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NCBI: 03-AUG-2016

Summary[edit source | edit]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02989
  • pan locus tag?: SAUPAN006389000
  • symbol: secY
  • pan gene symbol?: secY2
  • synonym:
  • product: accessory Sec system protein translocase subunit SecY2

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAOUHSC_02989
  • symbol: secY
  • product: accessory Sec system protein translocase subunit SecY2
  • replicon: chromosome
  • strand: -
  • coordinates: 2758588..2759799
  • length: 1212
  • essential: no DEG other strains

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGTTAAAACTTTTACAACAATACGAATATAAAATTATATATAAACGTATGCTATACACA
    TGTTTTATCTTGTTTATTTATATATTAGGCACAAACATTTCCATCGTTTCCTATAACGAT
    ATGCAAGTAAAACATGAGTCATTTTTTAAAATAGCTATTTCTAATATGGGTGGAGACGTT
    AATACTTTAAACATTTTCACTTTAGGATTAGGTCCGTGGTTAACATCAATGATTATCTTG
    ATGCTGATTTCTTATCGGAATATGGATAAATACATGAAACAAACAAGTCTTGAGAAGCAT
    TATAAAGAGCGCATCTTAACATTAATTTTAAGTGTTATTCAAAGTTATTTTGTGATTCAT
    GAATATGTTTCGAAAGAACGTGTTCATCAAGACAATATTTACTTAACAATATTGATTTTG
    GTTACTGGAACGATGTTATTAGTTTGGTTAGCTGACAAAAATAGTCGCTACGGCATCGCA
    GGACCAATGCCGATTGTAATGGTTAGTATTATTAAATCAATGATGCATCAAAAAATGGAA
    TATATAGATGCAAGTCATATTGTGATTGCGTTGTTAATCATCCTTGTGATAATCACATTG
    TTTATCTTATTATTTATTGAATTAGTAGAAGTGAGAATACCATATATCGATTTAATGAAC
    GTTTCCGCAACAAATATGAAATCTTATTTATCTTGGAAAGTTAACCCTGCAGGCAGTATT
    ACTTTAATGATGAGTATTTCAGCTTTTGTTTTCTTAAAAAGTGGCATTCATTTTATTTTA
    TCTATGTTTAATAAAAGCATATCAGATGACATGCCAATGCTGACATTTGATAGTCCAGTA
    GGTATTTCAGTATATTTAGTGATTCAAATGTTATTGGGTTATTTTTTATCGAGATTTTTA
    ATCAATACTAAACAAAAATCCAAAGATTTCTTAAAGAGTGGCAATTATTTTTCAGGAGTT
    AAACCTGGTAAGGATACAGAACGTTATTTAAATTATCAAGCACGACGCGTATGTTGGTTT
    GGATTGGCATTAGTTACAGTCATTATTGGTATACCGCTTTATTTCACATTGTTTGTACCG
    CATTTATCTACTGAAATTTATTTCTCAGTACAACTGATTGTATTAGTTTACATCAGTATT
    AATATTGCAGAAACAATTCGTACATATTTATATTTTGATAAATATAAGCCATTTTTAAAC
    CAGTATTGGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1212

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAOUHSC_02989
  • symbol: SecY
  • description: accessory Sec system protein translocase subunit SecY2
  • length: 403
  • theoretical pI: 9.60127
  • theoretical MW: 46884.8
  • GRAVY: 0.527543

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
    Genetic information processingProtein fateProtein and peptide secretion and traffickingaccessory Sec system translocase SecY2 (TIGR02920; HMM-score: 563.6)
    Genetic information processingProtein fateProtein and peptide secretion and traffickingpreprotein translocase, SecY subunit (TIGR00967; HMM-score: 121.5)
  • TheSEED:  
    Membrane TransportProtein translocation across cytoplasmic membraneSecY2-SecA2 Specialized Transport System Preprotein translocase SecY2 subunit (TC 3.A.5.1.1) 
  • PFAM:
    no clan definedSecY; SecY translocase (PF00344; HMM-score: 148.3)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 8
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.136
    • Ymax_pos: 39
    • Cmax: 0.146
    • Cmax_pos: 39
    • Smax: 0.206
    • Smax_pos: 7
    • Smean: 0.115
    • D: 0.128
  • predicted transmembrane helices (TMHMM): 10

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVKHESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYKERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIAGPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDLMNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKSISDDMPMLTFDSPVGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQARRVCWFGLALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYFDKYKPFLNQYW

Experimental data[edit source | edit]

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

  1. Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  2. Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet.: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

Relevant publications[edit source | edit]

Ian R Siboo, Donald O Chaffin, Craig E Rubens, Paul M Sullam
Characterization of the accessory Sec system of Staphylococcus aureus.
J. Bacteriol.: 2008, 190(18);6188-96
[PubMed:18621893] [WorldCat.org] [DOI] (I p)
Mark J J B Sibbald, Theresa Winter, Magdalena M van der Kooi-Pol, G Buist, E Tsompanidou, Tjibbe Bosma, Tina Schäfer, Knut Ohlsen, Michael Hecker, Haike Antelmann, Susanne Engelmann, Jan Maarten van Dijl
Synthetic effects of secG and secY2 mutations on exoproteome biogenesis in Staphylococcus aureus.
J. Bacteriol.: 2010, 192(14);3788-800
[PubMed:20472795] [WorldCat.org] [DOI] (I p)