VI, B subunit (TIGR01052; EC 5.99.1.3; HMM-score: 17.5)
  • TheSEED:  
    Putative two-component sensor histidine kinase 
  • PFAM:
    His_Kinase_A (CL0025) HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 73.1)
    HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 26.9)
    HATPase_c_2; Histidine kinase-like ATPase domain (PF13581; HMM-score: 14.7)
    no clan definedDUF1043; Protein of unknown function (DUF1043) (PF06295; HMM-score: 14.6)
    Phage_holin_3_6; Putative Actinobacterial Holin-X, holin superfamily III (PF07332; HMM-score: 14.5)
    FtsL (CL0225) DivIC; Septum formation initiator (PF04977; HMM-score: 13.8)
    C_Lectin (CL0056) Ly49; Ly49-like protein, N-terminal region (PF08391; HMM-score: 13.6)
    His_Kinase_A (CL0025) HisKA; His Kinase A (phospho-acceptor) domain (PF00512; HMM-score: 12.5)
    no clan definedDUF4131; Domain of unknown function (DUF4131) (PF13567; HMM-score: 11.8)
    IncA; IncA protein (PF04156; HMM-score: 6.9)
    DUF3290; Protein of unknown function (DUF3290) (PF11694; HMM-score: 6.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.01
    • Cytoplasmic Membrane Score: 9.99
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 2
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.124
    • Ymax_pos: 65
    • Cmax: 0.125
    • Cmax_pos: 65
    • Smax: 0.189
    • Smax_pos: 60
    • Smean: 0.06
    • D: 0.099
  • predicted transmembrane helices (TMHMM): 2

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILLLLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTPITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIRPIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFDEQSNQLHVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPVSVVSKQNEGTTFTIQFPDE

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-3661
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Katy L Blake, Chris P Randall, Alex J O'Neill
In vitro studies indicate a high resistance potential for the lantibiotic nisin in Staphylococcus aureus and define a genetic basis for nisin resistance.
Antimicrob. Agents Chemother.: 2011, 55(5);2362-8
[PubMed:21300840] [WorldCat.org] [DOI] (I p)
Stacey L Kolar, Vijayaraj Nagarajan, Anna Oszmiana, Frances E Rivera, Halie K Miller, Jessica E Davenport, James T Riordan, Jan Potempa, David S Barber, Joanna Koziel, Mohamed O Elasri, Lindsey N Shaw
NsaRS is a cell-envelope-stress-sensing two-component system of Staphylococcus aureus.
Microbiology (Reading, Engl.): 2011, 157(Pt 8);2206-19
[PubMed:21565927] [WorldCat.org] [DOI] (I p)