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04-0298108BA0217611819-9771193COLECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50N315NCTC8325NewmanRF122ST398T0131TCH60TW20USA300_FPR3757USA300_TCH1516VC40
NCBI: 27-JUN-2013

Summary[edit source | edit]

  • organism: Staphylococcus aureus N315
  • locus tag: SAP014 [new locus tag: SA_RS00060 ]
  • pan locus tag?:
  • symbol: tnp
  • pan gene symbol?:
  • synonym:
  • product: hypothetical protein

Genome View[edit source | edit]

Gene[edit source | edit]

General[edit source | edit]

  • type: CDS
  • locus tag: SAP014 [new locus tag: SA_RS00060 ]
  • symbol: tnp
  • product: hypothetical protein
  • replicon: pN315
  • strand: +
  • coordinates: 11853..13295
  • length: 1443
  • essential: unknown

Accession numbers[edit source | edit]

Phenotype[edit source | edit]

  • Share your knowledge and add information here. [edit]

DNA sequence[edit source | edit]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGAAAAACAAGGAGAAGTATCTAACTAATTTTTCTGAAGCCAAACGTAAAAAAGCTACT
    CAAAAGTACAATATTATAAAACCTTTTATCCTAGGCGAACAATCTTTATCAAGTATTTCT
    AAAAGTAAAGGGATTGCGTTAAGCACCCTTTATAGATGGAATAAGTCATATAAAGAACAA
    GGACTTAAAGGACTAATCTATGCGACAAAAGCTGATAAAGGAACACGTAAAATAGAACCA
    AAGATAATTGATGAAATTGAACGCCTTGCGCTTATGAATAAAAGAAATAGTATAGCCACA
    ATTCATAGGAAAACCACTAATTACTGTAAAGAAAATAATTTTGATATACCAAGTTATAAA
    CAAATTTATAGTGTTATTAAAGCGATGCCTAAGTCTGTTATTGATTTCTCTCATCAGGGT
    GAAAAATACTATCAAAATAAGTACGATTTAATACAAATTAGAGAATCCTCGAGACCCAAT
    GAAATATGGCAAGCAGACCATACTTTGCTAGATATCTTTATATTAGACCAAAAAGGTAAT
    ATCAATAGACCATGGCTAACTATTATTATGGATGACTATAGTCGTGCAATTGCAGGCTAT
    TTCTTAAGTTTTGATGCCCCCAATGCTCAAAACACAGCATTAACACTACACCAAGCAATA
    TGGAATAAAAATGATACCAATTGGCCAGTGTGTGGTATACCCGAAAAATTCTACACAGAT
    CATGGAAGTGACTTTACTTCACATCATATGGAACAAGTCGCTATTGATTTGAAAATTAAC
    TTGATGTTTTCGAAAGTTGGTGTTCCTCGTGGTCGTGGAAAAATAGAACGATTCTTTCAG
    ACAGTTAATCAAACTTTCTTAGAACAACTGCCTGGATATATAAACAATAATGATACTCCT
    AGTAATCTAATAAATTTTCACAATTTTGAAGAAAAATTACGTTACTTTTTAATTGAAGAT
    TATAATCAAAAAGAACATAGTACTATTCATACCACGCCTATCAGTCGTTGGAATAGTAAT
    CACTTTTTCCCTAATATGCCGAGTAGCTTAGAACAACTTGATTTATTATTGTTGGAAATA
    CCTAAATCTAGAAAAGTTCATTCAGATGGCATTCATTTTCAAGGTTTTCGATACAGTAAT
    ACTAATCTAGCAGCTTATGTAGGTGAATATGTACTGATTCGTTATAACCCAAATGATATG
    GCTGAAATACGTGTGTTTTATAGAGATGAATTTCTTTGTACTGCTATATGTCCAGAAATA
    GCTGATTACTCCATAGATATAAAAGACATACAGCACGCTCGTAGTCAGAGAAGAAAACAC
    TTAAAACAAAATATTGCTAGTCCAAGTACCACGGAATTAATTAAGGAAGAAAAAAATTGT
    GGTTATTCACCTCAAGAAACGACTAAAAACGTCAAAAAATTAAAGAGGTATCGTAATGAC
    TAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1443

Protein[edit source | edit]

General[edit source | edit]

