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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40

NCBI: 27-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SAP011 [new locus tag: SA_RS00045 ]
  • pan locus tag?:
  • symbol: blaR1
  • pan gene symbol?:
  • synonym:
  • product: bla regulator protein blaR1

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAP011 [new locus tag: SA_RS00045 ]
  • symbol: blaR1
  • product: bla regulator protein blaR1
  • replicon: pN315
  • strand: +
  • coordinates: 8897..10654
  • length: 1758
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGGCTAAATTACTAATAATGAGCGTAGTAAGCTTTTGTTTTATATTTCTATTGTTAGTA
    TTTTTTAGGTACATATTAAAACGCTATTTTAATTATAGTTTAAATTATAAAGTTTGGTAT
    CTAACTATGCTTGCTGGATTAATTCCTTTTATCCCAATTAAATTCTCTTTTATAAAATTT
    AATAATGTGAATAATCAAGCGCCCACAGTTGAAAGTAAGTCACACGATTTGAACCATAAC
    ATAAATACCACCAAACCTATTCAAGAGTTCACAACAGATATCCATAAGTTTAATTGGGAT
    TCAATTGATAATATCTGCACAGTTATTTGGATAGTTTTAGTTATTATTTTAAGTTTTAAA
    TTTTTGAAAGCCTTATTATATCTTAAATATTTAAAGAAACAGTCACTTTATCTAAACGAA
    AATGAAAAAAATAAAATAGATACGATACTTTTCAACCATCAATATAAAAAAAATATTGTG
    ATTCGAAAAGCAGAGGCTATTCAATCTCCAATAACTTTTTGGTATGGGAAATATATTATT
    TTGATTCCTAGTTCATATTTTAAAAGTGTAATTGATAAAAGACTAAAATATATAATACTG
    CATGAATATGCTCATGCTAAAAATAGAGATACTTTACATTTAATTATCTTTAATATCTTC
    TCTATTATTATGAGTTACAATCCATTAGTACATATTGTAAAAAGAAAGATAATTCATGAC
    AATGAAGTAGAAGCCGATAGATTTGTCCTTAATAATATTAACAAAAATGAATTTAAAACT
    TATGCGGAATCTATTATGGACTCCGTATTAAAAACCCCATTTTCCAACAAAAATATATTA
    AGCCATTCATTTAATGGTAAAAAATCATTACTCAAAAGTAGATTAATTAATATAAAAGAA
    GCCGATCTGAAAAAGCAGTCAAAATTAATTCTAATTTTTATTTGTATTTTTACTTTTTTT
    ATCATGATAATTCAAAGCCAGTTTTTAATGGGGCAATCCTTAACTGATTATAATTATAAA
    AAGCCCTTACAAAGCGATTACCAAATTTTAGATGAAAGTAAAAATTTTGGTTCAAATAGT
    GGATCTTTCGTTATGTACAGCATGAAGAAAGACAAATACTATATTTATAATGAAAAAGAA
    AGTAGAAAAAGATATTCTCCTGATTCAACTTACAAAATTTATTTAGCACTGTTTGGACTT
    GACCGTCATATTATAAGTGATAAAAATTCACGTATGTCATGGAATCATAAACATTATCTT
    TTCGAGTCTTGGAATAAGGAACAAGATTTAAATACAGCAATGCAAAATTCAGTTAATTGG
    TACTTCGAACGTATTAGTAATCAAATACCAAAGAACTATACTGCGGCTCAACTCAAGCAA
    TTAAATTATGGTAATGAAAATTTGGGAAGTTATAAAAGCTATTGGATGGAAGATAGTTTG
    AAAATATCTAATCTTGAACAAGTAATAGTTTTTAAAAATATGATGGAACAAAATAACCAT
    TTTAGTAAAAAAGCAAAGAATCAATTATCTTCTTCATTATTGATTAAGAAAAATGAAAAG
    TATGAACTGTATGGGAAAACAGGTACAGGTATAGTAAACGGGAAGTATAATAATGGGTGG
    TTTGTAGGTTACGTAATTACAAATCATGATAAGTATTATTTTGCTACACATTTATCAGAT
    GGAAACCCGTCTGGGAAAAATGCTGAATTAATAAGTGAAAAAATATTAAAAGGAATGGGT
    GTTTTAAATGACCAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1758

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAP011 [new locus tag: SA_RS00045 ]
  • symbol: BlaR1
  • description: bla regulator protein blaR1
  • length: 585
  • theoretical pI: 10.0095
  • theoretical MW: 69051.6
  • GRAVY: -0.281709

Function[edit source | edit]

  • TIGRFAM:
    Cell structureCell envelopeBiosynthesis and degradation of murein sacculus and peptidoglycanpenicillin-binding protein 2 (TIGR03423; HMM-score: 26.3)
  • TheSEED:  
    Phages, Prophages, Transposable elements, PlasmidsTransposable elementsTn552 Beta-lactamase regulatory sensor-transducer BlaR1 
  • PFAM:
    Peptidase_MA (CL0126) Peptidase_M56; BlaR1 peptidase M56 (PF05569; HMM-score: 309.4)
    and 2 more
    Beta-lactamase (CL0013) Transpeptidase; Penicillin binding protein transpeptidase domain (PF00905; HMM-score: 135.5)
    Peptidase_MA (CL0126) Peptidase_M48; Peptidase family M48 (PF01435; HMM-score: 12.1)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 4
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular Possibility: 0.17
    • Signal Peptide Possibility: -0.5
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.228
    • Ymax_pos: 11
    • Cmax: 0.119
    • Cmax_pos: 29
    • Smax: 0.64
    • Smax_pos: 2
    • Smean: 0.5
    • D: 0.334
  • predicted transmembrane helices (TMHMM): 6

Accession numbers[edit source | edit]

Protein sequence[edit source | edit]

  • MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTMLAGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNINTTKPIQEFTTDIHKFNWDSIDNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIVIRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFNIFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTPFSNKNILSHSFNGKKSLLKSRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSLTDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYKIYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIPKNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQLSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGKNAELISEKILKGMGVLNDQ

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator: BlaI (repression) regulon
    BlaI(TF)important in Beta-lactam resistance;RegPrecise

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]