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COLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 27-JUN-2013

Summary[edit | edit source]

  • organism: Staphylococcus aureus N315
  • locus tag: SAP025 [new locus tag: SA_RS00115 ]
  • pan locus tag?:
  • symbol: SAP025
  • pan gene symbol?:
  • synonym:
  • product: truncated Pre protein

Genome View[edit | edit source]

Gene[edit | edit source]

General[edit | edit source]

  • type: CDS
  • locus tag: SAP025 [new locus tag: SA_RS00115 ]
  • symbol: SAP025
  • product: truncated Pre protein
  • replicon: pN315
  • strand: +
  • coordinates: 20652..21206
  • length: 555
  • essential: unknown

Accession numbers[edit | edit source]

Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    TTGATACAAAACTACCAAAAATCATTAGATACGCTTAAAAAGCTTCTGAGTGTCATGTAT
    GAAATTAAAACTAAAAATGTAGGTGGATGGTTTCATAAAGAGAAGCAAGAAACAGGCAAT
    ATTGTAATTACTAAAACTTATTTTGAAAAATACACTAAACAAATTAAAGCAGCACAGATG
    ATATTAGATGATTATGAATGGATTAAAAGTGGGAAGTCATTAAAAAAATCAGAGAAACAA
    AATGAAAGTTTAGTTAATGAATTGACTAGTGTCCATATGGAAAATGAAAAGCTGGTTGAA
    GAATTTAATGATTTAGCACAACGTTATAATTATTTGTTGTCAGAGAATGAAAAGAAAGAT
    AAAGAACTAAATTATACGCTTAAGTTATTCAATCAAGTTTTTAAAATCATTAAAAGTATG
    ATGAAAGAAGAGCGTTATCATACGCTAATAAATCATATTGATAATCATTTAGACAATTCT
    AAAATACGTGAAGTAATGACGATAGATAACAATGATGAACAGTTCTTTAAGAAGAAATAT
    CAAGCACAAGAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    555

Protein[edit | edit source]

General[edit | edit source]

  • locus tag: SAP025 [new locus tag: SA_RS00115 ]
  • symbol: SAP025
  • description: truncated Pre protein
  • length: 184
  • theoretical pI: 9.04015
  • theoretical MW: 22149.2
  • GRAVY: -0.920109

Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • truncated Pre protein
  • PFAM:
    no clan defined ZapB; Cell division protein ZapB (PF06005; HMM-score: 15.9)
    DUF4407; Domain of unknown function (DUF4407) (PF14362; HMM-score: 13.1)
    and 5 more
    Spectrin; Spectrin repeat (PF00435; HMM-score: 12.3)
    Spc24; Spc24 subunit of Ndc80 (PF08286; HMM-score: 10.6)
    CREPT; Cell-cycle alteration and expression-elevated protein in tumour (PF16566; HMM-score: 9.5)
    DMPK_coil; DMPK coiled coil domain like (PF08826; HMM-score: 8.3)
    IncA; IncA protein (PF04156; HMM-score: 7.6)

Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

Localization[edit | edit source]

  • PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005409
    • TAT(Tat/SPI): 0.000235
    • LIPO(Sec/SPII): 0.001509
  • predicted transmembrane helices (TMHMM): 0

Accession numbers[edit | edit source]

Protein sequence[edit | edit source]

  • MIQNYQKSLDTLKKLLSVMYEIKTKNVGGWFHKEKQETGNIVITKTYFEKYTKQIKAAQMILDDYEWIKSGKSLKKSEKQNESLVNELTSVHMENEKLVEEFNDLAQRYNYLLSENEKKDKELNYTLKLFNQVFKIIKSMMKEERYHTLINHIDNHLDNSKIREVMTIDNNDEQFFKKKYQAQE

Experimental data[edit | edit source]

  • experimentally validated:
  • protein localization:
  • quantitative data / protein copy number per cell:
  • interaction partners:

Expression & Regulation[edit | edit source]

Operon[edit | edit source]

Regulation[edit | edit source]

  • regulator:

Transcription pattern[edit | edit source]

Protein synthesis (provided by Aureolib)[edit | edit source]

Protein stability[edit | edit source]

  • half-life: no data available

Biological Material[edit | edit source]

Mutants[edit | edit source]

Expression vector[edit | edit source]

lacZ fusion[edit | edit source]

GFP fusion[edit | edit source]

two-hybrid system[edit | edit source]

FLAG-tag construct[edit | edit source]

Antibody[edit | edit source]

Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

Literature[edit | edit source]

References[edit | edit source]

Relevant publications[edit | edit source]