  • locus tag: SAP014 [new locus tag: SA_RS00060 ]
  • symbol: Tnp
  • description: hypothetical protein
  • length: 480
  • theoretical pI: 9.64625
  • theoretical MW: 56158.5
  • GRAVY: -0.68625

Function[edit source | edit]

  • reaction:
  • TIGRFAM:
  • TheSEED:  
    Phages, Prophages, Transposable elements, PlasmidsTransposable elementsTn552 Tn552 transposase 
  • PFAM:
    RNase_H (CL0219) rve; Integrase core domain (PF00665; HMM-score: 93.6)
    no clan definedMu-transpos_C; Mu transposase, C-terminal (PF09299; HMM-score: 61.2)
    HTH (CL0123) HTH_23; Homeodomain-like domain (PF13384; HMM-score: 23.2)
    HTH_32; Homeodomain-like domain (PF13565; HMM-score: 20.2)
    HTH_28; Helix-turn-helix domain (PF13518; HMM-score: 19.7)
    HTH_Tnp_1; Transposase (PF01527; HMM-score: 16.4)
    RNase_H (CL0219) rve_3; Integrase core domain (PF13683; HMM-score: 15.5)
    HTH (CL0123) HTH_Tnp_Mu_2; Mu DNA binding, I gamma subdomain (PF09039; HMM-score: 15.3)
    HTH_29; Winged helix-turn helix (PF13551; HMM-score: 15)
    LZ_Tnp_IS481; leucine-zipper of insertion element IS481 (PF13011; HMM-score: 14.5)
    ADP-ribosyl (CL0084) Pertussis_S1; Pertussis toxin, subunit 1 (PF02917; HMM-score: 12.8)
    LysM (CL0187) LysM; LysM domain (PF01476; HMM-score: 12.7)

Structure, modifications & interactions[edit source | edit]

  • domains:
  • modifications:
  • cofactors:
  • effectors:
  • protein partners:

Localization[edit source | edit]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular Possibility: 1
    • Signal Peptide Possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • Ymax: 0.175
    • Ymax_pos: 11
    • Cmax: 0.129
    • Cmax_pos: 47
    • Smax: 0.383
    • Smax_pos: 4
    • Smean: 0.312
    • D: 0.228
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit source | edit]

  • GI: 16119214 NCBI
  • RefSeq: NP_395550 NCBI
  • UniProt:

Protein sequence[edit source | edit]

  • MKNKEKYLTNFSEAKRKKATQKYNIIKPFILGEQSLSSISKSKGIALSTLYRWNKSYKEQGLKGLIYATKADKGTRKIEPKIIDEIERLALMNKRNSIATIHRKTTNYCKENNFDIPSYKQIYSVIKAMPKSVIDFSHQGEKYYQNKYDLIQIRESSRPNEIWQADHTLLDIFILDQKGNINRPWLTIIMDDYSRAIAGYFLSFDAPNAQNTALTLHQAIWNKNDTNWPVCGIPEKFYTDHGSDFTSHHMEQVAIDLKINLMFSKVGVPRGRGKIERFFQTVNQTFLEQLPGYINNNDTPSNLINFHNFEEKLRYFLIEDYNQKEHSTIHTTPISRWNSNHFFPNMPSSLEQLDLLLLEIPKSRKVHSDGIHFQGFRYSNTNLAAYVGEYVLIRYNPNDMAEIRVFYRDEFLCTAICPEIADYSIDIKDIQHARSQRRKHLKQNIASPSTTELIKEEKNCGYSPQETTKNVKKLKRYRND

Experimental data[edit source | edit]

  • experimentally validated: no data available

Expression & Regulation[edit source | edit]

Operon[edit source | edit]

Regulation[edit source | edit]

  • sigma factor:
  • regulator:

Transcription pattern[edit source | edit]

Protein synthesis (provided by Aureolib)[edit source | edit]

Protein stability[edit source | edit]

  • half-life: no data available

Biological Material[edit source | edit]

Mutants[edit source | edit]

Expression vector[edit source | edit]

lacZ fusion[edit source | edit]

GFP fusion[edit source | edit]

two-hybrid system[edit source | edit]

FLAG-tag construct[edit source | edit]

Antibody[edit source | edit]

Other Information[edit source | edit]

You are kindly invited to share additional interesting facts.

Literature[edit source | edit]

References[edit source | edit]

Relevant publications[edit source | edit]